本文關鍵詞： 內皮型脾臟基質細胞 造血干細胞 分化 調節(jié)性樹突狀細胞 白細胞介素10 T細胞　出處：《浙江大學》2005年博士論文　論文類型：學位論文

【摘要】：樹突狀細胞(dendritic cells,DCs)是體內功能最強的抗原遞呈細胞。在過去的十年中,多數(shù)研究者的興趣是集中在DC是如何激發(fā)免疫應答的研究上,然而,近幾年的工作顯示DCs不但具有激發(fā)免疫反應的能力,而且,還可以下調免疫反應或者誘導免疫耐受的產(chǎn)生�，F(xiàn)在普遍認為,DC在激活na(?)ve T細胞和誘導耐受中都扮演關鍵的角色。DCs既可以誘導中樞耐受又可以誘導外周耐受,特別是DCs在誘導外周耐受中的作用越來越受到重視。最近,具有免疫負向調節(jié)功能的調節(jié)性DCs(regulatory dendritic cells)被陸續(xù)發(fā)現(xiàn),并成為這一研究領域的新熱點。 目前對調節(jié)性DCs的研究主要集中在對其起源和發(fā)育的研究上,所采用的方法多數(shù)是用免疫抑制性細胞因子誘導產(chǎn)生的,如在常規(guī)誘導DCs的培養(yǎng)基中加入IL-10或TGF-β。但這類方法過于簡化,不能反映在體的實際分化過程,因此產(chǎn)生的DCs的特性是否能代表一種天然的細胞群體并不十分明確。 造血細胞的體內發(fā)育過程是在一定的微環(huán)境的作用下進行的。所謂的微環(huán)境(microenvironment)或“龕位”(niche)是由成纖維細胞、巨噬細胞、內皮細胞、脂肪細胞等基質細胞和它們分泌的細胞外基質組成的,為造血細胞的發(fā)育提供各種必需的外源性信號的發(fā)育場所。不同的器官有不同的微環(huán)境,同一器官的不同區(qū)域的亞微環(huán)境也不同。不同的微環(huán)境可誘導產(chǎn)生不同的種類或功能特征的細胞。如骨髓的造血微環(huán)境。因此,脫離微環(huán)境來研究造血細胞的發(fā)育所得結論不可能反映機體的真實情況。 脾臟是機體最大的外周免疫器官,又是哺乳動物的造血器官,并且小鼠脾臟終身保持造血功能。經(jīng)典的脾結節(jié)形成實驗證明,脾臟的微環(huán)境不但具有支持造血的作用,而
[Abstract]:Dendritic cells ( DCs ) are the most potent antigen - presenting cells in vivo . In the past decade , the interest of most researchers has been focused on how DCs can stimulate immune responses . However , recent studies have shown that DCs play a key role in activating na ( ? ) ve T cells and inducing tolerance . It is generally believed that DCs play a key role in activating na ( ? ) ve T cells and inducing tolerance . DCs can both induce central tolerance and induce peripheral tolerance , especially DCs in inducing peripheral tolerance . Recently , regulatory DCs with immune negative regulatory functions have been recognized . cells ) have been found in succession and become a new focus in this field of research . At present , the study of regulatory DCs is mainly focused on the study of the origin and development of DCs , most of which are induced by immunosuppressive cytokines , such as the addition of IL - 10 or TGF - 尾 in the culture medium of conventional induced DCs , but this method is too simplified to reflect the actual differentiation of DCs , and thus whether the nature of DCs produced represents a natural population of cells is not very clear . In vivo development of hematopoietic cells is carried out under the action of a certain microenvironment . The so - called microenvironment or niche consists of stromal cells , such as fibroblasts , macrophages , endothelial cells , fat cells , and extracellular matrix , and provides various necessary exogenous signals for the development of hematopoietic cells . Different organs have different microenvironment , and the sub - microenvironment of different regions of the same organ is different . The spleen is the largest peripheral immune organ of the organism , and is the hematopoietic organ of the mammal , and the spleen of the mouse keeps the hematopoietic function for a lifetime . The classical spleen nodule formation experiment proves that the microenvironment of the spleen not only has the function of supporting the hematopoietic function , but also

【學位授予單位】：浙江大學
【學位級別】：博士
【學位授予年份】：2005
【分類號】：R392

【共引文獻】

相關博士學位論文 前2條

1 張明徽;脾基質細胞對造血前體細胞的定向誘導作用及對樹突狀細胞生物學特性的影響[D];第二軍醫(yī)大學;2002年

2 郭振紅;凋亡信號和免疫微環(huán)境對樹突狀細胞的免疫調控作用[D];第二軍醫(yī)大學;2005年

相關碩士學位論文 前1條

1 李志宏;脾臟抗原提呈細胞在多器官功能障礙綜合征免疫失衡中作用的研究[D];中國人民解放軍軍醫(yī)進修學院;2003年

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本文編號：1514778