本文關(guān)鍵詞： 腎上腺素 堿基 電子轉(zhuǎn)移 氫鍵復(fù)合物 循環(huán)伏安 量子化學(xué)　出處：《曲阜師范大學(xué)》2007年碩士論文　論文類型：學(xué)位論文

【摘要】： 核酸DNA是生物的基本遺傳物質(zhì)，是遺傳信息的載體，基因表達(dá)的物質(zhì)基礎(chǔ)，對生物演化起著決定性作用，決定著生物的發(fā)展方向。而構(gòu)成核酸的基本單元主要是生物體內(nèi)存在著的堿基—胞嘧啶、胸腺嘧啶、尿嘧啶、腺嘌呤和鳥嘌呤，它們均為含有氮原子共軛體系的雜環(huán)化合物，對生物體系有重要作用。腎上腺素是哺乳動物和人類的一種重要的兒茶酚胺類神經(jīng)傳遞物質(zhì)，它控制著神經(jīng)系統(tǒng)進(jìn)行一系列生物反應(yīng)及神經(jīng)化學(xué)過程，腎上腺素也是一種重要的生物分子。研究生物分子間的相互作用是了解生物體系的特異作用和識別機(jī)理的基礎(chǔ)，研究堿基分子與腎上腺素的相互作用，對于推動生命科學(xué)、藥物化學(xué)、物理化學(xué)等學(xué)科的發(fā)展能起到一定的積極作用。本文用電化學(xué)方法研究了胞嘧啶、胸腺嘧啶、尿嘧啶三種堿基對腎上腺素電化學(xué)氧化的循環(huán)伏安(CV)曲線的影響，并輔助于量子化學(xué)方法對三種堿基與腎上腺素形成的氫鍵復(fù)合物進(jìn)行了理論探討。 利用循環(huán)伏安法研究了胞嘧啶、胸腺嘧啶、尿嘧啶在KRPB緩沖溶液中對腎上腺素電子轉(zhuǎn)移性質(zhì)的影響，實(shí)驗(yàn)結(jié)果表明：在KRPB緩沖溶液中，這幾種堿基對腎上腺素具有一定的穩(wěn)定作用，能在一定程度上抑制腎上腺素的氧化，從而改變了腎上腺素的循環(huán)伏安特征。由于胞嘧啶的溶解度在三者中較大，又研究了胞嘧啶在不同介質(zhì)中對腎上腺素氧化行為的影響，實(shí)驗(yàn)發(fā)現(xiàn)：在不同的體系中胞嘧啶對腎上腺素的穩(wěn)定作用不同，即體系組成物質(zhì)的不同對堿基與腎上腺素相互作用的強(qiáng)弱有一定的影響。 胞嘧啶、胸腺嘧啶、尿嘧啶在生物體系中主要以酮式結(jié)構(gòu)存在，，用量子化學(xué)方法優(yōu)化了它們的酮式結(jié)構(gòu)的幾何構(gòu)型，它們的酮式結(jié)構(gòu)能與腎上腺素形成氫鍵復(fù)合物，對它們形成的復(fù)合物進(jìn)行幾何構(gòu)型優(yōu)化，獲得了結(jié)構(gòu)參數(shù)和能量。同時對烯醇式結(jié)構(gòu)的胞嘧啶與質(zhì)子化的腎上腺素形成的氫鍵復(fù)合物進(jìn)行了幾何構(gòu)型優(yōu)化，獲得了相應(yīng)的結(jié)構(gòu)參數(shù)和能量，解釋了有關(guān)實(shí)驗(yàn)現(xiàn)象。
[Abstract]:Nucleic acid DNA is the basic genetic material of biology, is the carrier of genetic information, the material basis of gene expression, and plays a decisive role in biological evolution. The basic units of nucleic acid are the bases of cytosine, thymine, uracil, adenine and guanine. They are heterocyclic compounds containing conjugated nitrogen atoms and play an important role in biological systems. Epinephrine is an important catecholamines neurotransmitter in mammals and humans. It controls a series of biological reactions and neurochemical processes in the nervous system. Epinephrine is also an important biomolecules. Studying the interaction between biomolecules is the basis of understanding the specific action and recognition mechanism of biological systems and studying the interaction between base molecules and epinephrine. It can play a positive role in promoting the development of life science, pharmaceutical chemistry, physical chemistry and so on. In this paper, cytosine and thymine were studied by electrochemical method. The effect of three bases of uracil on the cyclic voltammetry (CV) curve of the electrochemical oxidation of epinephrine was studied. The hydrogen bond complexes formed between the three bases and epinephrine were investigated theoretically by quantum chemical method. The effects of cytosine, thymine and uracil on the electron transfer properties of epinephrine in KRPB buffer solution were studied by cyclic voltammetry. The experimental results showed that the effect of cytosine, thymine and uracil in KRPB buffer solution was studied. These bases have a certain stabilizing effect on epinephrine, which can inhibit the oxidation of epinephrine to a certain extent, thus changing the cyclic voltammetry of epinephrine. The effects of cytosine on epinephrine oxidation in different media were also studied. It was found that cytosine had different stabilizing effects on adrenaline in different systems. That is, the different composition of the system has a certain effect on the interaction between base and epinephrine. Cytosine, thymine and uracil mainly exist as ketones in biological systems. The geometric configurations of cytosine, thymidine and uracil are optimized by quantum chemical method. Their ketone structure can form hydrogen bond complexes with epinephrine, and the geometric configuration of the complexes is optimized. The structure parameters and energy were obtained, and the geometries of the hydrogen bond complexes formed by cytosine and protonated epinephrine were optimized, and the corresponding structural parameters and energy were obtained. The experimental phenomena are explained.
【學(xué)位授予單位】：曲阜師范大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2007
【分類號】：R341

【引證文獻(xiàn)】

相關(guān)碩士學(xué)位論文 前1條

1 王慧;腺嘌呤與腎上腺素相互作用的研究[D];曲阜師范大學(xué);2009年

本文編號：1457693