本文關(guān)鍵詞：大鼠在獲得空間辨別性學(xué)習(xí)記憶時(shí)海馬結(jié)構(gòu)形態(tài)學(xué)可塑性研究　出處：《暨南大學(xué)》2005年碩士論文　論文類型：學(xué)位論文

  更多相關(guān)文章： 空間辨別性學(xué)習(xí)記憶 海馬結(jié)構(gòu) ephrin-A3

【摘要】：探討大鼠在獲得空間辨別性學(xué)習(xí)記憶功能時(shí)其海馬結(jié)構(gòu)的形態(tài)學(xué)可塑性及可塑性與有關(guān)神經(jīng)因子表達(dá)之間的關(guān)系。 方法 水迷宮訓(xùn)練建立空間辨別性學(xué)習(xí)記憶動(dòng)物模型后,用Timm染色方法、免疫組織化學(xué)方法和Western-blot方法檢測模型大鼠、游水大鼠和對照大鼠海馬結(jié)構(gòu)神經(jīng)形念學(xué)可塑性反應(yīng)—神經(jīng)纖維出芽、以及與神經(jīng)可塑性有關(guān)的導(dǎo)向分子ephrin-A3表達(dá)差異。 結(jié)果 1.建立空間辨別性學(xué)習(xí)記憶動(dòng)物模型的結(jié)果:大鼠經(jīng)過水迷宮訓(xùn)練到第7d時(shí),其平均潛伏期、平均運(yùn)行時(shí)間以及正確率三項(xiàng)指標(biāo)即可到達(dá)規(guī)定指標(biāo); 2.各組大鼠海馬結(jié)構(gòu)可塑性的結(jié)果:(1)模型組大鼠海馬結(jié)構(gòu)內(nèi)Timm染色顯示出芽的神經(jīng)纖維主要分布在CA3區(qū)多形層,并且出芽反應(yīng)以14d最旺盛;9只模型大鼠中有8只顯示神經(jīng)纖維出芽(88.8%);(2)9只游水組大鼠中有2只在海馬CA3區(qū)多形層分布著出芽的神經(jīng)纖維(22.2%),7只大鼠未見出芽反應(yīng);(3)對照組9只大鼠均未見海馬CA3區(qū)多形層有出芽的神經(jīng)纖維分布。 3.各組大鼠海馬結(jié)構(gòu)內(nèi)ephrin-A3的定位和定量結(jié)果:在定位方面,模型組、游水組和對照組大鼠海馬結(jié)構(gòu)各亞區(qū)和各層均可見ephrin-A3免疫反應(yīng)陽性產(chǎn)物分布,但模型組大鼠海馬CA3區(qū)輻射層反應(yīng)尤為明顯。在定量方面,模型組大鼠海馬結(jié)構(gòu)內(nèi)ephrin-A3含量明顯高于游水組和對照組的(P0.01),游水組的比對照組的亦高,差異有顯著性(P0.01);模型組內(nèi)14d和21d比7d高,差異有顯著性(P0.01)。 結(jié)論 1.用水迷宮訓(xùn)練大鼠,可以建立空間辨別性學(xué)習(xí)記憶模型; 2.獲空間辨別性學(xué)習(xí)記憶功能的大鼠海馬結(jié)構(gòu)發(fā)生了神經(jīng)纖維出芽可塑性: 3.ephrin-A3免疫反應(yīng)陽性物與海馬結(jié)構(gòu)神經(jīng)纖維出芽有相關(guān)關(guān)系。
[Abstract]:To investigate the relationship between morphological plasticity and plasticity of hippocampal structure and the expression of related neurofactors during the acquisition of spatial discriminant learning and memory function in rats. Method After establishing the animal model of spatial discriminant learning and memory by water maze training, the model rats were detected by Timm staining, immunohistochemistry and Western-blot. The neuronal plasticity of hippocampal formation in swimming rats and control rats was different from that in control rats. The neuronal fibers sprout and the ephrin-A3 expression of guiding molecules related to neural plasticity were different between the swimming rats and the control rats. Results 1. The results of establishing the animal model of spatial discriminant learning and memory: the average latency of the rats after water maze training was 7 days. The average running time and the correct rate can reach the specified index. 2. The results of the plasticity of hippocampal formation in each group: 1) the Timm staining in the hippocampal structure of rats in the model group showed that the budding nerve fibers were mainly distributed in the polymorphic layer of the CA3 area. The sprout reaction was the strongest in 14 days. Of the 9 model rats, 8 showed nerve fiber sprouting and 88. 8%. Two of 9 rats in the swimming group had no budding reaction in the polymorphic layer of hippocampal CA3. 3) in the control group, no budding nerve fibers were found in the polymorphic layer of the hippocampal CA3 region. 3. Localization and quantitative results of ephrin-A3 in hippocampal formation of rats in each group: in terms of localization, model group. The distribution of ephrin-A3 immunoreactive products was observed in all subregions and layers of hippocampal formation of rats in swimming group and control group, but the radiation layer reaction in CA3 area of hippocampus in model group was more obvious. The content of ephrin-A3 in the hippocampal structure of the model group was significantly higher than that of the swimming group and the control group (P 0.01), and that of the swimming group was also higher than that of the control group (P 0.01). In the model group, 14 d and 21 d were higher than 7 d, the difference was significant (P 0.01). Conclusion 1. The model of spatial discriminative learning and memory can be established by training rats with water maze. 2. Neural fiber budding plasticity occurred in the hippocampal structure of rats with spatial discriminant learning and memory function. 3. The immunoreactive substance of ephrin-A 3 was correlated with the sprouting of hippocampal nerve fibers.
【學(xué)位授予單位】：暨南大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2005
【分類號(hào)】：R322

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