目的:對(duì)比分析脾臟、腹腔和骨髓源性巨噬細(xì)胞（SPMs、PCMs和BMs）在安靜及極化狀態(tài)下的差異。創(chuàng)新點(diǎn):首次對(duì)比分析脾臟、腹腔和骨髓源性巨噬細(xì)胞在安靜（M0）及極化狀態(tài)（M1和M2）下的特征。方法:通過小鼠脾臟研磨及單細(xì)胞貼壁獲得脾源性巨噬細(xì)胞;腹腔灌洗及細(xì)胞貼壁獲得腹腔源性巨噬細(xì)胞;骨髓貼壁細(xì)胞在巨噬細(xì)胞集落刺激因子體外刺激下培養(yǎng)7天獲得骨髓源性巨噬細(xì)胞。三種細(xì)胞即為M0型巨噬細(xì)胞,M0在干擾素及脂多糖刺激下獲得M1型巨噬細(xì)胞,M0在白介素4（IL-4）刺激下獲得M2型巨噬細(xì)胞。通過流式細(xì)胞儀分析三種類型巨噬細(xì)胞在三種狀態(tài)下的Ⅱ類主要組織相容性復(fù)合體（MHC Ⅱ）和CD86表達(dá)差異。通過實(shí)時(shí)熒光定量聚合酶鏈?zhǔn)椒磻?yīng)（q PCR）檢測(cè)腫瘤壞死因子α（TNF-α）、白介素1β（IL-1β）、甘露糖受體（MR）和類幾丁質(zhì)酶3樣分子（Ym1）的表達(dá)變化。結(jié)論:骨髓貼壁細(xì)胞培養(yǎng)能獲得最大量的同源巨噬細(xì)胞（圖1和2）,但表型偏向于M2型巨噬細(xì)胞（圖3和4）。三種巨噬細(xì)胞均能極化為M1和M2型巨噬細(xì)胞（圖5）,其中SPMs具有更強(qiáng)的M1型極化能力,而M2型極化能力之間未見明顯差異（圖6）。綜上所述... 

【文章來(lái)源】：Journal of Zhejiang University-Science B（Biomedicine & Biotechnology）. 2017,18(12)SCICSCD

【文章頁(yè)數(shù)】：9 頁(yè)

【文章目錄】：
1 Introduction
2 Materials and methods
    2.1 Experimental animals
    2.2 Macrophage isolation and polarization
    2.3 Flow cytometry
    2.4 Real-time PCR analysis
    2.5 Statistical analysis
3 Results
    3.1 Macrophage yield
    3.2 Macrophage heterogeneity
    3.3 BMs were partially polarized to M2 and SPMs showed M1 polarization
    3.4 All three types of macrophages showed the capacity to polarize into M1 and M2 phenotypes
    3.5 All three types of macrophages showed the capacity to polarize into M1 and M2 phenotypes
4 Discussion


【參考文獻(xiàn)】：
期刊論文
[1]巨噬細(xì)胞產(chǎn)生的白介素-10降低乳腺癌的化療療效（英文）[J]. Xinguo JIANG.  Journal of Zhejiang University-Science B（Biomedicine & Biotechnology）. 2015(01)
[2]Expression and preliminary functional analysis of Siglec-F on mouse macrophages[J]. Yin-he FENG, Hui MAO(Department of Respiratory Medicine, West China Hospital, Sichuan University, Chengdu 610041, China).  Journal of Zhejiang University-Science B（Biomedicine & Biotechnology）. 2012(05)



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