本文選題：人胚心　切入點：發(fā)生　出處：《四川大學》2006年碩士論文　論文類型：學位論文

【摘要】：目的：血管內皮生長因子(vascular endothelial growth factor, VEGF)家族是由細胞分泌的多肽及蛋白質類化學信息分子，也是血管生成刺激因子中最重要的一類。許多研究證實新血管的生成和發(fā)育與VEGF家族有密切聯(lián)系。本實驗第一次采用人胚做材料，研究人胚心發(fā)生、發(fā)育的過程以及VEGF-A、VEGF-C、VEGFR-1、VEGFR-2、VEGFR-3在第3～8周人胚心內皮細胞、成心肌細胞中的表達情況，以進一步探討VEGF-A和VEGF-C在人早期心發(fā)生、發(fā)育過程中所起的作用。 材料及方法：收集意外流產、受精齡為3個月內的新鮮人胚。固定、包埋、切片、觀察，，根據(jù)胚層形成情況、體節(jié)的數(shù)目及器官發(fā)育狀況挑選胚齡為3～8w的標本切片。采用HE染色觀察早期心的外形及心內皮細胞、成心肌細胞的形態(tài)學變化；免疫組化對VEGF-A和VEGF-C及受體VEGFR-1、VEGFR-2、VEGFR-3在心內皮細胞及成心肌細胞的表達情況進行研究。 結果：HE染色結果顯示人胚第18、19天，臟壁中胚層內的成血管細胞局部增生形成生心區(qū)(cardiogenic area)。第3周末，形成縱行排列的內
[Abstract]:Objective: vascular endothelial growth factor (VEGF) family is a family of polypeptide and protein molecules secreted by cells. It is also one of the most important angiogenic stimulators. Many studies have shown that the formation and development of new blood vessels are closely related to the VEGF family. The process of development and the expression of VEGFR-1 and VEGFR-2VEGFR-3 in human embryonic heart endothelial cells and cardiomyocytes at the 3rd week were discussed in order to further explore the role of VEGF-A and VEGF-C in the early development of human heart. Materials and methods: fresh human embryos with accidental abortion and fertilization age within 3 months were collected, fixed, embedded, sectioned, observed, and based on the formation of the embryo. The number of somatic ganglia and organ development were selected for 8 weeks of embryo. The morphological changes of cardiac endothelial cells and cardiomyocytes were observed by HE staining. The expression of VEGF-A and VEGF-C and VEGFR-2 and VEGFR-3 in cardiac endothelial cells and cardiomyoblasts were studied by immunohistochemistry. Results on the 1819 th day of human embryo, the vascular cells in the mesoderm of the visceral wall were locally proliferated to form cardiogenic area cardiogenic area. At the end of the third week, they formed a longitudinal arrangement.
【學位授予單位】：四川大學
【學位級別】：碩士
【學位授予年份】：2006
【分類號】：R322

【參考文獻】

相關期刊論文 前2條

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2 梁魯南;血管內皮生長因子的研究進展[J];國外醫(yī)學(臨床生物化學與檢驗學分冊);2002年04期

本文編號：1631991