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  本文關鍵詞： 幽門螺桿菌 雙向凝膠電泳 基因缺失突變　出處：《山東大學》2005年碩士論文　論文類型：學位論文

【摘要】：幽門螺桿菌(Helicobacter pylori,H. pylori)作為唯一能在人體胃內酸性環(huán)境中定植并長期生存的致病菌,其生長在體內必然會受到各種不利條件的影響,H. pylori通過啟動一系列的適應性調節(jié)機制來抵抗這些脅迫因素以達到保種生存并導致疾病的目的。形態(tài)學變化是H. pylori的適應性調控機制之一,在不同的脅迫條件下H. pylori可發(fā)生不同的形態(tài)學變化,蛋白質是生命活動的最終執(zhí)行者,無論何種表征蛋白質在其中將起重要作用,比較蛋白質組學是研究病原菌在各種脅迫因素下蛋白質表達差異的理想工具,現(xiàn)今較普遍使用的技術體系包括雙向凝膠電泳,質譜分析及生物信息學分析。膽汁是機體重要的天然抗病原菌物質,研究H. pylori在膽汁中的存活能力,對于揭示H. pylori感染與十二指腸潰瘍的發(fā)生規(guī)律有重要意義,為此我們研究了H. pylori在膽汁脅迫下的蛋白表達狀態(tài)。絲狀體是H. pylori除螺桿狀和球形體以外的一種成活形式,對絲狀體蛋白表達譜的研究,有助于揭示其發(fā)生規(guī)律。我們曾發(fā)現(xiàn)鞭毛絲帽蛋白與H. pylori結構性群體結構形成密切相關,然而,對生物質譜篩選的候選蛋白尚需作進一步的功能驗證,為此,我們構建了H. pylori結構性群體形成相關蛋白鞭毛絲帽蛋白基因缺失突變株,為進一步驗證此蛋白的功能及闡明H. pylori結構性群體的形成機制奠定工作基礎。主要研究內容及研究結果如下: 一、膽汁對幽門螺桿菌的抑制及其蛋白質表達譜的初步研究 膽汁脅迫是人體內抑制H. pylori生長的不利條件之一,許多研究也表明膽汁能夠抑制H. pylori的生長,但H. pylori仍能夠在膽汁豐富的十二指腸球部存在,而對其他肝膽系統(tǒng)的感染較少見,因此我們推測膽汁的酸化影響其對H. pylori的抑制活性。為了研究H. pylori對膽汁脅迫的適應性調
[Abstract]:Helicobacter pylori Helicobacter pylori H.pylori is the only bacterium that can colonize and survive in an acidic environment in the stomach of a human body. Its growth in vivo is bound to be affected by various adverse conditions. H. pylori protects against these stress factors by initiating a series of adaptive regulatory mechanisms to preserve the survival of species and lead to disease. Morphological changes are H. pylori. One of the adaptive regulatory mechanisms, Under different stress conditions, the morphological changes of H. pylori are different. Protein is the final executor of life activity, no matter what characterization protein will play an important role in it. Comparative proteomics is an ideal tool for studying the differences in protein expression of pathogens under various stress factors. Bile is an important natural antipathogenic substance in the body. It is important to study the survival ability of H. pylori in bile for revealing the regularity of H. pylori infection and duodenal ulcer. In this paper, we studied the protein expression of H. pylori under bile stress. The filamentous body is a survival form of H. pylori except the screw and spheroids, and the protein expression profile of the filamentous body is studied. We have found that flagellum cap protein is closely related to the formation of structural population structure of H. pylori. However, the candidate proteins screened by mass spectrometry need further functional verification. We constructed a mutant with deletion of flagellin gene associated with the structural colony formation of H. pylori. In order to further verify the function of the protein and elucidate the formation mechanism of the structural population of H. pylori, the main contents and results are as follows:. A preliminary study on the inhibition of bile on Helicobacter pylori and its protein expression profile. Bile stress is one of the unfavorable conditions for inhibiting the growth of H. pylori in human body. Many studies have also shown that bile can inhibit the growth of H. pylori, but H. pylori can still exist in the duodenal bulb where bile is abundant, but infection of other hepatobiliary systems is rare. Therefore, we speculate that the acidification of bile affects the inhibitory activity of H. pylori. In order to study the adaptability of H. pylori to bile stress
【學位授予單位】：山東大學
【學位級別】：碩士
【學位授予年份】：2005
【分類號】：R378

【共引文獻】

相關期刊論文 前10條

1 梁麗;石川;;尿素(14)~C呼氣試驗與組織病理學和細菌培養(yǎng)在檢測幽門螺旋桿菌感染的比較[J];北方藥學;2012年05期

2 楊燕,趙海玲;~(13)C尿素呼氣試驗診斷小兒幽門螺桿菌感染[J];北京醫(yī)學;2001年02期

3 張小晉,程遠,王s，

本文編號：1541564