本文選題：鑭 + 氟　； 參考：《安徽醫(yī)科大學(xué)》2007年碩士論文

【摘要】： 1稀土元素鑭對大鼠免疫功能的影響 目的探討硝酸鑭對大鼠的淋巴細(xì)胞轉(zhuǎn)化率(LTR)、紅細(xì)胞免疫(紅細(xì)胞C_(3b)受體花環(huán)率,RC_(3b)RR)和巨噬細(xì)胞活性(PPM)等免疫功能的影響。方法64只40日齡的SD雄性大鼠,隨機(jī)分為實(shí)驗組Ⅰ,實(shí)驗組Ⅱ,實(shí)驗組Ⅲ和對照組,每組16只。實(shí)驗組灌飼硝酸鑭溶液,劑量依次為0.1,2,20mg/kg體重,每只2ml,1次/天。對照組灌飼生理鹽水,2ml/只,1次/天。10天后各組處死一半(共32只)做短期研究,30天后處死剩下的一半做長期研究。比較在十天(短期影響)和三十天(長期影響)后,各組大鼠的淋巴細(xì)胞轉(zhuǎn)化率、紅細(xì)胞C_(3b)受體花環(huán)率和巨噬細(xì)胞活性,并進(jìn)行差異的顯著性檢驗。結(jié)果短期影響:中等劑量的硝酸鑭(2mg/kg)組(實(shí)驗Ⅱ組)腹腔巨噬細(xì)胞吞噬活性有顯著提高(P＜0.05),而淋巴細(xì)胞轉(zhuǎn)化率,紅細(xì)胞C_(3b)受體花環(huán)率有提高趨勢,但未達(dá)到顯著水平(P＞0.05)。長期影響:低劑量(0.1mg/kg)的硝酸鑭組(實(shí)驗Ⅰ組)紅細(xì)胞C_(3b)受體花環(huán)率高于對照組(P＜0.05),而淋巴細(xì)胞轉(zhuǎn)化率和腹腔巨噬細(xì)胞吞噬活性均呈上升趨勢,但未達(dá)到顯著水平(P＞0.05);中等劑量(2mg/kg)的硝酸鑭組(實(shí)驗Ⅱ組)淋巴細(xì)胞轉(zhuǎn)化率、紅細(xì)胞C_(3b)受體花環(huán)率及其腹腔巨噬細(xì)胞吞噬活性均顯著高于對照組(P＜0.05);高等劑量(20mg/kg body weight)的硝酸鑭組(實(shí)驗Ⅲ組)淋巴細(xì)胞轉(zhuǎn)化率和腹腔巨噬細(xì)胞吞噬活性均低于對照組,但未達(dá)到顯著水平(P＞0.05)。結(jié)論大鼠飼喂硝酸鑭溶液在一定的時間和一定的劑量范圍內(nèi)能夠提高其免疫能力;中、低劑量的硝酸鑭對大鼠的免疫力有促進(jìn)作用,高劑量時則有抑制趨勢。 2稀土元素鑭對大鼠內(nèi)分泌功能的影響 目的探討硝酸鑭對大鼠內(nèi)分泌功能的影響。方法64只40日齡的SD雄性大鼠,隨機(jī)分為實(shí)驗組Ⅰ,實(shí)驗組Ⅱ,實(shí)驗組Ⅲ和對照組,每組16只。實(shí)驗組灌飼硝酸鑭溶液,劑量依次為0.1,2,20mg/kg體重,每只2ml,1次/天。對照組灌飼生理鹽水,2ml/只,1次/天。10天后各組處死一半(共32只)做短期研究,30天后處死剩下的一半做長期研究。比較在十天(短期影響)和三十天(長期影響)后,各組大鼠血清中甲狀腺激素(T_3、T_4)和胰島素(Ins)水平,并進(jìn)行差異的顯著性檢驗。結(jié)果短期影響:大鼠灌喂硝酸鑭,胰島素水平變化明顯,低劑量組(0.1mg/kg)、中劑量組(2mg/kg)和高劑量組(20mg/kg)的胰島素水平比對照組均有顯著升高(P＜0.05);甲狀腺激素(T_3、T_4)變化表現(xiàn)出低劑量組(0.1mg/kg)T_3分泌水平提高(P＜0.05)。長期影響:大鼠灌喂硝酸鑭,胰島素水平變化也非常明顯,低劑量組(0.1mg/kg)、中劑量組(2mg/kg)和高劑量組(20mg/kg)的胰島素水平比對照組均有顯著升高(P＜0.05):甲狀腺激素(T_3、T_4)無明顯變化(P＞0.05)。結(jié)論硝酸鑭對大鼠甲狀腺激素(T_3、T_4)的分泌無明顯影響,而顯著提高大鼠胰島素的分泌。 3微量元素氟對小鼠內(nèi)分泌功能的影響 目的探討微量元素氟及高氟補(bǔ)硒后對小鼠甲狀腺、胰島等內(nèi)分泌功能的影響。方法將80只昆明種小鼠(19-22g)隨機(jī)分為隨機(jī)分為8組,短期和長期各四組,這四組分別為對照組、實(shí)驗Ⅰ組(低氟組)、實(shí)驗Ⅱ組(高氟組)、實(shí)驗Ⅲ組(高氟+硒組)。10只/組。三個實(shí)驗組小鼠分別腹腔注射20mg/L氟化納溶液、200mg/L氟化納溶液以及200mg/L氟化納與100mg/L亞硒酸納的混合溶液,對照組小鼠腹腔注射生理鹽水。每只小鼠每天注射一次,每次1ml,短期實(shí)驗10天,長期實(shí)驗30天。比較在十天(短期影響)和三十天(長期影響)后,比較組小鼠血清中T_3、T_4及Ins水平的變化,并進(jìn)行差異的顯著性檢驗。結(jié)果短期影響:低氟組T_3、T_4及Ins水平與對照組相比,無顯著差異(P＞0.05);高氟組T_4和Ins水平高于對照組,有顯著差異(P＜0.05),T_3水平有升高趨勢,但無顯著差異(P＞0.05);高氟加硒組T_3、T_4及Ins水平與高氟組比較均有下降趨勢。長期影響:低氟組T_3、T_4及Ins水平與對照組相比,無顯著差異(P＞0.05);高氟組T_4和Ins水平低于對照組,有顯著差異(P＜0.05),T_3水平有下降趨勢,但無顯著差異(P＞0.05);高氟加硒組T_3、T_4水平與高氟組比較均有上升趨勢,Ins水平與高氟組比較有下降趨勢。結(jié)論高氟狀態(tài)可影響機(jī)體甲狀腺、胰島等內(nèi)分泌腺體的分泌功能。短期使甲狀腺興奮,分泌功能增強(qiáng),長期則抑制甲狀腺功能使其分泌功能減弱;高氟對胰島的分泌功能有刺激作用,可使其功能增強(qiáng)、胰島素分泌增加;甲狀腺、胰島等內(nèi)分泌腺功能的改變與氟的攝入量及攝入時間的長短存在一定關(guān)系;在高氟狀態(tài)下補(bǔ)硒后,使甲狀腺、胰島等內(nèi)分泌腺功能改變呈回復(fù)趨勢。
[Abstract]:The effect of Lanthanum on immune function of rats with 1 lanthanum
Objective to investigate the effects of lanthanum nitrate on lymphocyte transformation rate (LTR), red cell C_ (3b) receptor rosette rate, RC_ (3b) RR) and macrophage activity (PPM). Methods 64 40 day old SD male rats were randomly divided into experimental group I, experimental group II, experimental group III and control group, 16 rats in each group. The experimental group was fed with nitrate. The dose of lanthanum acid was 0.1,2,20mg/kg weight, each 2ml, 1 times per day. The control group was fed with physiological saline, 2ml/ only, 1 times / day.10 days were killed in half (a total of 32) for short-term study. 