本文關(guān)鍵詞： 腺苷A1受體 延髓腦片 面神經(jīng)后核內(nèi)側(cè)區(qū) 呼吸節(jié)律 雙相呼吸神經(jīng)元 吸氣神經(jīng)元　出處：《第一軍醫(yī)大學》2005年碩士論文　論文類型：學位論文

【摘要】：目的 1) 應用新生大鼠離體延髓腦片標本,記錄與之相連的舌下神經(jīng)呼吸節(jié)律性放電(respiratory rhythmical discharge activity,RRDA),觀察腺苷A1受體的特異性拮抗劑8-環(huán)戊-1,3-二丙基黃嘌呤(8-cyclopentyl-1,3-dipropylxa nthine,DPCPX)和特異性激動劑 R-苯異丙基-腺苷(R-phenylisoprpyl-adenosine,R-PIA)對RRDA的影響,從而探討腺苷A_1受體在節(jié)律性呼吸的產(chǎn)生和調(diào)節(jié)中的作用;2) 在新生大鼠離體延髓腦片標本上,同步記錄舌下神經(jīng)根和面神經(jīng)后核內(nèi)側(cè)區(qū)(the medial area of retrofacialis nucleus,mNRF)的雙相呼氣神經(jīng)元/吸氣神經(jīng)元的放電活動,觀察腺苷A1受體的特異性拮抗劑和激動劑對呼吸神經(jīng)元放電活動的影響,進一步探討腺苷A1受體對節(jié)律性呼吸的調(diào)制。 方法 選用新生SD大鼠(0～2d),雌雄不拘。1) 參照并改良Suzue的方法制作離體延髓腦片標本,主要包含面神經(jīng)后核內(nèi)側(cè)區(qū),前包欽格氏復合體(Pre-B(?)tzinger Complex)、腹側(cè)呼吸組以及背側(cè)呼吸組的一部分,保留舌下神經(jīng)根。用玻璃吸附電極記錄舌下神經(jīng)放電作為呼吸活動的指標,通過灌流液給藥,觀察不同藥物對神經(jīng)根放電活動的影響;2) 在面神經(jīng)后核內(nèi)側(cè)區(qū)(mNRF)同步記錄雙相呼氣神經(jīng)元/吸氣神經(jīng)元的放電活動,灌流給藥,觀察其對呼吸神經(jīng)元放電的影響。 結(jié)果 1.記錄舌下神經(jīng)根放電的實驗部分:1) DPCPX和R-PIA對RRDA的影響:與給藥前對照相比,應用2μmol/L DPCPX后,呼吸周期(RC)顯著性降低,呼氣時程(TE)顯著性縮短,RC縮短27.07%(P0.05),TE減少28.38%(P0.05),而吸氣時程(TI)和放電的積分幅度(IA)較對照無明顯變化,第15min的結(jié)果較第10min的變化不大。改用10μmol/L R-PIA灌流后15min
[Abstract]:Objective 1) to apply in vitro medulla oblongata of newborn rats. Respiratory rhythmic discharges associated with the hypoglossal nerve (rhythmical discharge activity) were recorded. To observe the specific antagonist of adenosine A1 receptor, 8-cyclopentyl-1-dipropylxa nthine. DPCPX and R-phenylisopr-adenosine R-PIA-a specific agonist on RRDA. Therefore, the role of adenosine A-1 receptor in the production and regulation of rhythmic respiration was discussed. 2) on the medulla oblongata of newborn rats. The medial area of retrofacialis nucleus were recorded simultaneously in the root of the hypoglossal nerve and the medial area of the posterior nucleus of the facial nerve. The effects of specific antagonists and agonists of adenosine A1 receptor on the activity of respiratory neurons were observed. To further investigate the modulation of adenosine A1 receptor on rhythmic respiration. Methods the medulla oblongata slices of neonatal SD rats were made by using the method of modified Suzue, including the medial area of the posterior nucleus of the facial nerve, according to the method of using male and female Sprague-Dawley rats for 2 days. Pre-Bu? The hypoglossal nerve root was preserved in the ventral breathing group and the dorsal breathing group. The hypoglossal nerve discharge was recorded with the glass adsorption electrode as the index of respiratory activity. The effects of different drugs on the activity of nerve root discharge were observed. 2) the discharges of biphasic expiratory / inspiratory neurons were recorded synchronously in the medial region of the posterior nucleus of the facial nerve, and the effects of MNRFs on the discharges of respiratory neurons were observed. Results 1. The effects of DPCPX and R-PIA on RRDA were recorded in the experimental part of hypoglossal nerve root discharges: compared with those before administration. After 2 渭 mol/L DPCPX, the respiratory cycle was significantly decreased, the expiratory duration was significantly shortened and RC was shortened by 27.07% (P 0.05). Te decreased 28.38% (P 0.05), but the inspiratory time interval (tie) and the integral amplitude of discharge (IAA) showed no significant change compared with the control group. The result of 15min was not different from that of 10min. 15 minutes after using 10 渭 mol/L R-PIA
【學位授予單位】：第一軍醫(yī)大學
【學位級別】：碩士
【學位授予年份】：2005
【分類號】：R338

【參考文獻】

相關(guān)期刊論文 前10條

1 張楓桐,吳中海,李有仁;阻滯面神經(jīng)后核內(nèi)側(cè)區(qū)對呼吸節(jié)律的影響[J];第一軍醫(yī)大學學報;1989年02期

2 吳中海,張楓桐,李有仁;損毀面神經(jīng)后核內(nèi)側(cè)區(qū)神經(jīng)元胞體對呼吸節(jié)律的影響[J];第一軍醫(yī)大學學報;1992年03期

3 吳中海,胡德輝,高楊,徐小元;新生大鼠離體延髓-脊髓標本的呼吸節(jié)律性放電及微切割延髓對其放電的影響[J];第一軍醫(yī)大學學報;1998年04期

4 高楊,胡德輝,吳中海;新生大鼠離體延髓面神經(jīng)后核內(nèi)側(cè)區(qū)微量注射GABA和BIC對呼吸節(jié)律的影響[J];第一軍醫(yī)大學學報;1999年03期

5 王寧黔,吳中海;大鼠面神經(jīng)后核內(nèi)側(cè)區(qū)與腦干呼吸相關(guān)區(qū)間纖維聯(lián)系[J];第一軍醫(yī)大學學報;2002年05期

6 吳中海，徐小元，張楓桐;面神經(jīng)后核內(nèi)側(cè)區(qū)微量注射GABA對節(jié)律性呼吸的影響[J];第一軍醫(yī)大學學報;1995年01期

7 吳中海,張楓桐,李有仁;家兔延髓區(qū)域阻滯對呼吸的影響[J];生理學報;1988年03期

8 吳中海,張楓桐,李有仁;家兔面神經(jīng)后核內(nèi)側(cè)區(qū)在呼吸節(jié)律起源中的作用[J];生理學報;1990年01期

9 吳中海,張楓桐,徐小元;延髓面神經(jīng)后核內(nèi)側(cè)區(qū)呼吸相關(guān)神經(jīng)元的放電形式[J];生理學報;1997年04期

10 胡德輝,吳中海,高揚;切割延髓、酸堿度及溫度對新生大鼠離體延髓——脊髓標本呼吸節(jié)律性放電的影響[J];中國應用生理學雜志;2001年01期

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