本文關鍵詞：小鼠吸入性鼠疫模型的組織病理學和鼠疫耶爾森氏菌重要毒力相關基因體內轉錄水平的研究　出處：《吉林大學》2006年博士論文　論文類型：學位論文

  更多相關文章： 鼠疫耶爾森菌 吸入性感染模型 組織病理學 毒力相關基因 體內表達

【摘要】：鼠疫耶爾森氏菌(Yersina pestis)是烈性傳染病鼠疫的病原體。鼠疫曾給人類帶來了巨大的災難,而且仍是21世紀的瘟疫。 為了揭示吸入性鼠疫的發(fā)生、發(fā)展過程,探討鼠疫菌致病機制,本研究建立了小鼠吸入性鼠疫模型,組織病理切片、光學及電子顯微鏡觀察結果顯示,鼠疫菌吸入后在小鼠可誘導產生肺鼠疫型和非肺鼠疫型鼠疫發(fā)生;肺鼠疫的最基本的病理改變?yōu)槌鲅�、炎細胞浸潤、肺組織壞死、細菌大量增殖及晚期多臟器損傷;生存環(huán)境可能導致鼠疫菌形態(tài)及超微結構的改變,肺泡腔中菌細胞的表面被覆菌毛和F1抗原,肺間質中,在高電子密度的菌體周圍可見均勻、致密較低電子密度的莢膜;在感染的過程中,除巨噬細胞和中性粒細胞外,淋巴系統(tǒng)也受到嚴重的損傷,感染晚期脾、胸腺淋巴細胞發(fā)生壞死和凋亡;免疫組化染色結果提示,鼠疫菌5種毒力相關蛋白,F1、LcrV、LcrG、Pla、pH6抗原在宿主體內均表現為菌體表面可檢測到的表達;實時定量RT-PCR結果顯示,其相應毒力基因在感染的不同時間及組織中呈差異性表達,這種表達的差異性體現了鼠疫病程發(fā)展過程中鼠疫菌與宿主之間的相互作用,在鼠疫菌致病機制中具有重要意義。 本研究為鼠疫菌基因組學研究奠定了理論和實踐基礎,對鼠疫預防、治療及致病機制研究具有重要意義。
[Abstract]:The plague, Jerson Prand (Yersina pestis), is the pathogen of the plague of the strong infectious disease. The plague had brought great disaster to mankind, and was still the plague in twenty-first Century.
In order to reveal the inhalation plague occurrence, development process, to explore the pathogenic mechanism of Yersinia pestis, this study established a mouse model of inhalation plague, histopathology, optical and electron microscopy showed that Yersinia pestis after inhalation in mice can induce lung plague and pneumonic plague; plague the most basic the pathological changes of pulmonary hemorrhage, inflammatory cell infiltration, tissue necrosis, bacterial proliferation and late multiple organ injury; living environment may cause the plague bacteria morphology and ultrastructure changes of surface coated fimbriae and F1 antigen in bacterial cells of alveoli, interstitial lung, around the high electron density was visible uniform. Compact low electron density capsule; during the course of infection in macrophages and neutrophils, the lymphatic system also suffered serious damage, infection of advanced spleen, lymphocyte necrosis and thymus Apoptosis; immunohistochemistry staining showed that related proteins of Yersinia pestis virulence, 5 F1, LcrV, LcrG, Pla, pH6 antigen in the host cell surface expression were detected; real-time quantitative RT-PCR results showed that the different time and the corresponding virulence genes in infection showed differential expression this expression, the difference reflects the interaction between Yersinia pestis and host of plague in the course of the development process, plays an important role in the pathogenesis of Yersinia pestis.
This study has laid a theoretical and practical basis for the genomics of Yersinia pestis, and is of great significance to the prevention, treatment and pathogenesis of plague.

【學位授予單位】：吉林大學
【學位級別】：博士
【學位授予年份】：2006
【分類號】：R363

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相關期刊論文 前1條

1 劉振才,海榮,李富忠,李超,叢顯斌,高崇華,汪立茂,魏柏青,陳洪艦,陳虹,于曉濤,李存香,石映祥,許光榮,李光清,吳國康,嚴冬麗,李敏,曹淑蘭,張春華,魏建春,蔡虹;青藏高原青海田鼠鼠疫自然疫源地的發(fā)現與研究[J];中國地方病防治雜志;2001年06期

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