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  本文選題：大鼠　切入點：肢體　出處：《河北醫(yī)科大學(xué)》2005年碩士論文　論文類型：學(xué)位論文

【摘要】：肢體缺血-再灌注(I-R)是肢體擠壓傷、斷肢再植術(shù)及腹主動脈瘤手術(shù)等患者走向康復(fù)的病理轉(zhuǎn)歸過程,肢體嚴重缺血后再灌注可引發(fā)多器官功能衰竭(MOF),MOF 是此類患者最嚴重的并發(fā)癥,其死亡率占重癥監(jiān)護患者死亡率的30-40%。因此,探討肢體I-R 引發(fā)多器官損傷的機制,一直是創(chuàng)傷外科及重癥醫(yī)學(xué)共同關(guān)注的重大課題。 既往的研究多集中在腎、肺等臟器上,本課題組的研究則發(fā)現(xiàn),大鼠肢體I-R 后其海馬區(qū)錐體層神經(jīng)元也出現(xiàn)明顯的病理改變。海馬是與學(xué)習(xí)、記憶及免疫調(diào)節(jié)密切相關(guān)的中樞部位,其病損可能是外周創(chuàng)傷導(dǎo)致記憶缺失及免疫功能低下的重要原因。因此,闡明肢體I-R 引發(fā)海馬神經(jīng)元損傷的機制,具有重要的理論意義和臨床應(yīng)用價值。 Fas 蛋白是腫瘤壞死因子受體家族成員,受其配體Fasl激活后可啟動細胞的凋亡程序。在正常腦的海馬區(qū),Fas 蛋白僅維持極低的表達水平,但腦I-R 后海馬區(qū)神經(jīng)元Fas 蛋白的表達水平明顯上調(diào)。肢體I-R 所致腦損傷與腦自身I-R損傷的機制不同,Fas 蛋白是否參與介導(dǎo)了肢體I-R 后的海馬損傷,尚無研究證實。本實驗擬應(yīng)用免疫組化染色方法,觀測大鼠肢體I-R 后海馬區(qū)Fas 蛋白表達的時空變化規(guī)律,為最終闡明肢體I-R 引發(fā)腦損傷的機制積累資料。 目的:通過觀測大鼠肢體缺血再灌注后海馬神經(jīng)元Fas蛋白表達的變化,證明Fas 蛋白是否介入了肢體I-R 所致腦
[Abstract]:Limb ischemia-reperfusion (I-R) is the pathological process of limb compression injury, amputated limb replantation and abdominal aortic aneurysm surgery. MOF / MOF is the most serious complication in patients with severe limb ischemia and reperfusion. The mortality rate is 30-400.Therefore, the mechanism of limb I-R induced multiple organ injury is discussed. Trauma surgery and critical medicine have been a major issue of common concern. Previous studies have focused on the kidneys, lungs and other organs. Our team found that the hippocampal pyramidal neurons also showed pathological changes after I-R. The lesion of central part closely related to memory and immune regulation may be an important cause of memory loss and low immune function caused by peripheral trauma. Therefore, the mechanism of hippocampal neuron injury induced by limb I-R is clarified. It has important theoretical significance and clinical application value. Fas protein is a member of the tumor necrosis factor receptor family and activated by its ligand Fasl can initiate the apoptosis process. However, the expression of Fas protein in hippocampal neurons after I-R was significantly up-regulated. The mechanism of brain injury induced by limb I-R is different from that of brain I-R. Whether FAS protein is involved in the hippocampal injury induced by I-R in limbs. The present study intends to observe the temporal and spatial changes of Fas protein expression in hippocampal region of rats after limb I-R by immunohistochemical staining, in order to clarify the mechanism of brain injury induced by limb I-R. Objective: to observe the changes of Fas protein expression in hippocampal neurons after limb ischemia-reperfusion in rats, and to prove whether Fas protein is involved in the brain induced by limb I-R.
【學(xué)位授予單位】：河北醫(yī)科大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2005
【分類號】：R361

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本文編號：1627297