本文關(guān)鍵詞：心理應(yīng)激對(duì)小鼠行為和免疫功能的影響　出處：《山西醫(yī)科大學(xué)》2006年碩士論文　論文類型：學(xué)位論文

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【摘要】： 目的:通過觀察慢性心理應(yīng)激對(duì)小鼠情緒行為和認(rèn)知能力的影響以及免疫器官的變化、血清中IFN-γ和IL-4水平和Th1和Th2細(xì)胞的失衡狀況,探討慢性心理應(yīng)激對(duì)心理行為和免疫功能的影響及可能的機(jī)制,為亞健康人群開展有效的防護(hù)和干預(yù)提供理論依據(jù)。 方法: BALB/c小鼠40只,隨機(jī)分為對(duì)照組、5天應(yīng)激組、10天應(yīng)激組、15天束縛應(yīng)激組,每組10只。采用自制的束縛筒對(duì)小鼠進(jìn)行連續(xù)束縛的方法建立慢性心理應(yīng)激模型,應(yīng)激結(jié)束后采用曠場(chǎng)實(shí)驗(yàn)和Morris水迷宮實(shí)驗(yàn)觀察小鼠應(yīng)激狀態(tài)下的行為變化;處死,測(cè)量各組小鼠胸腺、脾臟指數(shù)及脾和腸系膜淋巴結(jié)淋巴細(xì)胞數(shù)量,并以ELISA法檢測(cè)其血清IFN-γ和IL-4的水平及IL-4/IFN-γ比值的變化。 結(jié)果:心理應(yīng)激使小鼠在曠場(chǎng)實(shí)驗(yàn)中的潛伏期顯著延長(zhǎng)(P0.01),探究活動(dòng)減少(P0.05和P0.01),糞便次數(shù)增多(P0.05和P0.01),Morris水迷宮中各應(yīng)激組小鼠尋找平臺(tái)的潛伏期較對(duì)照組延長(zhǎng)(P0.05和P0.01),在空間探索實(shí)驗(yàn)中,應(yīng)激組小鼠在平臺(tái)所在象限的游泳時(shí)間比例均小于對(duì)照組。提示慢性心理應(yīng)激可導(dǎo)致小鼠焦慮、抑郁和認(rèn)知功能的障礙。應(yīng)激組小鼠的體重始終受到抑制低于對(duì)照組,差異顯著(P0.05和P0.01)。各應(yīng)激組小鼠的脾臟指數(shù)及脾和腸系膜淋巴結(jié)淋巴細(xì)胞數(shù)量受到明顯抑制,5天應(yīng)激組脾臟指數(shù)明顯降低(P0.05),10和15天應(yīng)激組顯著低于對(duì)照組(P0.01),而胸腺指數(shù)雖低于對(duì)照但無顯著意義。IFN-γ、IL-4/IFN-γ比值明顯下降,與正常對(duì)照比較(P0.05和P0.01),而IL-4水平無顯著性差異,提示心理應(yīng)激抑制小鼠的體重和免疫功能,導(dǎo)致Th1/Th2平衡失調(diào),向Th2細(xì)胞漂移,并且隨著應(yīng)激時(shí)間的延長(zhǎng)變化越明顯。脾臟指數(shù)及脾和腸系膜淋巴結(jié)淋巴細(xì)胞、IFN-γ與穿格、修飾之間呈顯著正相關(guān),與中央格停留時(shí)間和糞便呈負(fù)相關(guān)。提示應(yīng)激所致的情緒行為和認(rèn)知障礙可能與免疫功能的抑制有關(guān)。 結(jié)論:慢性心理應(yīng)激可以引起機(jī)體的情緒行為和認(rèn)知功能的改變,主要表現(xiàn)為情緒的焦慮、抑郁和學(xué)習(xí)記憶、適應(yīng)、反應(yīng)等認(rèn)知能力的下降;慢性心理應(yīng)激可以影響體重和免疫功能,導(dǎo)致機(jī)體的體重的改變和免疫力的下降,從而引起體質(zhì)衰弱、病感或疾病;心理應(yīng)激引起情緒行為和認(rèn)知功能的改變與免疫功能相關(guān)。因此,心理應(yīng)激引起機(jī)體的心理行為和免疫功能的改變可能是亞健康狀態(tài)的主要原因。
[Abstract]:Objective: to observe the effects of chronic psychological stress on emotional behavior and cognitive ability, the changes of immune organs, the levels of IFN- 緯 and IL-4 in serum and the imbalance of Th1 and Th2 cells in mice. To explore the influence of chronic psychological stress on psychological behavior and immune function and its possible mechanism, to provide theoretical basis for effective protection and intervention of sub-healthy population. Methods: forty BALB/c mice were randomly divided into 5 days stress group and 15 days restraint stress group. The chronic psychological stress model was established with 10 mice in each group. After stress, open field test and Morris water maze test were used to observe the behavior of mice under stress. The thymus, spleen index and lymphocyte number of spleen and mesenteric lymph nodes were measured. The levels of serum IFN- 緯 and IL-4 and the ratio of IL-4 / IFN- 緯 were measured by ELISA method. Results: psychological stress significantly prolonged the incubation period of mice in open field test, and decreased the activity of inquiry (P0.05 and P0.01). Fecal frequency increased P0.05 and P0.01 Morris water labyrinth of mice in the search for platform latency prolonged compared with the control group (P0.05 and P0.01). In the space exploration experiment, the proportion of swimming time in the platform quadrant in the stress group was lower than that in the control group, suggesting that chronic psychological stress could lead to anxiety in the mice. Depression and cognitive impairment. The body weight of the stress group was always inhibited as compared with the control group. The spleen index and the number of lymphocytes in spleen and mesenteric lymph nodes were significantly inhibited in each stress group. The spleen index of 5-day stress group was significantly lower than that of control group on day 10 and 15, while the thymus index was lower than that of control group. The ratio of IL-4 / IFN- 緯 was significantly lower than that of normal controls (P0.05 and P0.01), but there was no significant difference in IL-4 level. These results suggest that psychological stress inhibits the body weight and immune function of mice and results in imbalance of Th1/Th2 balance and drift to Th2 cells. The spleen index and lymphocyte IFN- 緯 of spleen and mesenteric lymph node were positively correlated with translattice. There was a negative correlation between the residence time of central lattice and feces, suggesting that the emotional behavior and cognitive impairment induced by stress might be related to the inhibition of immune function. Conclusion: chronic psychological stress can cause the changes of emotional behavior and cognitive function, mainly manifested as emotional anxiety, depression, learning and memory, adaptation, response and other cognitive ability decline. Chronic psychological stress can affect body weight and immune function, leading to body weight change and immune decline, resulting in physical weakness, disease or disease; The changes of emotional behavior and cognitive function caused by psychological stress are related to immune function, therefore, the changes of psychological behavior and immune function may be the main causes of sub-health state.
【學(xué)位授予單位】：山西醫(yī)科大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2006
【分類號(hào)】：R395

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