本文選題：硫酸葡聚糖　切入點：豬　出處：《華中科技大學(xué)》2007年碩士論文

【摘要】： 第一部分低分子量硫酸葡聚糖抑制人補(bǔ)體系統(tǒng)活性的研究 目的研究不同濃度分子量為5000的低分子量硫酸葡聚糖(DXS)對人補(bǔ)系統(tǒng)活性的抑制作用。 方法根據(jù)DXS的濃度不同分為5個實驗組,DXS濃度分別為0.1mg/ml, 0.5mg/ml, 1mg/ml, 2mg/ml和4mg/ml,不含DXS組作為對照。CH50法檢測含有不同終濃度DXS的人血清的補(bǔ)體活性,MTT比色法測定含不同終濃度DXS的人血清對豬內(nèi)皮細(xì)胞殺傷率,免疫熒光法檢測豬內(nèi)皮細(xì)胞表面補(bǔ)體C3和IgM、IgG抗體沉積。 結(jié)果DXS可以抑制人血清補(bǔ)體活性,抑制人血清對豬內(nèi)皮細(xì)胞的毒性作用,劑量越大,抑制率越高,細(xì)胞表面補(bǔ)體C3抗體沉積越少,IgM、IgG抗體沉積沒有明顯差異。 結(jié)論低分子量硫酸葡聚糖能有效抑制人補(bǔ)體系統(tǒng)活性 第二部分低分子量硫酸葡聚糖抑制人補(bǔ)體介導(dǎo)的異種移植超急性排斥反應(yīng)的研究 目的研究分子量為5000的低分子量硫酸葡聚糖(DXS)抑制人補(bǔ)體激活而抑制異種抑制超急性排斥反應(yīng)的發(fā)生。 方法建立balbc小鼠心臟體外灌注模型,用含DXS終濃度分別為0.1mg/ml(n=8),2.0mg/ml(n=9)的10%人血清灌注液進(jìn)行灌注,以不含DXS的人血清組為對照(n=7),研究低分子量硫酸葡聚糖抑制人補(bǔ)體激活從而抑制人補(bǔ)體介導(dǎo)的超急性排斥反應(yīng). 結(jié)果心臟搏動時間對照組(n=7),0.1mg/ml組(n=7)和2.0mg/ml組(n=7)分別為17±11min, 49±15min和123±19min,含DXS組心臟搏動時間明顯長于對照組(Dunnett's T檢驗,P0.01);免疫熒光及免疫酶染色顯示2.0mg/ml組心臟內(nèi)補(bǔ)體C3c,C5b-9沉積較對照組明顯減少。 結(jié)論低分子量硫酸葡聚糖能有效抑制人補(bǔ)體激活從而抑制補(bǔ)體介導(dǎo)的超急性排斥反應(yīng)發(fā)生。
[Abstract]:A study on the inhibition of human complement system activity by low molecular weight dextran sulfate. Objective to study the inhibitory effect of low molecular weight dextran sulfate (DXS) with different concentration of 5000 on the activity of human supplement system. Methods according to the concentration of DXS, five experimental groups were divided into five groups: 0.1 mg / ml, 0.5 mg / ml, 1 mg / ml, 1 mg / ml, 2mg/ml and 4 mg / ml, respectively. The complement activity of human serum containing different final concentrations of DXS was determined by colorimetric method without DXS. The killing rate of human serum of DXS on porcine endothelial cells, The antibody deposition of complement C 3 and IgG on the surface of porcine endothelial cells was detected by immunofluorescence method. Results DXS could inhibit the complement activity of human serum and the toxicity of human serum to porcine endothelial cells. The higher the dose, the higher the inhibition rate, and the less C3 antibody deposition on the cell surface had no significant difference. Conclusion low molecular weight dextran sulfate can effectively inhibit the activity of human complement system. The second part of the study on suppressive effect of low molecular weight dextran sulfate on human complement mediated xenograft hyperacute rejection. Objective to study the effect of low molecular weight dextran sulfate (DXS) with molecular weight of 5000 on human complement activation and xenogeneic inhibition of hyperacute rejection. Methods the heart of balbc mice was perfused in vitro with 10% human serum perfused with 0.1 mg / ml of DXS final concentration of 2. 0 mg / ml of DXS. Low molecular weight dextran sulfate inhibited the activation of human complement and inhibited human complement mediated hyperacute rejection. Results the cardiac beating time in the control group was 17 鹵11 min, 49 鹵15min and 123 鹵19 min in the 2.0mg/ml group and 0.1 mg / ml group, respectively. The cardiac beating time in the DXS group was significantly longer than that in the control group (P 0.01), and immunofluorescence and immunoenzyme staining showed that the complement C5b-9 deposition in the heart of the 2.0mg/ml group was significantly longer than that in the 2.0mg/ml group. The control group was significantly decreased. Conclusion low molecular weight dextran sulfate can effectively inhibit the activation of human complement and inhibit the development of complement mediated hyperacute rejection.
【學(xué)位授予單位】：華中科技大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2007
【分類號】：R96;R392

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