本文選題：斯氏貍殖吸蟲　切入點：肝損害　出處：《昆明醫(yī)學(xué)院》2005年碩士論文　論文類型：學(xué)位論文

【摘要】：【目的】研究斯氏貍殖吸蟲對大鼠肝臟結(jié)構(gòu)和功能的損害情況;探討斯氏貍殖吸蟲引起大鼠肝臟損害的機制;評價肝功能檢查在斯氏貍殖吸蟲病肝臟損害的早期診斷中的價值。 【方法】 35只SD大鼠隨機分成3組:對照組、實驗A組、實驗B組,將斯氏貍殖吸蟲囊蚴經(jīng)腹腔注射感染實驗組大鼠以建立動物模型(每鼠注射15個囊蚴),用全自動生化分析儀于感染后不同時間分別檢測對照組和實驗A組大鼠血清總蛋白、白蛋白、球蛋白、丙氨酸氨基轉(zhuǎn)移酶(ALT)、天冬氨酸氨基轉(zhuǎn)移酶(AST)、血清總膽紅素、血清結(jié)合膽紅素與非結(jié)合膽紅素等肝功能指標(biāo),并進行統(tǒng)計分析;于感染后不同時間分別剖殺實驗B組的部分大鼠,用光學(xué)顯微鏡、透射電鏡觀察肝臟組織病理學(xué)變化。 【結(jié)果】斯氏貍殖吸蟲對大鼠肝臟造成明顯損害,肝臟功能損害主要表現(xiàn)為:感染后4、6、8周實驗A組大鼠血清白蛋白含量明顯下降,感染后4、6、8、52周血清球蛋白含量明顯升高,而總蛋白含量在感染后變化不明顯;感染后6、8周,ALT與AST活性均升高;血清總膽紅素、血清結(jié)合膽紅素與非結(jié)合膽紅素在感染后改變不明顯;肝功能各指標(biāo)在感染后的前2周變化不明顯。肝臟結(jié)構(gòu)損害主要表現(xiàn)為:感染后1周,肝細(xì)胞水腫,少數(shù)肝細(xì)胞脂肪變性;感染后2周,肝臟突出的病變?yōu)榧毙曰撔匝装Y,在肝組織內(nèi)形成小膿腫;感染后8周,肝組織內(nèi)出現(xiàn)片狀纖維組織增生,部分肝小葉結(jié)構(gòu)破壞、重建:感染后52周,肝臟纖維化改變明顯,有大量假小葉形成。透射電鏡觀察到:從感染后2周開始,實驗B組大鼠肝臟均呈現(xiàn)出不同程度的纖維化,8周以后纖維化明顯
[Abstract]:[objective] to study the damage of paragonimus skrjabini on the structure and function of rat liver, to explore the mechanism of hepatic damage induced by P. skrjabini, and to evaluate the value of liver function examination in the early diagnosis of hepatic damage caused by paragonimiasis skrjabini. [methods] Thirty-five Sprague-Dawley rats were randomly divided into three groups: control group, experimental group A, experimental group B. In order to establish the animal model, the rats of experimental group were infected with Cysticercus skrjabini by intraperitoneal injection. The serum total protein of control group and experimental group A were detected by automatic biochemical analyzer at different time after infection. Albumin, globulin, alanine aminotransferase (alt), aspartate aminotransferase (AST), serum total bilirubin, serum conjugated bilirubin and unconjugated bilirubin were analyzed statistically. Some rats in group B were killed at different time after infection. The histopathological changes of liver were observed by optical microscope and transmission electron microscope. [results] the liver of rats was obviously damaged by paragonimus skrjabini. The main damage of liver function was as follows: the serum albumin content of experimental group A decreased significantly, and the content of serum globulin increased significantly at 852 weeks after infection in group A. The serum total bilirubin, serum conjugated bilirubin and unconjugated bilirubin did not change significantly after infection. The changes of liver function in the first two weeks after infection were not obvious. The main structural damage of liver was: 1 week after infection, edema of liver cells, fatty degeneration of a few liver cells, and acute suppurative inflammation 2 weeks after infection. Small abscess was formed in the liver tissue, and at 8 weeks after infection, the flake fibrous tissue proliferated and some hepatic lobules were destroyed, and the liver fibrosis changed obviously at 52 weeks after infection. A large number of pseudolobules were formed. It was observed by transmission electron microscope that from 2 weeks after infection, the liver of experimental group B showed various degrees of fibrosis.
【學(xué)位授予單位】：昆明醫(yī)學(xué)院
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2005
【分類號】：R383

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