本文選題：眼直肌　切入點：Pulley　出處：《天津醫(yī)科大學(xué)》2006年博士論文

【摘要】：目的 采用組織化學(xué)及免疫組織化學(xué)技術(shù)、全眼眶連續(xù)冠狀切片技術(shù)、計算機輔助三維重建技術(shù)研究人眼直肌Pulley的組織構(gòu)成及其在眼眶內(nèi)的立體空間分布情況,從多方面、多角度展示Pulley的組織形態(tài)結(jié)構(gòu),為探討Pulley功能提供解剖學(xué)和組織學(xué)依據(jù)。 方法 (1) 選取5例無眼部疾患的新鮮尸體的眼眶,整體固定包埋,進行全眼眶連續(xù)冠狀切片。相鄰層面經(jīng)Masson和Weigert兩種特殊染色及免疫組織化學(xué)染色后,從大體角度和在光鏡下觀察每個層面的組織結(jié)構(gòu)。 (2) 選取Pulley及Pulley連接帶典型的組織切片層面經(jīng)苦味酸一天狼星紅染色后,在偏振光顯微鏡下觀察直肌Pulley及Pulley連接帶的膠原組成成分及分布特點。并用Mias-2000圖像分析系統(tǒng)分析四條直肌Pulley及Pulley連接帶的膠原組成。 (3) 經(jīng)Masson染色的全眼眶冠狀位連續(xù)組織切片圖像用數(shù)碼相機攝像后逐一輸入計算機內(nèi),經(jīng)photoshop 7.0軟件除噪處理,應(yīng)用3D-Doctor軟件通過對圖像的定位、分割進行Pulley系統(tǒng)與眼球,四條直肌及眼眶壁的三維立體模型的重建。同時應(yīng)用3D-DOCTOR軟件的測量工具,對Pulley及相關(guān)組織結(jié)構(gòu)進行測量和分析。 結(jié)果 (1) Pulley位于眼球赤道部直肌穿過Tenon囊的部位,為纖維環(huán)狀結(jié)構(gòu),包繞每條直肌。各直肌Pulley之間形成連接帶。Pulley及其連接帶的主要成分為膠原纖維、彈力纖維及平滑肌。內(nèi)、外直肌Pulley通過與其成分相似的懸吊韌帶與眼眶壁相連。各直肌肌肉纖維由眶層和球?qū)咏M成,球?qū)蛹∪饫w維穿過
[Abstract]:PurposeHistochemical and immunohistochemical techniques, continuous coronal section of the whole orbit and computer aided three-dimensional reconstruction were used to study the tissue composition and three-dimensional spatial distribution of human rectus muscle (Pulley) in the orbit.In order to provide anatomical and histological basis for exploring the function of Pulley, the morphologic structure of Pulley was demonstrated from different angles.Method1) 5 cases of fresh cadavers without ocular diseases were fixed and embedded with coronal sections of the whole orbit.After Masson and Weigert special staining and immunohistochemical staining, the tissue structure of each layer was observed from the general angle and under light microscope.(2) the typical tissue sections of Pulley and Pulley junctional bands were stained with picric acid-Sirius red to observe the composition and distribution of collagen in the Pulley and Pulley junctions of rectus muscle under polarized light microscope.The collagen composition of Pulley and Pulley junctions of four rectus muscles were analyzed by Mias-2000 image analysis system.3) the whole orbital coronal continuous tissue slice image stained by Masson was recorded by digital camera and then input into the computer one by one. The image was segmented with Pulley system and eyeball by photoshop 7.0 software to remove noise, and 3D-Doctor software was used to segment the image.Reconstruction of three-dimensional models of four rectus muscles and orbital walls.At the same time, the measurement tool of 3D-DOCTOR software is used to measure and analyze the Pulley and related organization structure.ResultPulley is located in the equatorial rectus of the eye through the Tenon sac and is a fibrous annular structure that encircles each rectus muscle.The main components of the connective band. Pulley and its connective band are collagen fiber, elastic fiber and smooth muscle.The medial and outer rectus Pulley are connected to the orbital wall through a suspension ligament similar to its composition.The rectus muscle fibers are composed of the orbital layer and the bulbar layer, and the bulbar muscle fibers pass through the orbital layer.
【學(xué)位授予單位】：天津醫(yī)科大學(xué)
【學(xué)位級別】：博士
【學(xué)位授予年份】：2006
【分類號】：R322

【引證文獻】

相關(guān)碩士學(xué)位論文 前1條

1 王偉軍;毛竹稈莖維管束細胞壁結(jié)構(gòu)圖像分割與可視化研究[D];南京林業(yè)大學(xué);2007年

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本文編號：1714045