本文選題：壓瘡　切入點(diǎn)：缺血再灌注　出處：《護(hù)理學(xué)雜志》2014年15期 　論文類型：期刊論文

【摘要】：目的構(gòu)建大鼠深部組織損傷模型,觀察和評(píng)價(jià)該模型的病理特點(diǎn)及臨床意義。方法將25只雄性SD大鼠隨機(jī)分為1個(gè)正常組和4個(gè)實(shí)驗(yàn)組,利用磁片循環(huán)壓迫的方法,對(duì)4個(gè)實(shí)驗(yàn)組大鼠建立壓瘡深部組織損傷的動(dòng)物模型,并從整體、組織和生化3個(gè)層次對(duì)壓瘡深部組織潰瘍進(jìn)行觀察。結(jié)果正常組皮膚及肌肉組織肉眼及鏡下觀察結(jié)構(gòu)完整;實(shí)驗(yàn)組受壓組織初期出現(xiàn)輕度凹陷、水腫,但表面皮膚尚完整,隨著時(shí)間延長(zhǎng),至第7天起逐漸出現(xiàn)少許破潰現(xiàn)象;進(jìn)一步的病理切片觀察顯示皮膚、皮下、肌肉組織全層受損,各組均有大量炎癥細(xì)胞浸潤(rùn),復(fù)層鱗狀上皮變薄,膠原纖維排列紊亂,層次欠佳,肌纖維廣泛變性,橫紋結(jié)構(gòu)逐漸消失;鏡下觀察受壓早期,第0天肌肉組織即已受損,且據(jù)第14天組的觀察發(fā)現(xiàn)肌肉組織后期修復(fù)較皮膚緩慢;Tunel染色檢測(cè)皮膚及肌肉組織凋亡情況,各實(shí)驗(yàn)組(第0、4、7、14天)與正常對(duì)照組比較,差異有統(tǒng)計(jì)學(xué)意義(均P0.01)。結(jié)論利用磁片循環(huán)壓迫的方法構(gòu)建的壓瘡深部組織損傷動(dòng)物模型具有組織壞死、炎癥細(xì)胞聚集、細(xì)胞凋亡等特征,其病理變化符合壓瘡臨床病理特點(diǎn),且成模時(shí)間短,可控性強(qiáng),是一種理想的用于研究壓瘡機(jī)制的動(dòng)物模型。
[Abstract]:Objective to establish a rat model of deep tissue injury and to observe and evaluate the pathological characteristics and clinical significance of the model. Methods 25 male SD rats were randomly divided into 1 normal group and 4 experimental groups. The animal models of deep tissue injury were established in 4 experimental groups, and the ulcers of deep tissue were observed from the whole, tissue and biochemical levels. Results the skin and muscle tissues of normal group were observed with naked eye and microscope. In the experimental group, there was slight depression and edema at the initial stage of compression tissue, but the surface skin was still intact, and with the extension of time, a little burst appeared gradually on the 7th day, and further pathological examination showed that the skin, subcutaneous, subcutaneous, and subcutaneous. The whole layer of muscle tissue was damaged, there were a large number of inflammatory cells infiltrated in each group, the laminated squamous epithelium became thinner, the collagenous fibers were disordered, the layers were not good, the muscle fibers were extensively denatured, and the transverse structure gradually disappeared. On day 0, the muscle tissue was damaged. According to the observation of the 14th day group, it was found that the late repair of muscle tissue was slower than the Tunel staining of skin, and the apoptosis of skin and muscle tissue was detected by Tunel staining. Conclusion the animal model of deep tissue injury by magnetic disc circulation compression has the characteristics of tissue necrosis, inflammatory cell aggregation and apoptosis. The pathological changes of the animal model accord with the clinicopathological characteristics of pressure sore. It is an ideal animal model for studying the mechanism of pressure sore because of its short time and strong controllability.
【作者單位】： 溫州醫(yī)科大學(xué)護(hù)理學(xué)院;
【基金】：國(guó)家自然科學(xué)基金項(xiàng)目(81372064)
【分類號(hào)】：R632.1;R-332
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本文編號(hào)：1585659