本文選題：臂旁核　切入點(diǎn)：中央杏仁核　出處：《南京醫(yī)科大學(xué)》2006年碩士論文　論文類型：學(xué)位論文

【摘要】：目的：1．觀察內(nèi)臟傷害性刺激后，大鼠臂旁核(PBN)內(nèi)被激活神經(jīng)元的亞核定位情況；2．探討PBN內(nèi)神經(jīng)元型一氧化氮合酶(nNOS)是否參與對(duì)內(nèi)臟傷害性刺激傳導(dǎo)過(guò)程的調(diào)節(jié)；3．探討臂旁核-中央杏仁核通路(PBN-CeA)是否參與對(duì)內(nèi)臟傷害性刺激的上傳過(guò)程，并闡明nNOS是否參與了對(duì)此傳導(dǎo)通路的調(diào)節(jié)。 方法：1．給予大鼠2％福爾馬林溶液灌胃后，用Fos蛋白免疫組織化學(xué)方法觀察PBN內(nèi)Fos陽(yáng)性神經(jīng)元在各亞核內(nèi)的分布情況；2．用還原型尼克酰胺腺嘌呤二核苷酸脫氫酶(NADPH-d)組織化學(xué)結(jié)合Fos蛋白免疫組織化學(xué)雙重染色的方法，觀察內(nèi)臟傷害性刺激后，F(xiàn)os陽(yáng)性神經(jīng)元和nNOS陽(yáng)性神經(jīng)元及纖維在PBN各亞核內(nèi)的分布與共存情況；3．大鼠CeA內(nèi)注射熒光金(FG)后，用FG、Fos免疫熒光結(jié)合NADPH-d組織化學(xué)三標(biāo)染色的方法，觀察內(nèi)臟傷害性刺激后Fos、FG和nNOS在PBN內(nèi)的分布及共存情況。 結(jié)果：1．同本實(shí)驗(yàn)室既往觀察結(jié)果類似，，在正常大鼠PBN內(nèi)具有NADPH-d的廣泛分布：TypeⅠ型NADPH-d陽(yáng)性神經(jīng)元顏色深染、突起較長(zhǎng)，主要分布在PBN的嘴側(cè)部分；TypeⅡ型NADPH-d陽(yáng)性神經(jīng)元染色較淡，突起較短，主要沿PBN的背外側(cè)界分布；PBN嘴側(cè)可見(jiàn)大量的近似水平方向走行的纖維束穿過(guò)小腦上腳(scp)，臂旁外側(cè)核外亞核(PBel)內(nèi)可見(jiàn)密集點(diǎn)狀分布的NADPH-d陽(yáng)性纖維終末。2．內(nèi)臟傷害性刺激后，F(xiàn)os陽(yáng)性神經(jīng)元在PBN內(nèi)的分布具有亞核特異性：PBel、K-F核和臂旁外側(cè)核背亞核(PBdl)有大量分布；臂旁外側(cè)核中央亞核有少量分布；其余各
[Abstract]:Objective: 1.After observing visceral nociceptive stimulation, Subnuclear localization of activated neurons in parabrachial nucleus of rats 2. To investigate the role of neuronal nitric oxide synthase (nNOS) in the conduction of visceral nociceptive stimulation in PBN. To explore the pathway of parabrachial nucleus to central amygdaloid nucleus (PBN-CeA). Whether or not to participate in the process of uploading visceral noxious stimuli, And whether nNOS is involved in the regulation of this transduction pathway. Methods 1. Rats were given 2% formalin solution. The distribution of Fos positive neurons in the subnuclei of PBN was observed by immunohistochemical method of Fos protein. The double staining method of NADPH-dH histochemistry combined with Fos protein was used to detect the distribution of Fos positive neurons in the subnuclei of PBN by using reduced nicotinamide adenine dinucleotide dehydrogenase (NADPH-d). To observe the distribution and coexistence of Fos positive neurons and nNOS positive neurons and fibers in the subnuclei of PBN after visceral nociceptive stimulation 3.After the rats were injected with FG into CeA, the FGS-Fos immunofluorescence and NADPH-d histochemical staining were used. After visceral nociceptive stimulation, the distribution and coexistence of Fosson-FG and nNOS in PBN were observed. Results (1) similar to the previous observations in our laboratory, there was a widespread distribution of NADPH-d in PBN of normal rats. The positive neurons of NADPH-d type 1 were dark stained and had long protrusions. The staining of type 鈪

本文編號(hào)：1557023