本文關(guān)鍵詞：從臍血單個(gè)核細(xì)胞誘導(dǎo)巨核細(xì)胞體外擴(kuò)增及分化的實(shí)驗(yàn)研究　出處：《福建醫(yī)科大學(xué)》2005年碩士論文　論文類(lèi)型：學(xué)位論文

  更多相關(guān)文章： 臍血 巨核細(xì)胞 細(xì)胞因子 體外擴(kuò)增 誘導(dǎo)分化

【摘要】：巨核細(xì)胞是血小板的前體細(xì)胞。將部分臍血進(jìn)行巨核系祖細(xì)胞體外擴(kuò)增后再輸注給患者,是有望解決臍血造血干細(xì)胞移植后患者血小板恢復(fù)遲緩這一難題的有效途徑,但目前最佳擴(kuò)增條件的確立仍處于實(shí)驗(yàn)階段。本項(xiàng)研究從臍血單個(gè)核(MNC)出發(fā),在無(wú)血清培養(yǎng)體系中比較了TPO、TPO+SCF、TPO+SCGF、TPO+IL-3、TPO+EPO5 種簡(jiǎn)單細(xì)胞因子組合對(duì)刺激巨核細(xì)胞擴(kuò)增的作用,經(jīng)體外培養(yǎng)14 天后,TPO+SCF 組CD41~+細(xì)胞比例和擴(kuò)增倍數(shù)最高,分別為20.95±2.83%和48.40±5.71倍;接著,將TPO 組和TPO+SCF 組的臍血MNC 均培養(yǎng)到18 天,以觀察培養(yǎng)后期(9d、14d、18d)巨核細(xì)胞的凋亡情況,結(jié)果發(fā)現(xiàn)TPO+SCF 組的MNC 和CD41~+細(xì)胞的凋亡率與單用TPO 組相比均有顯著差異(P0.05);最后,以TPO+SCF組為研究對(duì)象,采用RT-PCR 方法檢測(cè)了轉(zhuǎn)錄因子GATA-1、NF-E2 和系特異抗原GPⅡb mRNA 表達(dá)情況,發(fā)現(xiàn)培養(yǎng)14 天時(shí)三者的表達(dá)均達(dá)到高峰。上述結(jié)果證明:SCF 和TPO 的協(xié)同刺激在有效促進(jìn)巨核細(xì)胞的擴(kuò)增的同時(shí),也在有效誘導(dǎo)其分化。
[Abstract]:Megakaryocytes are progenitor cells of platelets. Some umbilical cord blood was amplified by megakaryocyte progenitor cells in vitro and then infused to patients. It is an effective way to solve the problem of delayed platelet recovery after cord blood hematopoietic stem cell transplantation. However, the optimal amplification conditions are still in the experimental stage. This study compared TPO SCF in serum-free culture system from cord blood mononuclear cells (MNCs). The effect of simple cytokine combination of TPO SCGF- TPO IL-3 TPO EPO5 on the proliferation of megakaryocytes cultured in vitro for 14 days. CD41 ~ ~ cells in TPO SCF group were 20.95 鹵2.83% and 48.40 鹵5.71 times respectively. Then, the cord blood MNC of TPO group and TPO SCF group were cultured for 18 days to observe the apoptosis of megakaryocyte in the late stage of culture. The results showed that the apoptosis rates of MNC and CD41 ~ ~ cells in TPO SCF group were significantly different from those in TPO group. Finally, the expression of transcription factor GATA-1NF-E2 and lineage-specific antigen GP 鈪，

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