本文選題：水皰性口炎病毒　切入點(diǎn)：反向遺傳　出處：《吉林大學(xué)》2006年博士論文

【摘要】：隨著RNA病毒反向遺傳技術(shù)的進(jìn)展,水皰性口炎病毒(vesivular stomatitisvirus,VSV)載體已被開發(fā)成為一種有效的治療制劑。VSV病毒載體是一種高效的溶瘤病毒載體,具有非常廣的溶瘤范圍。資料報道VSV載體幾乎能夠溶解所有的瘤細(xì)胞,在體外實驗中VSV載體的溶瘤率都在50%以上,在體內(nèi)實驗中VSV載體能夠顯著延長荷瘤動物模型的壽命。VSV載體也被開發(fā)成為一種有效的疫苗載體, VSV作為疫苗載體應(yīng)用到獲得性免疫綜合癥病毒、流感病毒、丙型肝炎病毒和乙肝病毒等疫苗的研制過程中。因此水皰性口炎病毒載體具有非常好的應(yīng)用前景。 本實驗克隆了水皰性口炎病毒的全長cDNA序列(11161bp)以及L蛋白基因序列(6330bp)、N蛋白基因序列(1269bp)和P蛋白基因序列(798bp),并構(gòu)建了水皰性口炎病毒的反向遺傳重組系統(tǒng),即:包含VSV全長cDNA序列的反向遺傳重組載體和L蛋白、N蛋白和P蛋白的表達(dá)載體,并將這四種質(zhì)粒共同轉(zhuǎn)染BHK-21細(xì)胞,對VSV的反向遺傳重組進(jìn)行探索。這為水皰性口炎病毒載體的進(jìn)一步應(yīng)用打下基礎(chǔ)。
[Abstract]:With the development of reverse genetic technology of RNA virus, vesicular stomatitis virus (VSVV) vector has been developed as an effective therapeutic agent. It is reported that VSV vector can dissolve almost all tumor cells in vitro, and the rate of VSV vector is more than 50% in vitro. In vivo experiments, VSV vectors can significantly prolong the life span of tumor-bearing animal models. VSV vectors have also been developed as an effective vaccine vector. VSV is used as a vaccine vector for acquired immune syndrome virus, influenza virus. In the development of hepatitis C virus and hepatitis B virus vaccine, the vesicular stomatitis virus vector has a very good application prospect. In this study, we cloned the full-length cDNA sequence of vesicular stomatitis virus (BVV) 11161bp), the L protein gene sequence (63330bpHN) and the sequence of P protein gene (798 BP), and constructed the reverse genetic recombination system of blistering stomatitis virus (BMDV). That is, the reverse genetic recombinant vector containing the full-length cDNA sequence of VSV and the expression vector of L protein N protein and P protein were co-transfected into BHK-21 cells. The reverse genetic recombination of VSV was explored, which laid a foundation for the further application of vesicular stomatitis virus vector.
【學(xué)位授予單位】：吉林大學(xué)
【學(xué)位級別】：博士
【學(xué)位授予年份】：2006
【分類號】：Q78;R392

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相關(guān)期刊論文 前2條

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本文編號：1684526