本文選題：流行性感冒病毒　切入點(diǎn)：Vero細(xì)胞　出處：《吉林大學(xué)》2007年碩士論文　論文類(lèi)型：學(xué)位論文

【摘要】： 流感是由甲(A)、乙(B)、丙(C)三型流感病毒分別引起的一種急性呼吸道傳染病。甲、乙、丙不僅反映了病毒被發(fā)現(xiàn)的年代及前后順序,更主要的是反映了對(duì)人類(lèi)危害程度的順序。其重要性在于可引起嚴(yán)重的發(fā)病率和死亡率,影響學(xué)校、公共服務(wù)機(jī)構(gòu)和各單位的正常工作。超額死亡率也與流感有關(guān),死亡者多為患有慢性疾病和年邁體衰的老年人。目前唯一可行的流感預(yù)防措施是疫苗接種。 自1941年流感滅活疫苗在美國(guó)首次批準(zhǔn)使用以來(lái),流感疫苗已使用60多年。在此期間,廣泛的經(jīng)驗(yàn)已證明疫苗的安全性和效力。每年在流感流行季節(jié)前在流感的高危人群中進(jìn)行預(yù)防接種是減輕流感危害的重要措施。疫苗接種不僅可以降低高危人群的發(fā)病率和死亡率,也可減輕癥狀,降低并發(fā)癥的發(fā)生,降低繼續(xù)傳播的機(jī)率。 目前研究應(yīng)用于滅活流感疫苗生產(chǎn)的細(xì)胞系主要有MDCK、VERO、PER.C6三個(gè)細(xì)胞系。其中Vero傳代細(xì)胞系解決了外源病毒和致癌性的問(wèn)題,且易于大規(guī)模培養(yǎng),被WHO推薦作為疫苗生產(chǎn)細(xì)胞基質(zhì)之一,已成功開(kāi)發(fā)出了狂犬病、脊髓灰質(zhì)炎、流行性乙腦炎等疫苗。使用哺乳動(dòng)物細(xì)胞培養(yǎng)技術(shù)替代雞胚來(lái)生產(chǎn)流感疫苗將是大勢(shì)所趨。 基于上述情況,本文作者通過(guò)廣泛的文獻(xiàn)檢索與反復(fù)試驗(yàn),現(xiàn)已完成各(亞)型流感病毒于Vero細(xì)胞內(nèi)的快速傳代工藝,建立了流感病毒滅活疫苗(Vero細(xì)胞)生產(chǎn)用病毒種子批系統(tǒng),解決了流感病毒于細(xì)胞內(nèi)傳代穩(wěn)定性問(wèn)題,為進(jìn)一步研制流感病毒滅活疫苗(Vero細(xì)胞)打下了良好的基礎(chǔ)。
[Abstract]:Influenza is an acute respiratory infectious disease caused by A, B, C) influenza viruses. A, B, C not only reflect the date and sequence of the virus being discovered. More importantly, it reflects the order of harm to human beings. Its importance lies in causing serious morbidity and mortality, affecting the normal work of schools, public services and units. Excess mortality is also associated with influenza. Most of the deaths are among the elderly with chronic illness and frailty. Vaccination is the only available influenza prevention measure. The flu vaccine has been in use for more than 60 years since 1941, when it was first approved for use in the United States. Extensive experience has proved the safety and effectiveness of vaccines. Vaccination every year before the influenza epidemic season in the influenza high-risk population is an important measure to reduce the risk of influenza. Vaccination can not only reduce the high-risk population. Morbidity and mortality, Can also reduce symptoms, reduce the incidence of complications, reduce the probability of further transmission. At present, the main cell lines used in the production of inactivated influenza vaccine are MDCK VEROPER.C6. Among them, Vero subculture cell line solves the problems of exogenous virus and carcinogenicity, and is easy to be cultured on a large scale. Rabies, poliomyelitis and Japanese encephalitis have been successfully developed as one of the cell matrix for vaccine production by WHO. It is a general trend to use mammalian cell culture technology to replace chicken embryo to produce influenza vaccine. In view of the above situation, the author has completed the rapid passage of various (subtype) influenza viruses in Vero cells through extensive literature retrieval and repeated experiments. A virus seed batch system was established for the production of inactivated influenza virus vaccine (Vero cells), which solved the problem of intracellular transmission stability of influenza virus and laid a good foundation for the further development of inactivated influenza virus vaccine Vero cells.
【學(xué)位授予單位】：吉林大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2007
【分類(lèi)號(hào)】：R392

【共引文獻(xiàn)】

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1 黃錠;趙亮;譚文松;;犬腎細(xì)胞MDCK無(wú)血清貼壁及單細(xì)胞懸浮培養(yǎng)[J];生物工程學(xué)報(bào);2011年04期

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相關(guān)碩士學(xué)位論文 前6條

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5 黃錠;用于流感疫苗生產(chǎn)的MDCK細(xì)胞無(wú)血清單細(xì)胞懸浮培養(yǎng)體系的開(kāi)發(fā)[D];華東理工大學(xué);2010年

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