30 days later, the remaining half of the study was done. After ten days (short-term effect) and thirty days (long-term effect), the lymphocyte turns in each group. Rate, red cell C_ (3b) receptor rosette rate and macrophage activity, and the difference between the significant test. Results short term effect: the phagocytic activity of peritoneal macrophages in the medium dose (2mg/kg) group (experimental group II) increased significantly (P < 0.05), and the lymphatic cell transformation rate, the red cell C_ (3b) receptor rosette rate increased, but not reached. To a significant level (P > 0.05). Long term effect: the rate of red blood cell C_ (3b) receptor rosette in the low dose (0.1mg/kg) group of lanthanum nitrate (experimental group I) was higher than that of the control group (P < 0.05), but the lymphocyte transformation rate and the phagocytic activity of peritoneal macrophages were all rising, but not reached the significant level (P > 0.05), and the medium dose (2mg/kg) lanthanum nitrate group (Experiment II) Lymphocyte transformation rate, erythrocyte C_ (3b) receptor rosette rate and phagocytic activity of peritoneal macrophage were significantly higher than that of control group (P < 0.05). The lymphocyte transformation rate and phagocytic activity of peritoneal macrophages in the high dose (20mg/kg body weight) group of lanthanum nitrate group (experimental group III) were lower than those of the control group, but did not reach the significant level (P > 0.05). Conclusion the rats fed with lanthanum nitrate can improve their immune ability in a certain time and a certain dose range, and the low dose of lanthanum nitrate can promote the immunity of rats, and there is a trend of inhibition in high dose.
The effect of Lanthanum on the endocrine function of rats with 2 lanthanum
Objective to investigate the effect of lanthanum nitrate on the endocrine function of rats. Methods 64 SD male rats aged 40 days old were randomly divided into experimental group I, experimental group II, experimental group III and control group, 16 rats in each group. The experimental group was fed with lanthanum nitrate solution in order of 0.1,2,20mg/kg weight, each 2ml, 1 times. The control group was fed with physiological saline, 2ml/ only, 1 times / day.10 Half of each group died (32) for short term study. 30 days later, half of the rest were executed for long-term study. After ten days (short-term impact) and thirty days (long term effect), the serum levels of thyroid hormone (T_3, T_4) and insulin (Ins) were compared and the difference was observed. Results the short-term effect: rats fed with lanthanum nitrate, The insulin level in the low dose group (0.1mg/kg), the middle dose group (2mg/kg) and the high dose group (20mg/kg) were significantly higher than the control group (P < 0.05). The changes of thyroid hormone (T_3, T_4) showed the level of T_3 secreting in the low dose group (P < 0.05) (P < 0.05). The long-term effect: rats fed with lanthanum nitrate and insulin level The changes in the low dose group (0.1mg/kg), the middle dose group (2mg/kg) and the high dose group (20mg/kg) were significantly higher than those in the control group (P < 0.05): thyroid hormone (T_3, T_4) had no significant changes (P > 0.05). Conclusion lanthanum nitrate had no significant effect on the secretion of thyroid hormone (T_3, T_4) in rats, and significantly increased the islet of rats. The secretion of a hormone.
