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  本文關(guān)鍵詞：小鼠吸入性鼠疫模型的組織病理學(xué)和鼠疫耶爾森氏菌重要毒力相關(guān)基因體內(nèi)轉(zhuǎn)錄水平的研究　出處：《吉林大學(xué)》2006年博士論文　論文類型：學(xué)位論文

  更多相關(guān)文章： 鼠疫耶爾森菌 吸入性感染模型 組織病理學(xué) 毒力相關(guān)基因 體內(nèi)表達

【摘要】：鼠疫耶爾森氏菌(Yersina pestis)是烈性傳染病鼠疫的病原體。鼠疫曾給人類帶來了巨大的災(zāi)難,而且仍是21世紀(jì)的瘟疫。 為了揭示吸入性鼠疫的發(fā)生、發(fā)展過程,探討鼠疫菌致病機制,本研究建立了小鼠吸入性鼠疫模型,組織病理切片、光學(xué)及電子顯微鏡觀察結(jié)果顯示,鼠疫菌吸入后在小鼠可誘導(dǎo)產(chǎn)生肺鼠疫型和非肺鼠疫型鼠疫發(fā)生;肺鼠疫的最基本的病理改變?yōu)槌鲅�、炎細胞浸潤、肺組織壞死、細菌大量增殖及晚期多臟器損傷;生存環(huán)境可能導(dǎo)致鼠疫菌形態(tài)及超微結(jié)構(gòu)的改變,肺泡腔中菌細胞的表面被覆菌毛和F1抗原,肺間質(zhì)中,在高電子密度的菌體周圍可見均勻、致密較低電子密度的莢膜;在感染的過程中,除巨噬細胞和中性粒細胞外,淋巴系統(tǒng)也受到嚴(yán)重的損傷,感染晚期脾、胸腺淋巴細胞發(fā)生壞死和凋亡;免疫組化染色結(jié)果提示,鼠疫菌5種毒力相關(guān)蛋白,F1、LcrV、LcrG、Pla、pH6抗原在宿主體內(nèi)均表現(xiàn)為菌體表面可檢測到的表達;實時定量RT-PCR結(jié)果顯示,其相應(yīng)毒力基因在感染的不同時間及組織中呈差異性表達,這種表達的差異性體現(xiàn)了鼠疫病程發(fā)展過程中鼠疫菌與宿主之間的相互作用,在鼠疫菌致病機制中具有重要意義。 本研究為鼠疫菌基因組學(xué)研究奠定了理論和實踐基礎(chǔ),對鼠疫預(yù)防、治療及致病機制研究具有重要意義。
[Abstract]:The plague, Jerson Prand (Yersina pestis), is the pathogen of the plague of the strong infectious disease. The plague had brought great disaster to mankind, and was still the plague in twenty-first Century.
In order to reveal the inhalation plague occurrence, development process, to explore the pathogenic mechanism of Yersinia pestis, this study established a mouse model of inhalation plague, histopathology, optical and electron microscopy showed that Yersinia pestis after inhalation in mice can induce lung plague and pneumonic plague; plague the most basic the pathological changes of pulmonary hemorrhage, inflammatory cell infiltration, tissue necrosis, bacterial proliferation and late multiple organ injury; living environment may cause the plague bacteria morphology and ultrastructure changes of surface coated fimbriae and F1 antigen in bacterial cells of alveoli, interstitial lung, around the high electron density was visible uniform. Compact low electron density capsule; during the course of infection in macrophages and neutrophils, the lymphatic system also suffered serious damage, infection of advanced spleen, lymphocyte necrosis and thymus Apoptosis; immunohistochemistry staining showed that related proteins of Yersinia pestis virulence, 5 F1, LcrV, LcrG, Pla, pH6 antigen in the host cell surface expression were detected; real-time quantitative RT-PCR results showed that the different time and the corresponding virulence genes in infection showed differential expression this expression, the difference reflects the interaction between Yersinia pestis and host of plague in the course of the development process, plays an important role in the pathogenesis of Yersinia pestis.
This study has laid a theoretical and practical basis for the genomics of Yersinia pestis, and is of great significance to the prevention, treatment and pathogenesis of plague.

【學(xué)位授予單位】：吉林大學(xué)
【學(xué)位級別】：博士
【學(xué)位授予年份】：2006
【分類號】：R363

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相關(guān)期刊論文 前1條

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本文編號：1361665