本文選題：多器官功能障礙綜合征　切入點(diǎn)：間質(zhì)樹突狀細(xì)胞　出處：《中國人民解放軍軍醫(yī)進(jìn)修學(xué)院》2006年碩士論文

【摘要】：目的:觀察小鼠肺臟、肝臟與腎臟間質(zhì)樹突狀細(xì)胞的形態(tài)、數(shù)量和分布,了解MODS小鼠肺臟、肝臟與腎臟間質(zhì)樹突狀細(xì)胞病變以及DC功能相關(guān)的細(xì)胞因子、趨化因子及炎癥因子在MODS病程中的變化規(guī)律與意義,探討間質(zhì)DC在免疫紊亂、過度炎癥及臟器損傷機(jī)制中的影響與作用,為MODS免疫學(xué)機(jī)制與防治研究提供新的依據(jù)。 方法:腹腔注射酵母多糖復(fù)制C57BL/6小鼠MODS模型,分為正常對(duì)照、3-6小時(shí)、12-48小時(shí)、5-7日和10-12日組。光鏡與電鏡觀察MODS各期小鼠肺臟、肝臟和腎臟組織病理學(xué)改變,臟器間質(zhì)樹突狀細(xì)胞的形態(tài)、數(shù)量和分布特點(diǎn)及變化規(guī)律。運(yùn)用免疫學(xué)方法檢測(cè)間質(zhì)樹突狀細(xì)胞表面標(biāo)記物CD11c和CD205、共刺激分子CD80、CD86和MHC-Ⅱ類分子的表達(dá)水平及肺臟中IL-1β、IL-10及CGRP的表達(dá)情況。運(yùn)用RT-PCR法檢測(cè)趨化因子SLC及其受體CCR7在肺臟中的表達(dá)變化情況,判定MODS各期肺間質(zhì)樹突狀細(xì)胞的遷移活性。流式細(xì)胞術(shù)檢測(cè)MODS各期外周血CD4~+T細(xì)胞與CD8~+T細(xì)胞的數(shù)量與比值,外周血單個(gè)核細(xì)胞對(duì)MHC-Ⅱ分子表達(dá)的變化。 結(jié)果:間質(zhì)樹突狀細(xì)胞廣泛分布于正常小鼠肺臟、肝臟與腎臟中,數(shù)量極少,約占各臟器總細(xì)胞數(shù)的5-9‰,大部分處于非成熟狀態(tài),低表達(dá)CD80、CD86與MHC-Ⅱ分子。致傷早期(3-6小時(shí)),各臟器功能與病理改變輕微。間質(zhì)DC顯著增生,對(duì)CD80、CD86和MHC-Ⅱ分子的表達(dá)輕度增加。外周血中MHC-Ⅱ陽性的單個(gè)核細(xì)胞與CD4~+T細(xì)胞數(shù)均減少,CD4/8比值下降。趨化因子SLC及其受體CCR7在肺組織中表達(dá)水平均較正常組輕度上升。炎癥因子IL-1β、IL-10和CGRP在肺組織中陽性細(xì)胞數(shù)較正常組顯著升高;急性損傷期(12-48小時(shí)),各臟器出現(xiàn)功能改變,臟器實(shí)質(zhì)細(xì)胞損傷較重。間質(zhì)DC繼續(xù)保持增生狀態(tài),數(shù)量略低于致傷早期,但CD80、CD86和MHC-Ⅱ
[Abstract]:Objective: to observe the morphology, quantity and distribution of interstitial dendritic cells in lung, liver and kidney of mice, and to understand the cytokines related to the function of DC in lung, liver and kidney of MODS mice. The changes of chemokines and inflammatory factors in the course of MODS were studied. The effects of interstitial DC on immune disorder, excessive inflammation and organ injury were discussed, which provided a new basis for the study of immunological mechanism and prevention and treatment of MODS. Methods: the MODS model of C57BL/6 mice was induced by intraperitoneal injection of yeast polysaccharide. The mice were divided into normal control group (3-6 hours, 12-48 hours, 5-7 days and 10-12 days). The histopathological changes of lung, liver and kidney of MODS mice were observed by light and electron microscopy. The morphology of the mesenchymal dendritic cells, The quantity, distribution and changes of CD11c and CD205, the expression levels of CD80, CD86 and MHC- 鈪，

本文編號(hào)：1656869