本文選題：c-erbB_2　切入點(diǎn)：卵母細(xì)胞　出處：《江西醫(yī)學(xué)院》2005年碩士論文

【摘要】：目的和方法:卵母細(xì)胞成熟的機(jī)理尚不明了。本研究建立小鼠卵母細(xì)胞體外培養(yǎng)模型,用不同濃度反義c-erbB_2寡脫氧核苷酸(c-erbB_2ASODN)與未成熟卵母細(xì)胞共孵育,觀察其對(duì)卵母細(xì)胞體外成熟的影響;觀察表皮生長(zhǎng)因子(EGF)對(duì)卵母細(xì)胞體外成熟的作用以及c-erbB_2 ASODN 對(duì)EGF 促卵母細(xì)胞成熟的影響;利用RT-PCR 技術(shù)檢測(cè)卵母細(xì)胞中c-erbB_2mRNA 表達(dá)及在EGF、c-erbB_2ASODN 等不同處理因素作用下卵母細(xì)胞成熟過(guò)程中c-erbB_2 mRNA 表達(dá)的變化。為進(jìn)一步了解卵子的發(fā)育、成熟機(jī)理及其調(diào)控增添新資料,為開(kāi)辟新的促生育和抗生育途徑提供實(shí)驗(yàn)依據(jù)。 結(jié)果:(1)c-erbB_2ASODN 能呈劑量依賴方式抑制卵母細(xì)胞的GVBD 率和PBⅠ率,并顯著延遲其成熟的時(shí)間(低、中、高c-erbB_2ASODN 組24hGVBD 率比對(duì)照組分別低8.9%(P0.05)、26.6%(P0.01)和37.0%(P0.01);中、高c-erbB_2ASODN 組PBⅠ率比對(duì)照組分別低13.1%(P0.05)和22.7%(P0.01))。(2)EGF 可直接刺激裸卵的成熟分裂,并與其劑量和作用時(shí)間相關(guān)(1ng/mlEGF 組、10ng/mlEGF 組和50ng/mlEGF 組在24h 時(shí)的GVBD率比對(duì)照組分別高19.0%(P0.05)、27.0%(P0.01)20.6%(P0.05), PBⅠ率比對(duì)照組分別高13.0%、21.9%和14.4%(P0.05))。(3)c-erbB_2ASODN能抑制EGF 對(duì)卵母細(xì)胞的促成熟作用(EGF+ c-erbB_2ASON 組與EGF 組相比
[Abstract]:Objective and methods: the mechanism of oocyte maturation is not clear. In this study, mouse oocytes were cultured in vitro with different concentrations of antisense c-erbB_2 oligodeoxynucleotides c-erbB 2ASODN and immature oocytes. The effect of EGF on oocyte maturation in vitro, the effect of EGF on oocyte maturation in vitro and the effect of c-erbB_2 ASODN on oocyte maturation induced by EGF were observed. RT-PCR technique was used to detect the expression of c-erbB_2mRNA in oocytes and the changes of c-erbB_2 mRNA expression during oocyte maturation under the action of EGFC-erbB2ASODN, etc. In order to further understand the oocyte development, the mechanism of maturation and its regulation, new information was added. In order to open up a new procreation and antifertility pathway to provide experimental basis. Results c-erbB2ASODN inhibited the GVBD rate and PB 鈪，

本文編號(hào)：1686890