本文關鍵詞： 雜色曲霉素 室周器官 超微結構 TNF-α TGF-β2 凋亡　出處：《河北醫(yī)科大學》2006年博士論文　論文類型：學位論文

【摘要】： 目的:霉菌毒素對腦組織的損傷受到了國內外許多學者的廣泛關注,但是,雜色曲霉素(Sterigmatocystin, ST)對腦組織的影響國內外尚未見報道。室周器官(circumventricular organs, CVOs)是腦室周圍的一些微小器官,包括穹隆下器、正中隆起、終板血管器、脈絡叢、連合下器、松果體后葉及最后區(qū)等,由于其缺乏血腦屏障,因此是監(jiān)控血成分的理想位點。本實驗通過灌胃給藥構建ST動物實驗模型,研究給藥后不同時間點ST對CVOs生物學效應。觀察給藥后小鼠穹隆下器、脈絡叢和外側隱窩形態(tài)學變化及其室管膜和室管膜下細胞超微結構變化。檢測小鼠穹隆下器、脈絡叢和外側隱窩TNF-α、TGF-β2基因的表達情況。檢測小鼠穹隆下器、脈絡叢和外側隱窩和其他腦區(qū)細胞凋亡,探討ST對腦組織的影響及其機制。其意義在于首次研究ST毒素對CVOs的作用,為以后更加深入了解ST毒素對腦組織的影響奠定了一定的基礎,同時為研究其他毒素對腦組織的影響開辟了一條新的途徑。 方法: 1掃描電鏡和透射電鏡檢查 雄性BALB/c小鼠,體重18~20 g,由河北醫(yī)科大學實驗動物部提供。將18只BALB/c雄性小鼠隨機分為6組,每組3只。處理組5組,對照組1組,處理組按ST 3000μg/kg(sigma公司)的劑量,溶于0.5 ml生理鹽水中灌胃。對照組1組用等量的生理鹽水灌胃。灌胃后即刻處死動物,處理組動物于灌胃后1、2、4、8、16 h處死動物。 1.1掃描電鏡標本制備 麻醉動物后,開胸經心臟常規(guī)灌注,灌注液為1.5%多聚甲醛和2.5%戊二醛磷酸緩沖液(0.1 mol/L,pH7.2)。灌注完畢取出整腦,在固定劑中(4℃)后固定12 h。將修好的腦組織塊用磷酸緩沖液(0.1 mol/L,pH7.2)仔細沖洗3次,再放入磷酸緩沖液中過夜,梯度酒精脫水,叔丁醇置換,二氧化碳臨界點干燥,真空噴金,日立S-3500N掃描電鏡觀察、照像。
[Abstract]:Objective: the damage of mycotoxins to brain tissue has been widely concerned by many scholars at home and abroad, but Sterigmatocystin. The effects of ST) on brain tissue have not been reported at home and abroad. Periventricular organ circumventricular organs (CVOss) are some small organs around the ventricle. These include the subfornix, the median eminence, the endplate vascular apparatus, the choroid plexus, the subconjunctival apparatus, the posterior lobe of the pineal gland and the final area, due to its lack of blood-brain barrier. Therefore, it is an ideal site to monitor the blood composition. In this experiment, St animal model was established by intragastric administration, and the biological effects of St on CVOs were studied at different time points after administration, and the subfornial organ of mice was observed after administration. Morphological changes of choroid plexus and lateral recess and ultrastructural changes of ependymal and subependymal cells were observed. Expression of TGF- 尾 2 gene. Apoptosis of mouse subfornical organ, choroid plexus, lateral recess and other brain regions were detected. The purpose of this study is to study the effect of St toxin on CVOs for the first time, and to lay a foundation for further understanding the effect of St toxin on brain tissue. At the same time, it provides a new way to study the effects of other toxins on brain tissue. Methods: 1 scanning electron microscopy and transmission electron microscopy Male BALB/c mice, weighing 1820 g, were provided by the experimental animal department of Hebei Medical University. Eighteen BALB/c male mice were randomly divided into 6 groups, 3 in each group and 5 in treatment group. In the control group 1, the dose of ST3000 渭 g / kg sigma was used in the treatment group. The rats in the control group were given the same amount of normal saline. The animals in the treatment group were killed immediately after gastric perfusion, and the animals in the treatment group were killed at 816 hours after 1 hour of gastric perfusion. 1.1 preparation of SEM specimens After anesthesia, the chest was routinely perfused with 1.5% paraformaldehyde and 2.5% glutaraldehyde phosphate buffer (0.1 mol / L 路L ~ (2 +)). The whole brain was taken out after perfusion. After fixation at 4 鈩，

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