The effect of 3 trace element fluorine on the endocrine function of mice
Objective to investigate the effect of trace element fluorine and high fluorine supplementation on the endocrine function of the thyroid and islets of mice. Methods 80 Kunming mice (19-22g) were randomly divided into 8 groups, the short-term and the long-term four groups, the four groups were the control group, experimental group I (low fluorine group), experimental group II (Gao Fuzu), experimental group III (high fluoride + Se group).10 only / The mice in the three experimental groups were injected intraperitoneally with 20mg/L fluorine sodium solution, 200mg/L fluorine sodium solution and 200mg/L fluorine sodium and 100mg/L selenite. The control mice were injected intraperitoneally with saline. Each mouse was injected once a day, each time was 1ml, the short-term experiment was 10 days, and the long-term experiment was 30 days. The comparison was compared to ten days (short-term effect) and three. After ten days (long term influence), the changes of T_3, T_4 and Ins levels in the serum of mice were compared, and the significant difference between the levels of T_3, T_4 and Ins in the low fluorine group was not significant (P > 0.05), and the level of T_4 and Ins in the high fluorine group was higher than that in the control group (P < 0.05), but the T_3 level had a rising trend, but the level of T_3 was higher than that of the control group, but the level of T_3 was higher than that in the control group. There was no significant difference (P > 0.05). The level of T_3, T_4 and Ins in the high fluoride and selenium group had a downward trend compared with the high fluorine group. The long-term effect: the level of T_3, T_4 and Ins in the low fluorine group was no significant difference compared with the control group (P > 0.05), and the level of T_4 and Ins in the high fluorine group was lower than that of the control group (P < 0.05), and the T_3 level declined, but there was no significant difference (0. > > > > > > > > > > > > 0.. 05): the level of T_3 and T_4 in the high fluoride and selenium group had a rising trend compared with the high fluorine group. The level of Ins and the high fluorine group had a downward trend. Conclusion the high fluorine state could affect the secretory function of the thyroid gland and the pancreatic islets. The thyroid gland was excited and the secretory function was enhanced in the short term, and the secretion function of the thyroid gland was weakened for a long time. Fluorine has a stimulating effect on the secretory function of islets, which can enhance its function, increase the secretion of insulin, change the function of endocrine glands such as thyroid and islet, and have a certain relationship with the intake of fluorine and the length of the intake time.

