本文關(guān)鍵詞：bFGF調(diào)節(jié)人內(nèi)皮細胞ICAM-1表達的研究　出處：《中國醫(yī)科大學》2006年碩士論文　論文類型：學位論文

  更多相關(guān)文章： 創(chuàng)傷修復 堿性成纖維細胞生長因子 內(nèi)皮細胞 ICAM-1

【摘要】：bFGF調(diào)節(jié)人內(nèi)皮細胞ICAM-1表達的研究 前言 創(chuàng)傷是廣泛危害人類的“發(fā)達社會疾病”,深入研究創(chuàng)傷后的組織修復問題依然是醫(yī)學領(lǐng)域的重點。創(chuàng)傷修復是一個復雜而有序的生物學過程,涉及多種炎癥細胞、修復細胞、炎癥介質(zhì)、生長因子和細胞外基質(zhì)等成分的共同參與。在組織修復研究中,各種生長因子在其中的作用及調(diào)控機制成'為現(xiàn)代創(chuàng)傷修復研究的熱點。成纖維細胞生長因子(FGF),尤其是堿性成纖維細胞生長因子(bFGF)具有廣泛的生物學活性,是傷口愈合過程中所有相關(guān)細胞的促有絲分裂原、化學趨化及調(diào)節(jié)蛋白,可以影響創(chuàng)傷修復的整個過程。目前有關(guān)于bFGF促進內(nèi)皮細胞(EC)增殖、調(diào)節(jié)EC蛋白表達、分泌,促進血管新生、肉芽組織形成方面的研究報道,但有關(guān)bFGF調(diào)節(jié)內(nèi)皮細胞黏附分子表達,影響早期炎癥反應的研究則報道很少,對EC細胞間黏附分子-1(ICAM-1)表達的影響,國內(nèi)尚未見報道。 本實驗通過研究bFGF調(diào)節(jié)人臍靜脈內(nèi)皮細胞(HUVEC)ICAM-1表達的時間效應,及糖皮質(zhì)激素地塞米松(Dex)對bFGF調(diào)節(jié)ICAM-1表達的影響,來闡述bFGF影響修復早期炎癥反應,進而促進創(chuàng)傷修復的可能機制。 方法 1.EC的培養(yǎng):采用本室改進的Jaffe氏法進行HUVEC培養(yǎng)。經(jīng)免疫熒光檢測Ⅷ因子抗原陽性,鑒定為血管內(nèi)皮細胞,采用1-3代EC進行實驗,待細胞達亞融合狀態(tài)后,換無血清培養(yǎng)液24h,之后施加各實驗因素。2.HUVEC ICAM-1表達的測定:實驗分組:(1)不同時間組:對照組(無血清DMEM);實驗組:bFGF(500ng/ml)12h、16h、24h、48h組(2)不同因素組(16h):對照組(無血清DMEM);bFGF(500ng/ml)、Dex(0.1μmol/L)、bFGF+Dex組。采用免疫細胞化學法(SP法)及流式細胞儀檢測法分析EC
[Abstract]:Regulation of ICAM-1 expression in Human Endothelial cells by bFGF Foreword Trauma is a "developed social disease" that harms human beings extensively. It is still the focus of medical field to study the tissue repair problem after trauma. Wound repair is a complicated and orderly biological process. Involved in a variety of inflammatory cells repair cells inflammatory mediators growth factors and extracellular matrix and other components involved in tissue repair research. The role and regulatory mechanism of various growth factors are the focus of modern wound repair research. Fibroblast growth factor (FGFs). In particular, basic fibroblast growth factor (bFGF) has a wide range of biological activities, and is a mitogen, chemotaxis and regulatory protein of all the related cells during wound healing. BFGF promotes endothelial cell proliferation, regulates the expression and secretion of EC protein, promotes angiogenesis and granulation tissue formation. However, there are few reports about the effect of bFGF on the expression of adhesion molecules in endothelial cells, and on the expression of intercellular adhesion molecule-1 (ICAM-1) in EC. No reports have been reported at home. The aim of this study was to investigate the effect of bFGF on the expression of ICAM-1 in human umbilical vein endothelial cells (HUVECs). The effect of glucocorticoid dexamethasone Dexon on the expression of ICAM-1 by bFGF was used to elucidate the possible mechanism of bFGF affecting the early inflammatory response and promoting wound repair. Method 1. EC culture: HUVEC culture was carried out by modified Jaffe's method in our laboratory. Factor 鈪，

本文編號：1370353