發(fā)布時(shí)間：2018-03-02 11:57

  本文選題：腺病毒　切入點(diǎn)：TCID　出處：《現(xiàn)代預(yù)防醫(yī)學(xué)》2014年23期 　論文類(lèi)型：期刊論文

【摘要】：目的建立腺病毒滴度測(cè)定的TCID50方法,并比較MDCK、Vero、HEp-2及A549 4種細(xì)胞對(duì)人呼吸道腺病毒的敏感性。方法將腺病毒作連續(xù)10倍稀釋,每個(gè)稀釋度取100μl加入96孔細(xì)胞培養(yǎng)板中,隨后加入消化的宿主細(xì)胞100μl,每個(gè)稀釋度作10個(gè)重復(fù),并設(shè)空白細(xì)胞培養(yǎng)對(duì)照。置37℃,5%CO2培養(yǎng)箱中。逐日觀察細(xì)胞病變,并記錄細(xì)胞病變孔數(shù),直到對(duì)照細(xì)胞老化脫落為止,按Reed-Muench法計(jì)算病毒的TCID50。結(jié)果呼吸道腺病毒可在MDCK、Vero、HEp-2及A549四種細(xì)胞中增殖,并出現(xiàn)典型細(xì)胞病變,在觀察期內(nèi)A549和Vero細(xì)胞能測(cè)出TCID50值。結(jié)論四種細(xì)胞對(duì)腺病毒的敏感性依次為A549VeroHep-2MDCK。
[Abstract]:Objective to establish a TCID50 method for the determination of adenovirus titer, and to compare the sensitivity of MDCKV Verohep-2 and A549 cells to human respiratory adenovirus. Methods Adenoviruses were diluted continuously for 10 times, and 100 渭 l per dilution was added to 96 well cell culture plate. Then 100 渭 l of digested host cells were added, each dilution was repeated 10 times, and blank cell culture was set up as control. The cytopathic cells were observed daily in 37 鈩，

本文編號(hào)：1556423