本文關(guān)鍵詞： 癲癇 顳葉/化學(xué)誘導(dǎo) 迷宮學(xué)習(xí) 記憶障礙/并發(fā)癥 鈣離子　出處：《安徽醫(yī)科大學(xué)》2006年碩士論文　論文類型：學(xué)位論文

【摘要】：目的：(1)探討利用立體定向方法建立大鼠顳葉癲癇模型的方法以及觀察發(fā)作后的腦電活動、MR表現(xiàn)和病理變化。(2)觀察大鼠顳葉癲癇發(fā)作后不同時間段學(xué)習(xí)記憶功能障礙的情況以及探討其與海馬病理變化、海馬神經(jīng)元內(nèi)游離鈣離子濃度變化的相關(guān)性。方法：選擇SD大鼠60只，隨機分組法分為正常對照組、注射生理鹽水組、注射紅藻氨酸誘發(fā)顳葉癲癇發(fā)作后2周組、1月組、2月組、3月組，每組各10只。注射紅藻氨酸組利用立體定向方法在大鼠右側(cè)海馬CA_3區(qū)注射紅藻氨酸以誘發(fā)顳葉癲癇發(fā)作，注射生理鹽水組在右側(cè)海馬CA_3區(qū)注射生理鹽水，注射后分別用視頻攝像機記錄、觀察大鼠行為學(xué)表現(xiàn)，定期在雙側(cè)海馬、額葉皮質(zhì)置入深部電極描記腦電變化。利用3.0T的MR對大鼠進(jìn)行MRI和MRS檢查。利用Morris水迷宮實驗評價各組大鼠學(xué)習(xí)記憶障礙程度，隨后處死大鼠進(jìn)行病理檢查，觀察海馬、額葉皮質(zhì)的病理變化與學(xué)習(xí)記憶功能障礙的相關(guān)性。利用鈣離子探針Fluo-3／AM，在激光掃描共聚焦顯微鏡下檢測不同時間段的海馬神經(jīng)元內(nèi)游離鈣離子濃度的變化。結(jié)果：正常對照組和注射生理鹽水組無顳葉癲癇發(fā)作和學(xué)習(xí)記憶障礙。右側(cè)海馬注射紅藻氨酸的各組在注射紅藻氨酸后均出現(xiàn)顳葉癲癇發(fā)作，腦電圖描記出起源于注射側(cè)海馬并向?qū)?cè)海馬和雙側(cè)額葉皮質(zhì)傳導(dǎo)的癲癇波。MR檢查提示注射紅藻氨酸側(cè)海馬出現(xiàn)長TI長T2信號病灶。水迷宮實驗中，有癲癇發(fā)作的各組出現(xiàn)不同程度的學(xué)習(xí)記憶功能障礙，定位航行實驗中逃避潛伏期延長，空間搜索實驗中在原有平臺象限內(nèi)游泳時間的百分比下降，與正常對照組和注射生理鹽水組比較均有顯著性差異(P＜0.05)，并隨發(fā)作時間的延長逐漸加重，發(fā)作后2月達(dá)到高峰。病理檢查發(fā)現(xiàn)，，注射紅藻氨酸后右側(cè)海馬錐體細(xì)胞逐漸出現(xiàn)變性壞死，由CA_3區(qū)向CA_2、CA_4、CA_1區(qū)擴展，隨后對側(cè)海馬錐體細(xì)胞也出現(xiàn)變性壞死，但程度明顯低于注射側(cè)，上述病理改變在注射后2月達(dá)到高峰，
[Abstract]:Objective: to explore the method of establishing rat temporal lobe epilepsy model by stereotactic method, and to observe the brain electrical activity and its pathological changes after seizure. 2) to observe the learning and memory impairment in different time periods after seizure of temporal lobe epilepsy in rats. The condition of obstruction and its relation to the pathological changes of the hippocampus, Methods: 60 SD rats were randomly divided into normal control group and saline injection group. Ten rats in each group were injected with kainic acid for 2 weeks, January, February and March, respectively. Kainic acid was injected into the CA_3 region of the right hippocampus of rats by stereotactic method to induce temporal lobe epilepsy. The rats in the saline group were injected with saline in the CA_3 area of the right hippocampus. The rats were recorded with video camera after injection, and the behavior of the rats was observed regularly in the bilateral hippocampus. MRI and MRS were examined by 3.0T Mr. The degree of learning and memory impairment was evaluated by Morris water maze test. Then the rats were sacrificed for pathological examination and hippocampal observation. The relationship between the pathological changes of frontal cortex and learning and memory impairment. Using calcium probe Fluo-3 / AM, the changes of free calcium concentration in hippocampal neurons at different periods of time were detected by laser scanning confocal microscope. There were no temporal lobe seizures and learning and memory disorders in normal control group and saline injection group. All the right hippocampal groups injected kainic acid had temporal lobe epilepsy after injection of kainic acid. EEG records of epileptic waves originating in the injected hippocampus and conducting to the contralateral hippocampus and bilateral frontal cortex suggest that the hippocampus injected with kainic acid presents a long TI long T2 signal lesion. Different degrees of learning and memory dysfunction appeared in each group with epileptic seizures, the latency of escape was prolonged, and the percentage of swimming time in the original platform quadrant decreased in the spatial search experiment. Compared with the normal control group and the saline injection group, there was significant difference (P < 0.05), and gradually aggravated with the prolongation of the attack time, and reached the peak on February after the attack. After injection of kainic acid, degeneration and necrosis of the right hippocampal pyramidal cells gradually appeared, which expanded from the CA_3 region to CA2CA4 and CA1 area, then the contralateral hippocampal pyramidal cells also showed degeneration and necrosis, but the degree was significantly lower than that of the injection side. The pathological changes peaked on February after injection.
【學(xué)位授予單位】：安徽醫(yī)科大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2006
【分類號】：R742.1;R-332

【引證文獻(xiàn)】

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