本文關(guān)鍵詞：bFGF調(diào)節(jié)人內(nèi)皮細(xì)胞ICAM-1表達(dá)的研究　出處：《中國(guó)醫(yī)科大學(xué)》2006年碩士論文　論文類型：學(xué)位論文

  更多相關(guān)文章： 創(chuàng)傷修復(fù) 堿性成纖維細(xì)胞生長(zhǎng)因子 內(nèi)皮細(xì)胞 ICAM-1

【摘要】：bFGF調(diào)節(jié)人內(nèi)皮細(xì)胞ICAM-1表達(dá)的研究 前言 創(chuàng)傷是廣泛危害人類的“發(fā)達(dá)社會(huì)疾病”,深入研究創(chuàng)傷后的組織修復(fù)問題依然是醫(yī)學(xué)領(lǐng)域的重點(diǎn)。創(chuàng)傷修復(fù)是一個(gè)復(fù)雜而有序的生物學(xué)過程,涉及多種炎癥細(xì)胞、修復(fù)細(xì)胞、炎癥介質(zhì)、生長(zhǎng)因子和細(xì)胞外基質(zhì)等成分的共同參與。在組織修復(fù)研究中,各種生長(zhǎng)因子在其中的作用及調(diào)控機(jī)制成'為現(xiàn)代創(chuàng)傷修復(fù)研究的熱點(diǎn)。成纖維細(xì)胞生長(zhǎng)因子(FGF),尤其是堿性成纖維細(xì)胞生長(zhǎng)因子(bFGF)具有廣泛的生物學(xué)活性,是傷口愈合過程中所有相關(guān)細(xì)胞的促有絲分裂原、化學(xué)趨化及調(diào)節(jié)蛋白,可以影響創(chuàng)傷修復(fù)的整個(gè)過程。目前有關(guān)于bFGF促進(jìn)內(nèi)皮細(xì)胞(EC)增殖、調(diào)節(jié)EC蛋白表達(dá)、分泌,促進(jìn)血管新生、肉芽組織形成方面的研究報(bào)道,但有關(guān)bFGF調(diào)節(jié)內(nèi)皮細(xì)胞黏附分子表達(dá),影響早期炎癥反應(yīng)的研究則報(bào)道很少,對(duì)EC細(xì)胞間黏附分子-1(ICAM-1)表達(dá)的影響,國(guó)內(nèi)尚未見報(bào)道。 本實(shí)驗(yàn)通過研究bFGF調(diào)節(jié)人臍靜脈內(nèi)皮細(xì)胞(HUVEC)ICAM-1表達(dá)的時(shí)間效應(yīng),及糖皮質(zhì)激素地塞米松(Dex)對(duì)bFGF調(diào)節(jié)ICAM-1表達(dá)的影響,來闡述bFGF影響修復(fù)早期炎癥反應(yīng),進(jìn)而促進(jìn)創(chuàng)傷修復(fù)的可能機(jī)制。 方法 1.EC的培養(yǎng):采用本室改進(jìn)的Jaffe氏法進(jìn)行HUVEC培養(yǎng)。經(jīng)免疫熒光檢測(cè)Ⅷ因子抗原陽(yáng)性,鑒定為血管內(nèi)皮細(xì)胞,采用1-3代EC進(jìn)行實(shí)驗(yàn),待細(xì)胞達(dá)亞融合狀態(tài)后,換無血清培養(yǎng)液24h,之后施加各實(shí)驗(yàn)因素。2.HUVEC ICAM-1表達(dá)的測(cè)定:實(shí)驗(yàn)分組:(1)不同時(shí)間組:對(duì)照組(無血清DMEM);實(shí)驗(yàn)組:bFGF(500ng/ml)12h、16h、24h、48h組(2)不同因素組(16h):對(duì)照組(無血清DMEM);bFGF(500ng/ml)、Dex(0.1μmol/L)、bFGF+Dex組。采用免疫細(xì)胞化學(xué)法(SP法)及流式細(xì)胞儀檢測(cè)法分析EC
[Abstract]:Regulation of ICAM-1 expression in Human Endothelial cells by bFGF Foreword Trauma is a "developed social disease" that harms human beings extensively. It is still the focus of medical field to study the tissue repair problem after trauma. Wound repair is a complicated and orderly biological process. Involved in a variety of inflammatory cells repair cells inflammatory mediators growth factors and extracellular matrix and other components involved in tissue repair research. The role and regulatory mechanism of various growth factors are the focus of modern wound repair research. Fibroblast growth factor (FGFs). In particular, basic fibroblast growth factor (bFGF) has a wide range of biological activities, and is a mitogen, chemotaxis and regulatory protein of all the related cells during wound healing. BFGF promotes endothelial cell proliferation, regulates the expression and secretion of EC protein, promotes angiogenesis and granulation tissue formation. However, there are few reports about the effect of bFGF on the expression of adhesion molecules in endothelial cells, and on the expression of intercellular adhesion molecule-1 (ICAM-1) in EC. No reports have been reported at home. The aim of this study was to investigate the effect of bFGF on the expression of ICAM-1 in human umbilical vein endothelial cells (HUVECs). The effect of glucocorticoid dexamethasone Dexon on the expression of ICAM-1 by bFGF was used to elucidate the possible mechanism of bFGF affecting the early inflammatory response and promoting wound repair. Method 1. EC culture: HUVEC culture was carried out by modified Jaffe's method in our laboratory. Factor 鈪，

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