本文關(guān)鍵詞：日本血吸蟲多價DNA疫苗的研究　出處：《江蘇省血吸蟲病防治研究所》2005年碩士論文　論文類型：學(xué)位論文

  更多相關(guān)文章： 日本血吸蟲 多價DNA疫苗 磷酸丙糖異構(gòu)酶 NP30-(CDR3)6 免疫保護作用 免疫刺激序列

【摘要】：日本血吸蟲病至今仍是一種嚴重危害公眾健康的公共衛(wèi)生問題。我國每年要投入大量的人力、物力防治血吸蟲病,但由于血吸蟲保蟲宿主多,釘螺分布廣且難以消滅,吡喹酮化療為主的血防策略難以阻斷傳播,再感染頻繁發(fā)生,血吸蟲病的流行狀況仍沒有得到根本改善。研制安全、有效的血吸蟲病疫苗,控制家畜感染,減少傳播來源和對人體的威脅是WHO 提出的綜合防治血吸蟲病中的一個主要措施,且放在優(yōu)先發(fā)展的位置上。目前已有6 種曼氏血吸蟲候選疫苗分子得到WHO/TDR 認可。DNA 疫苗作為一種劃時代的新技術(shù),在抗血吸蟲感染領(lǐng)域里也取得了很多進展。本實驗室已構(gòu)建的日本血吸蟲磷酸丙糖異構(gòu)酶(TPI)DNA 疫苗,免疫小鼠獲得部分的保護作用,且在保蟲宿主豬研究中發(fā)現(xiàn)除具有抗感染作用外,還能減輕宿主肝臟蟲卵肉芽腫炎癥反應(yīng),肉芽腫平均面積明顯減小;管曉虹等對日本血吸蟲單克隆抗獨特型抗體NP30 的研究發(fā)現(xiàn),NP30 分子具有較好的抗感染免疫和抗病免疫功效,其H鏈CDR3區(qū)可能代表了腸相關(guān)抗原(GAA)的抗原分子模擬,起到相似的主動免疫作用,人工設(shè)計的日本血吸蟲單克隆抗獨特型抗體NP30 CDR3 區(qū)6 倍重復(fù)表位基因(CDR3)6的表達產(chǎn)物保留了抗體的親和性和特異性;因此TPI 和(CDR3)6 均為日本血吸蟲疫苗的候選分子,且兩者的作用形式和機理似各不相同,分別為酶性抗原分子與抗原模擬表位基因。本研究構(gòu)建含TPI 和(CDR3)6 這兩種抗原分子的多價DNA 疫苗,觀察其免疫保護效果,并初步探討其作用機理。鑒于本實驗室對于免疫刺激序列的研究發(fā)
[Abstract]:Schistosomiasis is still a serious public health problem in public health. China has to spend a lot of manpower and material resources of schistosomiasis prevention, but due to Schistosoma infection, snail widely distributed and difficult to destroy, schistosomiasis control strategy praziquantel to prevent the spread of infection, and frequent, the schistosomiasis epidemic situation is still have not been fundamentally improved. The development of safe and effective vaccine for schistosomiasis control, livestock infection, reduce the spread of source and threat to human is one of the main measures of comprehensive prevention and control of schistosomiasis proposed by WHO, and placed in the priority position. There are 6 kinds of vaccine candidate molecules of Schistosoma mansoni WHO/TDR recognized as a.DNA vaccine a new technology of epoch-making, much progress has also been made in the field of anti schistosome infection. The laboratory has constructed the Japanese blood fluke Triose phosphate isomerase (TPI) DNA vaccine immunized mice protection effect, and the infection of pigs found besides anti infection effect, but also reduce the host liver granuloma inflammatory reaction, the average area of granuloma obviously decreased; Study on Schistosoma japonicum monoclonal anti idiotypic antibody NP30 Guan Xiaohong found. The NP30 molecule has good anti infection immunity and disease resistance of immune function, the H chain CDR3 region may represent the gut associated antigen (GAA) to simulate antigen molecules, plays a role similar to active immunization, artificial Schistosoma japonicum monoclonal anti idiotypic antibody NP30 CDR3 region 6 times repeated epitope gene (CDR3) expression product 6 retained the affinity and specificity of antibodies; therefore TPI and (CDR3) of all 6 candidate molecules of Schistosoma japonicum vaccine, and their mechanism of action form and may vary, respectively. In order to mimic epitope genes for enzyme antigen molecules and antigens, we constructed a multivalent DNA vaccine containing two antigens of TPI and (CDR3) 6. We observed its protective effect and explored its mechanism.

【學(xué)位授予單位】：江蘇省血吸蟲病防治研究所
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2005
【分類號】：R392

【引證文獻】

相關(guān)碩士學(xué)位論文 前1條

1 魏敏;日本血吸蟲P14基因納米微球-DNA疫苗的初步研究[D];安徽醫(yī)科大學(xué);2011年

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本文編號：1426301