本文選題：人類胚胎腦 + SVZ��； 參考：《福建醫(yī)科大學(xué)》2006年碩士論文

【摘要】： 目的研究缺氧缺血(hypoxic ischemic, HI)對妊娠中期人類胚胎腦腦室管膜下區(qū)(subventricular zone, SVZ)神經(jīng)干細(xì)胞(neural stem cells,NSCs)、神經(jīng)元、神經(jīng)膠質(zhì)細(xì)胞(星形膠質(zhì)細(xì)胞、少突膠質(zhì)祖細(xì)胞和小膠質(zhì)細(xì)胞)的影響,比較不同細(xì)胞對HI損傷的耐受性差異,揭示缺氧缺血腦損傷(Hypoxic-ischemic brain damaged , HIBD)及其它發(fā)育腦損傷性疾病的多種腦功能障礙表現(xiàn)的細(xì)胞學(xué)基礎(chǔ)。探討腦活素、NGF對HI損傷的SVZ神經(jīng)細(xì)胞的保護(hù)作用,為臨床用藥提供實(shí)驗(yàn)及理論依據(jù)。 方法取妊娠17~22孕周(gestational weeks,gw)的健康胚胎腦5例,即刻解離出SVZ細(xì)胞,以氧糖缺失法(oxygen/glucose deprivation, OGD)[1]建立HI損傷模型,計(jì)算HI組和正常組細(xì)胞成活率。將HI組和正常組SVZ細(xì)胞按A藥(NGF)、B藥(腦活素)兩因素,用藥、不用藥兩個(gè)水平,交叉分成4組培養(yǎng),培養(yǎng)4小時(shí)后以細(xì)胞特異性標(biāo)志蛋白nestin、MAP2、GFAP、PDGFRα及RCA120的抗體通過免疫熒光細(xì)胞化學(xué)法鑒定SVZ的5種主要細(xì)胞:NSCs、神經(jīng)元、星形膠質(zhì)細(xì)胞、少突膠質(zhì)祖細(xì)胞及小膠質(zhì)細(xì)胞。比較HI組和正常組5種細(xì)胞含量的改變,分析兩藥對HI和正常組SVZ細(xì)胞總數(shù)、對HI組SVZ神經(jīng)元數(shù)的影響。 結(jié)果妊娠中期人類胚胎腦SVZ中含有15.08±0.02%的nestin(+)NSCs、48.81±0.03%的MAP2(+)神經(jīng)元、32.31±0.03%的GFAP(+)星形膠質(zhì)細(xì)胞、6.92±0.02%的PDGFRα(+)少突膠質(zhì)祖細(xì)胞和1.15±0.01%的RCA120(+)小膠質(zhì)細(xì)胞。 細(xì)胞成活率:HI組為63.41±0.06%,正常組為98.9±0.01%。HI組細(xì)胞成活率明顯低于正常組,差異具有顯著性(p0.001),提示HI后SVZ細(xì)胞損傷明顯。 5種SVZ細(xì)胞的百分含量結(jié)果比較:nestin(+)的NSCs分別占HI組和正常
[Abstract]:Objective to study the effects of hypoxic ischemia (HIH) on neural stem cells, neurons and glial cells (astrocytes, oligodendrocytes and microglia) in the subventricular zone of human embryonic brain in the second trimester of pregnancy. By comparing the tolerance of different cells to HI injury, the cytological basis of Hypoxic-ischemic brain damaged (HIBDs) and other developmental brain damage disorders was revealed. To investigate the protective effect of brain activin on SVZ neurons injured by HI, and to provide experimental and theoretical basis for clinical use of NGF. Methods SVZ cells were dissociated from 5 healthy fetal brains of 17 weeks gestational weeks gestational week gestational week gestational gestational week of pregnancy. Oxygen-glucose deprivation (OGD1) was used to establish HI injury model, and the survival rate of HI group and normal group were calculated. The SVZ cells of HI group and normal group were divided into 4 groups according to the two factors of A drug NGF FG B (cerebrolysin), two levels of no medication and four groups of culture. After 4 hours of culture, the five major cells of SVZ were identified by immunocytochemistry with the antibodies to the cell specific marker protein nestin-MAP2GFAPR 偽 and RCA120. The five main cells of SVZ were identified as: NSCs, neurons, astrocytes, oligodendrocyte progenitors and microglial cells. The changes of 5 kinds of cells in HI group and normal group were compared, and the effects of the two drugs on the total number of SVZ cells and the number of SVZ neurons in HI group were analyzed. Results there were 15.08 鹵0.02% nestin-like neurons and 32.31 鹵0.03% astrocytes and 6.92 鹵0.02% PDGFR 偽 (oligodendrocyte) and 1.15 鹵0.01% RCA120 (microglia) in human embryonic brain SVZ in the second trimester of pregnancy. The survival rate of SVZ cells in HI group (63.41 鹵0.06) and normal group (98.9 鹵0.01%.HI) was significantly lower than that in normal group (P 0.001). Comparison of the percentage content of five kinds of SVZ cells in HI group and normal group
【學(xué)位授予單位】：福建醫(yī)科大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2006
【分類號】：R714.7;R363

【引證文獻(xiàn)】

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1 葉欣;陳日堅(jiān);莊大文;;施普善在急性腦梗死治療中的應(yīng)用[J];中國當(dāng)代醫(yī)藥;2011年25期

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