本文關(guān)鍵詞：窒息大鼠心肺復(fù)蘇模型的改良及復(fù)蘇后大鼠腦組織AQP4表達(dá)變化的研究　出處：《蘇州大學(xué)》2007年碩士論文　論文類型：學(xué)位論文

  更多相關(guān)文章： 窒息法 心肺復(fù)蘇模型 心臟停搏時(shí)間 腦含水量 心肺復(fù)蘇 腦組織含水量 水通道蛋白-4 亞低溫

【摘要】： 第一部分窒息大鼠心肺復(fù)蘇模型的改良 目的:改良窒息大鼠心肺復(fù)蘇模型;探討心臟停搏持續(xù)時(shí)間對(duì)自主循環(huán)恢復(fù)后6h存活率和腦含水量的影響,為后續(xù)實(shí)驗(yàn)確定合適的心臟停搏時(shí)間。 方法:健康雄性SD大鼠36只,隨機(jī)分為4組:對(duì)照組(n=6),CA1min組、CA3min組、CA5min組,后三組每組均為10只。對(duì)照組僅予氣管切開(kāi)插管、股動(dòng)、靜脈置管,手術(shù)后6h處死。其它各組予氣管切開(kāi)插管、股動(dòng)、靜脈置管,呼氣末夾閉氣管導(dǎo)管模擬窒息,心臟停搏(cardiac arrest , CA)持續(xù)1min、3min、5min后實(shí)施心肺復(fù)蘇,應(yīng)用心電監(jiān)護(hù)儀監(jiān)測(cè)三組大鼠心肺復(fù)蘇期間平均動(dòng)脈壓(mean arterial pressure,MAP)、心率(heart rate,HR)及體溫的動(dòng)態(tài)變化,股動(dòng)脈采血監(jiān)測(cè)血?dú)獾膭?dòng)態(tài)變化,并觀察三組大鼠自主循環(huán)恢復(fù)(restoration of spontaneous circulation,ROSC)時(shí)間、復(fù)蘇成功率及ROSC后6小時(shí)的存活率及腦含水量(brain water content,BWC)。 結(jié)果:CA3min組的復(fù)蘇成功率、ROSC后6h存活率與CA5min組比較,有顯著性差異。CA3min組BWC與CA1min比較有顯著性差異(P㩳0.05)。 結(jié)論:1.改進(jìn)窒息大鼠心肺復(fù)蘇模型,改良后的模型更加穩(wěn)定,復(fù)蘇成功率和ROSC后6h存活率均顯著高于未改良前。 2.心臟停搏3min后復(fù)蘇成功率和ROSC后6h存活率均較高,ROSC后6h的BWC增加明顯。利用窒息大鼠進(jìn)行心肺復(fù)蘇后腦水腫研究,可選擇3min為實(shí)驗(yàn)心臟停搏時(shí)間。 第二部分心肺復(fù)蘇后大鼠腦組織水通道蛋白-4及其mRNA的動(dòng)態(tài)變化和亞低溫對(duì)其影響的研究 目的:探討復(fù)蘇后早期腦水腫的變化規(guī)律,AQP4及其mRNA在腦水腫發(fā)生、發(fā)展中的作用,以及亞低溫減輕心肺復(fù)蘇后腦水腫的可能機(jī)制。 方法:雄性SD大鼠66只,隨機(jī)分為3組,分別為對(duì)照組、常溫復(fù)蘇組、亞低溫組,后兩組各自進(jìn)一步分為5個(gè)亞組,即自主循環(huán)恢復(fù)后0.5、1、3、6和9小時(shí)組。建立窒息大鼠心肺復(fù)蘇模型,應(yīng)用干濕重法測(cè)定自主循環(huán)恢復(fù)后0.5、1、3、6和9小時(shí)腦組織含水量的變化;同時(shí)觀察各時(shí)間點(diǎn)腦組織形態(tài)學(xué)的變化。利用免疫組織化學(xué)染色半定量分析的方法,檢測(cè)相同觀察時(shí)點(diǎn)腦組織AQP4表達(dá)水平的變化。運(yùn)用RT-PCR方法檢測(cè)腦組織AQP4mRNA表達(dá)的變化,分析AQP4、AQP4mRNA與BWC的相關(guān)性。 結(jié)果:1.常溫組大鼠,ROSC后0.5hBWC開(kāi)始增加,隨著時(shí)間的延長(zhǎng)BWC明顯增加,各觀察時(shí)點(diǎn)BWC與對(duì)照組比較,均有顯著性差異(P值0.05)。腦組織形態(tài)學(xué)的變化與以上結(jié)果相符。同時(shí),復(fù)蘇后腦組織AQP4蛋白水平、AQP4mRNA的表達(dá),也呈現(xiàn)出與BWC變化幾乎相同的趨勢(shì)。相關(guān)分析表明,BWC與腦組織AQP4、AQP4mRNA水平表達(dá)呈明顯正相關(guān),相關(guān)系數(shù)(r)分別為0.847和0.771,(P0.01)。 2. ROSC后0.5小時(shí),亞低溫組大鼠BWC和腦組織AQP4表達(dá)水平與常溫組同一觀察時(shí)點(diǎn)比較無(wú)明顯變化;亞低溫作用1h、3h、6h和9h時(shí),上述指標(biāo)均較相同觀察時(shí)點(diǎn)常溫復(fù)蘇組明顯降低(P值均0.05)。相關(guān)分析表明,亞低溫作用9h內(nèi),BWC與腦組織AQP4、AQP4mRNA表達(dá)水平呈明顯正相關(guān), r分別為0.769和0.780,( P0.01)。 結(jié)論:1.心肺復(fù)蘇后0.5h已出現(xiàn)腦水腫,并隨著ROSC時(shí)間點(diǎn)的延長(zhǎng),腦水腫逐漸加重。 2.腦組織AQP4及其mRNA在ROSC后0.5h表達(dá)上調(diào),并隨著ROSC時(shí)間點(diǎn)的延長(zhǎng),逐漸增高,腦水腫嚴(yán)重程度和AQP4、AQP4mRNA均呈現(xiàn)明顯正相關(guān)。提示AQP4mRNA可能通過(guò)調(diào)節(jié)AQP4表達(dá),參與了心肺復(fù)蘇后腦水腫的過(guò)程。 3. ROSC后亞低溫干預(yù)可減輕腦水腫,其機(jī)制可能和亞低溫抑制AQP4mRNA,下調(diào)AQP4的表達(dá)有關(guān)。
[Abstract]:The improvement of the model of cardiopulmonary resuscitation in the first part of asphyxiated rats
Objective: to improve the cardiopulmonary resuscitation model of asphyxia rats, and to explore the effect of cardiac arrest duration on 6h survival rate and cerebral water content after the recovery of autonomic circulation, so as to establish suitable cardiac arrest time for subsequent experiments.
Methods: 36 healthy male SD rats were randomly divided into 4 groups: control group (n=6), CA1min group, CA3min group, CA5min group, three groups with 10 rats in each group. The control group only received tracheotomy, femoral artery, venous catheter, surgery after 6h death. Other groups were given trachea incision intubation, femoral artery, venous catheter, end expiratory clamping the endotracheal tube to simulate suffocation, cardiac arrest (cardiac arrest, CA) for 1min, 3min, 5min after CPR, cardiopulmonary resuscitation during the application of ECG monitoring in three groups of rats mean arterial pressure (mean arterial pressure, MAP), heart rate (heart rate, HR) and the dynamic changes of body temperature, the dynamic changes of femoral artery blood gas monitoring, and to observe the three groups of rats (restoration of spontaneous ROSC circulation, ROSC), the survival rate of water and brain 6 hours with the success rate of resuscitation and ROSC (brain water content, BWC).
