本文選題：胰島　切入點(diǎn)：導(dǎo)管細(xì)胞　出處：《重慶醫(yī)科大學(xué)》2005年博士論文

【摘要】：目 的: 1 型糖尿病的發(fā)生是由于胰島素的絕對(duì)缺乏,需終身依賴(lài)胰島素治療,但是胰島素治療并不能完全阻止糖尿病并發(fā)癥的發(fā)生,因此尋找合適的細(xì)胞以替代β細(xì)胞發(fā)揮生理功能具有重要的意義。Shapiro 等報(bào)道,給 7 例 1 型糖尿病患者移植新鮮胰島并配合非糖皮質(zhì)激素免疫抑制劑治療,患者不依賴(lài)外源胰島素而血糖控制良好的平均時(shí)間超過(guò)了 12 個(gè)月,可是,供體來(lái)源的匱乏以及移植免疫排斥反應(yīng)極大地阻礙了該方法的推廣和應(yīng)用。體外培養(yǎng)干細(xì)胞使其定向分化為胰島樣細(xì)胞以替代患者體內(nèi)受損胰島的生理功能,可能是解決供體來(lái)源匱乏以及移植免疫排斥反應(yīng)有效方法之一。研究證實(shí)小鼠胰腺導(dǎo)管上皮細(xì)胞能夠分化為具有內(nèi)分泌功能的胰島樣細(xì)胞, 本研究采用細(xì)胞培養(yǎng)及誘導(dǎo)分化技術(shù),分離培養(yǎng)小鼠胰腺導(dǎo)管上皮細(xì)胞并使之轉(zhuǎn)分化為胰腺干細(xì)胞及胰島素分泌細(xì)胞(胰島樣細(xì)胞),并將胰島樣細(xì)胞移植入 1 型糖尿病小鼠體內(nèi),定期觀察血糖水平 2 個(gè)月,為胰島細(xì)胞移植治療糖尿病奠定實(shí)踐基礎(chǔ)。 材料與方法: 1、 小鼠胰腺導(dǎo)管上皮細(xì)胞原代培養(yǎng)、誘導(dǎo)分化及鑒定:實(shí)驗(yàn)用成年昆明小鼠,以Ⅴ型膠原酶消化加濾網(wǎng)過(guò)濾法,分離小鼠胰腺導(dǎo)管上皮細(xì)胞,用添加有多種生長(zhǎng)因子的 DMEM/F12 培養(yǎng)基培養(yǎng)并誘導(dǎo)分
[Abstract]:Objective:Type 1 diabetes occurs because of an absolute lack of insulin, which requires life-long insulin therapy, but insulin therapy does not completely prevent the occurrence of diabetic complications.Therefore, it is important to find suitable cells to substitute 尾 cells for physiological function. Shapiro et al reported that 7 patients with type 1 diabetes were transplanted with fresh pancreatic islets and treated with non-glucocorticoid immunosuppressant.The average time for patients to control their blood sugar well without exogenous insulin is more than 12 months. However, the lack of donor sources and allograft immune rejection have greatly hindered the development and application of this method.In vitro culture of stem cells to differentiate them into islet like cells to replace the physiological function of damaged islets in patients may be one of the effective methods to solve the shortage of donor sources and the transplantation of immune rejection.It was proved that the pancreatic ductal epithelial cells of mice could differentiate into islet like cells with endocrine function. In this study, the technique of cell culture and differentiation was used to induce the differentiation of pancreatic ductal epithelial cells.Pancreatic ductal epithelial cells were isolated and cultured and transformed into pancreatic stem cells and insulin secreting cells (islet like cells), and islet like cells were transplanted into type 1 diabetic mice. Blood glucose levels were observed regularly for 2 months.To lay a practical foundation for the treatment of diabetes mellitus by islet cell transplantation.Materials and methods:1. Primary culture, differentiation and identification of pancreatic ductal epithelial cells in mice. The pancreatic ductal epithelial cells were isolated by collagenase V digestion and filter filtration in adult Kunming mice.Culture and Induction of DMEM/F12 medium with multiple growth factors
【學(xué)位授予單位】：重慶醫(yī)科大學(xué)
【學(xué)位級(jí)別】：博士
【學(xué)位授予年份】：2005
【分類(lèi)號(hào)】：R329.2

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相關(guān)期刊論文 前2條

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本文編號(hào)：1700859