本文關(guān)鍵詞：膜分子RCAS1在誘導(dǎo)免疫細(xì)胞反應(yīng)中的作用及機(jī)制研究　出處：《浙江大學(xué)》2006年碩士論文　論文類型：學(xué)位論文

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【摘要】：1996年,Sonoda(Cancer,1996)等建立了一種子宮腺癌細(xì)胞系SiSo,他們將SiSo免疫小鼠,利用雜交瘤技術(shù)制備了一系列單克隆抗體,發(fā)現(xiàn)了一種可被22-1-1單抗識別的新蛋白,分子量為40kD和78kD(認(rèn)為78KD蛋白為40KD蛋白的二聚體),命名為22—1—1抗原,該抗原不同于以往發(fā)現(xiàn)的YH206,GA733,CA125,CEA等腫瘤抗原,認(rèn)為是一種新的腫瘤相關(guān)抗原。Watanabe(Mol Cell Biol,1998)等在1998年從人乳腺癌細(xì)胞系MCF-7 cDNA文庫中克隆到一個(gè)雌激素反應(yīng)性的新基因,命名為EBAG9(Estrogen receptor-binding fragment-associated gene 9),編碼區(qū)為639bp,在5'上游有一個(gè)雌激素反應(yīng)元件,該分子mRNA和蛋白的表達(dá)隨雌激素的誘導(dǎo)而上調(diào)。Nakashima(Nat Med,1999)等分離鑒定出編碼22—1—1抗原的基因命名為RCAS1(receptor-binding cancer antigen expressed on SiSo cells),基因序列比對后發(fā)現(xiàn)RCAS1同EBAG9實(shí)際上是同一種蛋白質(zhì)。 關(guān)于RCAS1分子的大多數(shù)研究是用22-1-1單抗通過免疫組化實(shí)驗(yàn)分析在各種臨床腫瘤組織標(biāo)本中(如宮頸癌、侵襲性宮頸鱗狀上皮癌、乳腺癌、T3胃癌等)的表達(dá),認(rèn)為RCAS1的表達(dá)與腫瘤的分化、侵襲性和惡性程度呈正相關(guān),并提示不良預(yù)后,RCAS1在正常組織中沒有表達(dá)。RCAS1可作為腫瘤惡性程度
[Abstract]:In 1996, Sonoda (Cancer, 1996) a uterine adenocarcinoma cell line SiSo, they will SiSo immunized mice were prepared by hybridoma technique for a series of monoclonal antibodies, found a new protein can be identified by mAb 22-1-1, molecular weight of 40kD and 78kD (think 78KD 40KD protein two), dimer named 22 - 1 - 1 antigen, the antigen is different from previously found in YH206, GA733, CA125, CEA tumor antigen, that is a new tumor associated antigen.Watanabe (Mol Cell Biol, 1998) to clone a new gene estrogen responsive in 1998 from human breast cancer cell line MCF-7 cDNA library, named EBAG9 (Estrogen receptor-binding fragment-associated gene 9), encoding region of 639bp, an estrogen response element in the upstream of 5', induce the expression of the mRNA molecule and protein with estrogen and upregulation of.Nakashima (Nat Med, 199 9) isolation and identification of genes encoding 22 - 1 - 1 antigens is named RCAS1 (receptor-binding cancer antigen expressed on SiSo cells). After sequence alignment, RCAS1 is actually the same protein as EBAG9.
Most research on the RCAS1 molecule is 22-1-1 monoclonal antibody by immunohistochemistry experiments in a variety of clinical tumor samples (such as cervical cancer, invasion of cervical squamous cell carcinoma, breast cancer, gastric cancer T3 expression, etc.) that RCAS1 expression and tumor differentiation, invasion and malignant degree of positive correlation, and prompt poor prognosis, there was no expression of RCAS1.RCAS1 can be used as the degree of malignancy in normal tissues

【學(xué)位授予單位】：浙江大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2006
【分類號】：R392

【參考文獻(xiàn)】

相關(guān)期刊論文 前1條

1 朱學(xué)軍,劉志紅,劉棟,陳朝紅,黎磊石;雷公藤內(nèi)酯醇對人樹突狀細(xì)胞體外發(fā)育及免疫學(xué)功能的影響[J];腎臟病與透析腎移植雜志;2001年03期

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