【學(xué)位授予單位】：安徽醫(yī)科大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2007
【分類號】：R363

【參考文獻(xiàn)】

相關(guān)期刊論文 前10條

1 崔麗,黃可欣,聶毓秀,李艷茹;三氯化釤、三氯化鐠對小鼠腹腔巨噬細(xì)胞的影響[J];白求恩醫(yī)科大學(xué)學(xué)報;1999年05期

2 李樹蕾,聶毓秀,陳曦,黃可欣;硝酸鑭對大鼠胃粘膜長期作用的實(shí)驗研究[J];白求恩醫(yī)科大學(xué)學(xué)報;2001年02期

3 劉佳梅,陳東,王曉明,聶毓秀;硝酸鑭對大鼠脾臟形態(tài)及脾細(xì)胞IL-2和IFN-γ分泌水平的影響[J];吉林大學(xué)學(xué)報(醫(yī)學(xué)版);2002年02期

4 周莉，黃可欣，王曉輝，聶毓秀，王麗華，，唐毓環(huán);低劑量氯化鑭、氯化鐿對大鼠血清激素的影響[J];白求恩醫(yī)科大學(xué)學(xué)報;1996年02期

5 周莉，聶毓秀，黃可欣，朱秀雄;小劑量三氯化鐠對大鼠甲狀腺超微結(jié)構(gòu)和功能的影響[J];白求恩醫(yī)科大學(xué)學(xué)報;1996年02期

6 肖開棋,于燕妮,李若庭,王慶連,方德謙;慢性氟中毒患者及大鼠紅細(xì)胞掃描電鏡研究[J];地方病通報;1988年02期

7 陳培忠,云中杰,孫國棟,王玉濤,高紅旭,馬愛華,婁占玉;高氟對機(jī)體內(nèi)分泌激素水平的影響[J];地方病通報;2004年02期

8 王傳虎;元素氟與人體健康[J];地方病通報;2004年03期

9 譚郁彬;氟對機(jī)體代謝及各系統(tǒng)的影響[J];中國地方病防治雜志;1994年03期

10 邢德利;徐淑艷;張文嵐;李廣生;;過量攝氟對大鼠腦組織氧化侵襲與細(xì)胞凋亡的影響[J];中國地方病防治雜志;2006年01期

本文編號：1798308