Results: the successful rate of resuscitation in the CA3min group and the survival rate of 6h after ROSC were compared with that of the CA5min group, and there was a significant difference between BWC and CA1min in.CA3min group (P? 0.05).
Conclusion: 1. the model of cardiopulmonary resuscitation in asphyxiated rats was improved. The modified model was more stable, the success rate of resuscitation and the survival rate of 6h after ROSC were significantly higher than that before the improvement.
2., after cardiac arrest, the success rate of 3min recovery and the survival rate of 6h after ROSC were all higher. The BWC of 6h increased significantly after ROSC. The study of cerebral edema after cardiopulmonary resuscitation can be done by asphyxia rats. 3min can be selected as experimental cardiac arrest time.
The dynamic changes of aquaporin -4 and its mRNA in the brain tissue of rats after cardiopulmonary resuscitation and the effect of mild hypothermia on it in second parts
Objective: To investigate the change rule of brain edema in early stage after resuscitation, the role of AQP4 and mRNA in the development and development of cerebral edema, and the possible mechanism of mild hypothermia to relieve brain edema after cardiopulmonary resuscitation.
Methods: 66 male SD rats were randomly divided into 3 groups, including control group, normothermia group and hypothermia group, the two groups after each further divided into 5 subgroups, namely after the restoration of spontaneous circulation 0.5,1,3,6 and 9 hour group. To establish a rat model of cardiopulmonary resuscitation of asphyxia, changes of spontaneous circulation after 9 hours of recovery of 0.5,1,3,6 and brain water content measured by dry wet weight method; at the same time, the morphological changes of brain tissue were observed at different time points. Using immunohistochemistry and semi quantitative analysis, detection of the same observation point in brain tissue AQP4 expression. Using RT-PCR method to detect the expression of changes in brain tissue AQP4mRNA AQP4 analysis and the correlation between AQP4mRNA and BWC.
Results: 1. normal rats, ROSC 0.5hBWC began to increase, with the extension of time BWC increased significantly, compared with the control at each observation time point of BWC, there were significant differences (P = 0.05). The morphological changes of brain tissue coincided with the results above. At the same time, the recovery of brain tissue AQP4 protein level, the expression of AQP4mRNA also, showing almost the same trend with the change of BWC. Correlation analysis showed that BWC and AQP4 in brain tissue, the level of AQP4mRNA expression was positively correlated, correlation coefficient (R) were 0.847 and 0.771 (P0.01).
0.5 hours after 2. ROSC, hypothermia group BWC and rat brain tissue AQP4 expression level and the normal group at the same observation time there is no obvious change; mild hypothermia effects of 1H, 3h, 6h and 9h, the above indexes were observed in the same normothermia group significantly decreased (P < 0.05). Correlation analysis showed that analysis of the effect of the mild hypothermia in 9h, BWC and AQP4 in brain tissue, the expression level of AQP4mRNA was positively correlated with R, respectively 0.769 and 0.780 (P0.01).
Conclusion: 1. 0.5h has cerebral edema after cardiopulmonary resuscitation, and the brain edema gradually increases with the prolongation of ROSC time.
2., the expression of AQP4 and mRNA in brain tissue increased after ROSC, and increased with the extension of ROSC time. The severity of brain edema was positively correlated with AQP4 and AQP4mRNA. It suggested that AQP4mRNA might participate in the process of cerebral edema after regulating the expression of AQP4 in cardiopulmonary resuscitation.
Hypothermia intervention after 3. ROSC can reduce brain edema, and its mechanism may be related to subhypothermia inhibition of AQP4mRNA and down regulation of the expression of AQP4.

