本文選題：靜息軟骨細(xì)胞　切入點(diǎn)：刀豆蛋白A　出處：《吉林大學(xué)》2006年碩士論文

【摘要】：變形性關(guān)節(jié)炎等退形性關(guān)節(jié)疾病和關(guān)節(jié)機(jī)械性損傷的發(fā)病率在逐年提高。由于軟骨組織結(jié)構(gòu)的特殊性,缺乏血管,淋巴,神經(jīng)系統(tǒng)的營(yíng)養(yǎng)供給,給得軟骨疾病的治療恢復(fù)帶來(lái)了很大的困難。應(yīng)用組織工程學(xué)技術(shù)體外構(gòu)建人工軟骨,嘗試關(guān)節(jié)軟骨損傷的修復(fù)是國(guó)際上該領(lǐng)域的研究熱點(diǎn),軟骨細(xì)胞也是最早應(yīng)用于組織工程的種子細(xì)胞之一,但由于軟骨細(xì)胞作為種子細(xì)胞,其來(lái)源困難,增殖能力和數(shù)量有限,體外長(zhǎng)期培養(yǎng)的軟骨細(xì)胞易發(fā)生反分化現(xiàn)象,失去軟骨細(xì)胞的生物活性,限制了其應(yīng)用。因此對(duì)軟骨的增殖分化機(jī)制的研究,尋找強(qiáng)效的刺激因子必然對(duì)軟骨組織工程的發(fā)展,軟骨疾病的防治帶來(lái)很大的幫助。 刀豆球蛋白(Concanavalin A,簡(jiǎn)稱(chēng)ConA)是Sumner 1919年首次從大刀豆中分離純化出來(lái)的一種植物凝集素,后來(lái)Sumner和Howell[19]發(fā)現(xiàn)純化的ConA不僅能凝集紅細(xì)胞,而且還能沉淀溶液中的糖元和淀粉。隨著ConA的研究的深入,ConA的生物活性也逐漸得到認(rèn)識(shí),ConA對(duì)淋巴細(xì)胞具有強(qiáng)烈的促有絲分裂作用,刺激
[Abstract]:The incidence of degenerative joint diseases and mechanical joint injuries such as deformable arthritis is increasing year by year. It is very difficult to treat and recover the cartilage diseases. It is a hot spot in the world to construct artificial cartilage in vitro and try to repair the articular cartilage injury by using tissue engineering technology. Chondrocytes are also one of the first seed cells used in tissue engineering. However, because of the difficulty of chondrocytes as seed cells, their proliferation ability and quantity are limited, the long-term cultured chondrocytes are prone to dedifferentiation. The loss of the biological activity of chondrocytes limits its application. Therefore, the study on the mechanism of cartilage proliferation and differentiation and the search for strong stimulating factors are bound to be of great help to the development of cartilage tissue engineering and the prevention and treatment of cartilage diseases. Concanavalin A (Cona) is the first plant agglutinin isolated from concanavalin by Sumner in 1919. Later, Sumner and Howell [19] found that the purified ConA not only agglutinated red blood cells, Along with the further study of ConA, the biological activity of Cona has been gradually recognized that Cona has a strong mitogenic effect on lymphocytes and stimulates it.
【學(xué)位授予單位】：吉林大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2006
【分類(lèi)號(hào)】：R341;R684

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