本文選題：人胚心　切入點：發(fā)生　出處：《四川大學(xué)》2006年碩士論文　論文類型：學(xué)位論文

【摘要】：目的：血管內(nèi)皮生長因子(vascular endothelial growth factor, VEGF)家族是由細(xì)胞分泌的多肽及蛋白質(zhì)類化學(xué)信息分子，也是血管生成刺激因子中最重要的一類。許多研究證實新血管的生成和發(fā)育與VEGF家族有密切聯(lián)系。本實驗第一次采用人胚做材料，研究人胚心發(fā)生、發(fā)育的過程以及VEGF-A、VEGF-C、VEGFR-1、VEGFR-2、VEGFR-3在第3～8周人胚心內(nèi)皮細(xì)胞、成心肌細(xì)胞中的表達(dá)情況，以進(jìn)一步探討VEGF-A和VEGF-C在人早期心發(fā)生、發(fā)育過程中所起的作用。 材料及方法：收集意外流產(chǎn)、受精齡為3個月內(nèi)的新鮮人胚。固定、包埋、切片、觀察，，根據(jù)胚層形成情況、體節(jié)的數(shù)目及器官發(fā)育狀況挑選胚齡為3～8w的標(biāo)本切片。采用HE染色觀察早期心的外形及心內(nèi)皮細(xì)胞、成心肌細(xì)胞的形態(tài)學(xué)變化；免疫組化對VEGF-A和VEGF-C及受體VEGFR-1、VEGFR-2、VEGFR-3在心內(nèi)皮細(xì)胞及成心肌細(xì)胞的表達(dá)情況進(jìn)行研究。 結(jié)果：HE染色結(jié)果顯示人胚第18、19天，臟壁中胚層內(nèi)的成血管細(xì)胞局部增生形成生心區(qū)(cardiogenic area)。第3周末，形成縱行排列的內(nèi)
[Abstract]:Objective: vascular endothelial growth factor (VEGF) family is a family of polypeptide and protein molecules secreted by cells. It is also one of the most important angiogenic stimulators. Many studies have shown that the formation and development of new blood vessels are closely related to the VEGF family. The process of development and the expression of VEGFR-1 and VEGFR-2VEGFR-3 in human embryonic heart endothelial cells and cardiomyocytes at the 3rd week were discussed in order to further explore the role of VEGF-A and VEGF-C in the early development of human heart. Materials and methods: fresh human embryos with accidental abortion and fertilization age within 3 months were collected, fixed, embedded, sectioned, observed, and based on the formation of the embryo. The number of somatic ganglia and organ development were selected for 8 weeks of embryo. The morphological changes of cardiac endothelial cells and cardiomyocytes were observed by HE staining. The expression of VEGF-A and VEGF-C and VEGFR-2 and VEGFR-3 in cardiac endothelial cells and cardiomyoblasts were studied by immunohistochemistry. Results on the 1819 th day of human embryo, the vascular cells in the mesoderm of the visceral wall were locally proliferated to form cardiogenic area cardiogenic area. At the end of the third week, they formed a longitudinal arrangement.
【學(xué)位授予單位】：四川大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2006
【分類號】：R322

【參考文獻(xiàn)】

相關(guān)期刊論文 前2條

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2 梁魯南;血管內(nèi)皮生長因子的研究進(jìn)展[J];國外醫(yī)學(xué)(臨床生物化學(xué)與檢驗學(xué)分冊);2002年04期

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