本文選題：脫細胞細胞外基質(zhì)　切入點：雪旺細胞　出處：《中國醫(yī)科大學》2005年博士論文

【摘要】：背景 雖然橋接法修復長段神經(jīng)缺損的標準做法是神經(jīng)移植,但自體神經(jīng)移植可供橋接長段神經(jīng)缺損的供區(qū)有限,是一種創(chuàng)傷修復創(chuàng)傷,限制了臨床應(yīng)用。為了克服這一缺點,一直在繼續(xù)研究尋找更易接受的替代物。目前的研究認為神經(jīng)缺損的替代材料有:非生物材料PLA、PGA、PPE;生物材料:血管、膜管、變性肌肉、AECM;自體神經(jīng)移植:游離移植、帶血管蒂移植;組織工程:人工神經(jīng)。AECM抗原性低,且具備接近人體的網(wǎng)架結(jié)構(gòu)、良好的生物相容性、生物力學性能;FN,LN能較完整保留在細胞外基質(zhì)內(nèi),其表面具備細胞膜受體識別位點,有助于促進與雪旺細胞黏附和神經(jīng)軸突生長,因而是組織工程化人工神經(jīng)的理想支架。但應(yīng)用AECM修復缺損的距離受限制,組織工程學研究為解決周圍神經(jīng)修復難題帶來新的希望。 目的 本研究采用組織工程化技術(shù)制備的脫細胞細胞外基質(zhì)并行端側(cè)吻合法預(yù)構(gòu)建橋接物修復長段神經(jīng)缺損,并且雪旺細胞復合脫細胞細胞外基質(zhì)并行端側(cè)吻合法預(yù)構(gòu)建橋接物修復長段神經(jīng)缺損,對橋接長段神經(jīng)缺損的效果做了初步探索,旨在開發(fā)自體神經(jīng)移植的理想替代材料。為組織工程人工神經(jīng)的臨床應(yīng)用提供理論依據(jù)。 方法 第一部分:組織工程化神經(jīng)橋接物制備(化學萃取法);脫細胞細胞外
[Abstract]:BackgroundAlthough the standard method for repairing long segment nerve defect by bridging method is nerve transplantation, the donor area of autogenous nerve graft for bridging long segment nerve defect is limited, which is a kind of trauma repair trauma, which limits the clinical application.To overcome this shortcoming, research has continued to find more acceptable alternatives.Current studies suggest that alternative materials for neural defects are: non-biomaterial PLA PGAPPE; biomaterials: blood vessels, membrane tubes, denatured muscles AECM; autologous nerve transplantation: free transplantation with vascular pedicle; tissue engineering: artificial nerve. AECM has low antigenicity.In addition, it has close to human network structure, good biocompatibility, and its biomechanical properties can be completely preserved in extracellular matrix, and its surface has cell membrane receptor recognition site.It is helpful to promote the adhesion of Schwann cells and the growth of nerve axons, so it is an ideal scaffold for tissue engineering artificial nerve.However, the distance of repairing defects with AECM is limited, and the study of tissue engineering brings new hope to solve the problem of peripheral nerve repair.PurposeIn this study, an acellular extracellular matrix (ECM) with end-to-side anastomosis was prepared by tissue engineering technique to repair long nerve defects.In addition, Schwann cells combined with acellular extracellular matrix and end-to-side anastomosis were constructed to repair long segment nerve defects. The effect of bridging long segment nerve defects was preliminarily explored in order to develop an ideal alternative material for autogenous nerve transplantation.To provide a theoretical basis for the clinical application of tissue engineering artificial nerve.MethodPart one: preparation of tissue engineered nerve bridging material (chemical extraction; extracellular; extracellular)
【學位授予單位】：中國醫(yī)科大學
【學位級別】：博士
【學位授予年份】：2005
【分類號】：R329

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