【學(xué)位授予單位】：蘇州大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2007
【分類號(hào)】：R-332

【參考文獻(xiàn)】

相關(guān)期刊論文 前8條

1 尹衛(wèi)東,郝貫一,宋來(lái)君,楊波;亞低溫治療大鼠腦外傷時(shí)腦溫測(cè)量技術(shù)[J];河南醫(yī)科大學(xué)學(xué)報(bào);1999年03期

2 陳壽權(quán),李章平,王姍姍,黃唯佳,程俊彥,王萬(wàn)鐵,王為;窒息法致大鼠心臟驟停模型復(fù)蘇的影響因素[J];中華急診醫(yī)學(xué)雜志;2005年10期

3 魯宏,孫善全;水通道蛋白-4在早期缺血性腦水腫中的表達(dá)[J];解剖學(xué)雜志;2003年04期

4 王姍姍;陳壽權(quán);李章平;王衛(wèi);王萬(wàn)鐵;;大鼠心肺復(fù)蘇后腦水腫的變化及腎上腺素對(duì)腦水腫的影響[J];實(shí)用醫(yī)學(xué)雜志;2006年10期

5 閻蕾;董瑞國(guó);曾因明;耿德勤;;亞低溫對(duì)大鼠缺血性腦水腫及水通道蛋白AQP4表達(dá)的影響(英文)[J];中國(guó)臨床康復(fù);2006年38期

6 代大偉;王德生;李克深;毛穎;張黎明;段淑榮;盛麗;;局部亞低溫對(duì)大鼠腦出血后水通道蛋白4表達(dá)的影響[J];中華醫(yī)學(xué)雜志;2006年13期

7 李燕華,孫善全;大鼠腦出血后AQP4mRNA的表達(dá)與Ca~(2+)關(guān)系的探討[J];中國(guó)急救醫(yī)學(xué);2003年07期

8 任麗,孫善全;AQP_4基因表達(dá)與缺血再灌注后腦水腫的關(guān)系[J];中國(guó)急救醫(yī)學(xué);2003年11期

，

本文編號(hào)：1408326