本文關(guān)鍵詞：Id2在成年大鼠腦中的表達(dá)及其影響SVZa神經(jīng)干細(xì)胞發(fā)育分化的初步研究　出處：《第三軍醫(yī)大學(xué)》2005年碩士論文　論文類型：學(xué)位論文

  更多相關(guān)文章： Id2 大鼠 腦 SVZa 神經(jīng)干細(xì)胞 RMS 發(fā)育 分化

【摘要】：隨著對(duì)神經(jīng)干細(xì)胞較為深入的研究,位于側(cè)腦室附近的室管膜前下區(qū)(Anterior subvcntricular zone,SVZa)和海馬被公認(rèn)為成年哺乳動(dòng)物神經(jīng)系統(tǒng)中神經(jīng)干細(xì)胞最為集中的部位。研究發(fā)現(xiàn),SVZa區(qū)神經(jīng)干細(xì)胞產(chǎn)生后,沿喙側(cè)遷移流(Rostral migratory stream,RMS)朝嗅腦方向遷移,成為出生后嗅腦(球)(Olfactory bulb,OB)神經(jīng)元終身更新的來源。SVZa區(qū)神經(jīng)干細(xì)胞除具有一般神經(jīng)干細(xì)胞特征外,還有其自身特點(diǎn),即在向OB遷移的過程中(RMS區(qū))始終保持不分化狀態(tài),在到達(dá)嗅腦后再分化為成熟神經(jīng)細(xì)胞和神經(jīng)膠質(zhì)細(xì)胞。 神經(jīng)干細(xì)胞分化是外源性信號(hào)與內(nèi)源性信號(hào)共同作用的結(jié)果,外源性信號(hào)如有絲分裂因子EGF、T_3、維甲酸、糖皮質(zhì)激素、血清等,內(nèi)源性信號(hào)如T_3核受體等都對(duì)神經(jīng)干細(xì)胞分化具有調(diào)控作用。我們研究組在以前的研究中發(fā)現(xiàn)細(xì)胞因子BMPs、Mashl、Pax6、Wnt等對(duì)SVZa區(qū)神經(jīng)干細(xì)胞的增殖、遷移、分化具有一定調(diào)控作用,這些因子在RMS區(qū)表達(dá)均很弱,且都有促進(jìn)神經(jīng)干細(xì)胞分化的作用。但SVZa區(qū)神經(jīng)干細(xì)胞在向OB遷移的RMS區(qū)始終保持不分化狀態(tài)的調(diào)控機(jī)制是什么呢?目前的研究還不清楚。神經(jīng)干細(xì)胞分化是正、負(fù)雙向調(diào)控因子共同作用的結(jié)果,我們課題組已經(jīng)研究了部分正向調(diào)控因子的作用,但抑制SVZa區(qū)神經(jīng)干細(xì)胞在遷移過程中分化的機(jī)制是什么呢?由于Id2具有其負(fù)向調(diào)控因子的特點(diǎn)(見后),因此我們選擇了Id2作為研究對(duì)象,期望為進(jìn)一步闡明SVZa區(qū)神經(jīng)干細(xì)胞在遷移過程中保持不分化的調(diào)控機(jī)制積累更豐富的資料。 含HLH結(jié)構(gòu)域的蛋白可分為兩類:堿性螺旋-環(huán)-螺旋(basic helix-loop-helix,bHLH)和Id(inhibitor of DNA binding)。bHLH可分為A型和B型。A型bHLH為一種遍在蛋白,它既可以與正調(diào)控蛋白聚合,又可以與負(fù)調(diào)控蛋白聚合;B型bHLH蛋白為正調(diào)控蛋白。Id則為負(fù)調(diào)控蛋白,是bHLH因子的抑制因子,其家族有Id1、Id2、Id3、Id4共4個(gè)成員。與bHLH蛋白不同,Id蛋白缺乏結(jié)合DNA的堿性區(qū)域。B型bHLH蛋白能與A型bHLH蛋白形成同源或異源二聚體,此二聚體再與E-box蛋白結(jié)合來實(shí)現(xiàn)其對(duì)下游基因的轉(zhuǎn)錄。Id與A型bHLH蛋白結(jié)合形成二聚體,從而干擾B型bHLH蛋白與A型bHLH蛋白結(jié)合形成同源或異源二聚體。最初的研究發(fā)現(xiàn),Id蛋白一般在
[Abstract]:With the further study of neural stem cells, the anterior and inferior ependymal subvcntricular zone is located near the lateral ventricle. SVZA) and hippocampus are recognized as the most concentrated sites of neural stem cells in adult mammalian nervous system. Rostral migratory streamer moved towards olfactory brain and became olfactory bulb after birth. The source of lifelong renewal of OB neurons. SVZa neural stem cells have their own characteristics in addition to the general characteristics of neural stem cells. In the process of migration to OB, the RMS region remained undifferentiated, and was then redifferentiated into mature nerve cells and glial cells after reaching the olfactory brain. Neural stem cell differentiation is the result of both exogenous and endogenous signals. Exogenous signals such as EGFT _ 3, retinoic acid, glucocorticoid, serum, and so on. Endogenous signals, such as T _ S _ 3 nuclear receptors, regulate the differentiation of neural stem cells. Our team found the cytokine BMPsMashln Pax6 in previous studies. Wnt and so on have certain regulation on the proliferation, migration and differentiation of neural stem cells in SVZa region, and these factors are very weak in the RMS region. But what is the regulatory mechanism of the neural stem cells in the SVZa region to remain undifferentiated in the RMS region that migrate to OB? The differentiation of neural stem cells is the result of both positive and negative bidirectional regulatory factors. Our team has studied the role of some positive regulatory factors. But what is the mechanism that inhibits the differentiation of neural stem cells in the SVZa region during migration? Because Id2 has the characteristics of negative regulatory factors (see back), we chose Id2 as our research object. It is expected to further clarify the regulatory mechanism of neural stem cells (NSCs) in the SVZa region to maintain undifferentiation during migration. Proteins containing HLH domain can be classified into two categories: basic helix-loop-helix. BHLH) and Id(inhibitor of DNA binding).bHLH can be divided into A type and B type. A type bHLH is a kind of protein. It can be polymerized with both positive and negative regulatory proteins. Type B bHLH protein is a positive regulatory protein. ID is a negative regulatory protein. It is an inhibitory factor of bHLH factor, and its family has Id1, Id2, Id3. There are 4 members of Id4. Unlike bHLH protein, the basic region. B type bHLH protein, which lacks binding to DNA, can form homologous or heterodimer with type A bHLH protein. The dimer then binds to E-box protein to achieve the transcription of downstream gene. ID binds to type A bHLH protein to form a dimer. It interferes with the binding of type B bHLH protein to type A bHLH protein to form homologous or heterodimer.
【學(xué)位授予單位】：第三軍醫(yī)大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2005
【分類號(hào)】：R329

