本文關(guān)鍵詞：女性尿道括約肌的神經(jīng)分布：胎兒免疫組化研究　出處：《青島大學(xué)》2007年碩士論文　論文類型：學(xué)位論文

  更多相關(guān)文章： 控尿 神經(jīng)分布 尿道括約肌 免疫組織化學(xué)

【摘要】： 目的支配女性尿道括約肌神經(jīng)纖維精確的定性、定量和定位結(jié)論還沒有清楚地建立。以尸體解剖為基礎(chǔ)的有關(guān)陰部神經(jīng)和自主神經(jīng)纖維的大量解剖學(xué)研究為我們提供了許多矛盾的結(jié)論。本實(shí)驗設(shè)計在于通過對女性胎兒尿道的免疫組化分析，識別女性尿道括約肌的神經(jīng)分布。 方法選取12例21周至40周的正常女性胎兒尿道，分別進(jìn)行石蠟包埋、膽堿乙�；D(zhuǎn)移酶(choline acetylcatransferase，CHAT)及神經(jīng)肽Y(neuropeptide Y，NPY)免疫組織化學(xué)染色(SP法)，，并對其結(jié)果進(jìn)行半定量和定量統(tǒng)計學(xué)分析。 結(jié)果從尿道內(nèi)口至外口，神經(jīng)肽Y陽性區(qū)域顯色范圍逐漸變小、強(qiáng)度減弱，近1／3與遠(yuǎn)1／3，中1／3與遠(yuǎn)1／3之間差異均有極顯著性(P(0.01)，但近1／3與中1／3之間差異無顯著性(P＞0.05)。膽堿乙�；D(zhuǎn)移酶陽性區(qū)域主要位于尿道中1／3，近1／3次之，遠(yuǎn)1／3最弱，遠(yuǎn)、中、近端三者差異均具有極顯著性(P＜0.01)。 結(jié)論1．膽堿乙�；D(zhuǎn)移酶及神經(jīng)肽Y陽性區(qū)域主要位于女性尿道的中1／3，統(tǒng)計學(xué)上具有顯著性差異，是膽堿能及腎上腺素能神經(jīng)遞質(zhì)集中的部位；2．橫紋括約肌開始于尿道近1／3的遠(yuǎn)端，位于平滑肌的外側(cè)，向遠(yuǎn)端逐漸增厚，尿道中1／3為高峰，以后逐漸變�。�3．在女性胎兒尿道的中1／3處，平滑肌與橫紋括約肌之間沒有明顯的界限，可清楚地看到平滑肌和橫紋肌纖維互相交錯分布，逐漸過渡，形成所謂的尿道括約肌復(fù)合體。 尿道括約肌的膽堿乙�；D(zhuǎn)移酶及神經(jīng)肽Y免疫組織化學(xué)染色對理解參與控尿的尿道括約肌神經(jīng)支配提供了很有價值的幫助，對臨床實(shí)踐具有指導(dǎo)意義。
[Abstract]:Objective to control female urethral sphincter nerve fiber qualitative accurate, quantitative and positioning conclusion is not clearly established. A study on anatomy of the autopsy on the pudendal nerve and autonomic nerve fibers as the foundation provided conflicting results for us. The experimental design is through the immune group of female fetuses urethral nerve distribution analysis identification of female urethral sphincter.
The normal fetal female urethral methods 12 cases 21 to 40 weeks, respectively, were embedded in paraffin, choline acetyltransferase (choline acetylcatransferase, CHAT) and neuropeptide Y (neuropeptide Y NPY) immunohistochemical staining (SP method), and the results were analyzed quantitatively and semiquantitatively statistically.
The results from urethral mouth to mouth, neuropeptide Y positive area color range becomes smaller, the intensity of nearly 1 / 3 and 1 / 3 in the far, 1 / 3 and 1 / 3 far between the differences were very significant (P (0.01), but nearly 1 / 3 and 1 / 3 no significant difference (P > 0.05). B choline acyltransferase positive areas were mainly located in the urethra in 1 / 3, nearly 1 / 3, 1 / 3 is the weakest, far in the proximal three significantly (P < 0.01).
Conclusion 1. choline acetyltransferase and neuropeptide Y positive areas were mainly located in the female urethra in 1 / 3, a significant difference is the cholinergic and adrenergic neurotransmitter concentration areas; 2. in the distal urethral sphincter started nearly 1 / 3, is located outside the smooth muscle, gradually thickened distal to the in 1, urethral / 3 peak, then gradually thinning; 3. in the female fetus urethral / 1 3, there was no obvious difference between the smooth and striated sphincter, can clearly see the striated and smooth muscle fibres interveined each other, gradually, the formation of urethral sphincter complex matter.
Immunohistochemical staining of choline acetyltransferase and neuropeptide Y in urethral sphincter can provide valuable help for understanding the innervation of urethral sphincter, and is of guiding significance for clinical practice.

【學(xué)位授予單位】：青島大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2007
【分類號】：R323

【引證文獻(xiàn)】

相關(guān)博士學(xué)位論文 前1條

1 陳燕;膀胱尿道功能發(fā)育及尿失禁治療的臨床與基礎(chǔ)研究[D];鄭州大學(xué);2013年

本文編號：1426196