【共引文獻(xiàn)】

相關(guān)期刊論文 前10條

1 王嵩;張麗霞;柴勇;楊成;;培養(yǎng)胚胎神經(jīng)干細(xì)胞向少突膠質(zhì)細(xì)胞誘導(dǎo)分化的實(shí)驗(yàn)研究[J];濱州醫(yī)學(xué)院學(xué)報(bào);2011年01期

2 陳興書,姚忠祥,劉建軍,陳建芳,楊輝,蔡文琴;Id2在成年大鼠喙側(cè)神經(jīng)干細(xì)胞遷移流通道的表達(dá)[J];第三軍醫(yī)大學(xué)學(xué)報(bào);2005年10期

3 張治元;楊輝;劉仕勇;何家全;邱克軍;宋業(yè)純;;Id2在SVZa神經(jīng)干細(xì)胞向神經(jīng)元分化中的作用[J];第三軍醫(yī)大學(xué)學(xué)報(bào);2006年10期

4 羅雪;陳興書;蔡其燕;鐘善傳;姚忠祥;;Id2在三碘甲狀腺氨酸調(diào)節(jié)大鼠室管膜前下區(qū)神經(jīng)干細(xì)胞分化中的作用[J];第三軍醫(yī)大學(xué)學(xué)報(bào);2009年05期

5 呂威力;邢雪松;張玲;張岷;姜海波;;bFGF在局灶性腦缺血后內(nèi)源性神經(jīng)干細(xì)胞增殖過程中對(duì)Id2的影響[J];解剖學(xué)研究;2011年01期

6 吳波;任先軍;郭樹章;;少突膠質(zhì)前體細(xì)胞移植治療脊髓損傷[J];脊柱外科雜志;2007年06期

7 劉玉波;于小玲;趙輝;;前列腺癌組織BMP2表達(dá)及其與DIF-1的相關(guān)性[J];齊魯醫(yī)學(xué)雜志;2011年02期

8 張敬華;朱建幸;;甲狀腺激素對(duì)少突膠質(zhì)細(xì)胞分化調(diào)控研究進(jìn)展[J];中國(guó)新生兒科雜志;2010年04期

9 張健;楊揚(yáng);朱樹干;遲令懿;李峰;王旭平;劉春喜;;胰島素樣生長(zhǎng)因子-1誘導(dǎo)神經(jīng)干細(xì)胞向少突膠質(zhì)細(xì)胞分化研究[J];中華實(shí)驗(yàn)外科雜志;2006年12期

10 楊慧敏;王順和;;神經(jīng)干細(xì)胞定向分化為少突膠質(zhì)細(xì)胞的實(shí)驗(yàn)研究[J];重慶醫(yī)科大學(xué)學(xué)報(bào);2007年08期

，

本文編號(hào)：1373871