自氧化偶氮甲烷（AOM）-硫酸葡聚糖硫酸鈉（DSS）誘導(dǎo)腫瘤用于研究炎癥相關(guān)或散發(fā)性結(jié)直腸癌（CRC）以來,數(shù)種AOM、DSS處理方式被報(bào)導(dǎo)。本文優(yōu)化了AOM-DSS誘導(dǎo)C57BL/6小鼠散發(fā)性結(jié)直腸癌模型的方法。利用100μl消毒注射器按照需要?jiǎng)┝恳淮涡愿骨蛔⑸溥m當(dāng)濃度AOM,DSS處理7天并每天更換新鮮的DSS液體。我們共誘導(dǎo)超過100只小鼠,AOM處理后8至20周時(shí),發(fā)現(xiàn)每只小鼠均被誘導(dǎo)發(fā)生結(jié)直腸腫瘤。腫瘤分布以結(jié)直腸遠(yuǎn)端居多,與人類結(jié)直腸癌相似。采用此方法建模的每只小鼠的成瘤數(shù)量較多、鼠間腫瘤數(shù)量差異較小。此AOM-DSS模型中的腫瘤病變涵蓋了瘤前病變、腺瘤性病變、腺瘤以及腺癌。本改良的AOM-DSS結(jié)直腸癌建模方法具有簡單、經(jīng)濟(jì)、成瘤率高等優(yōu)點(diǎn)。 

【文章來源】：Chinese Medical Sciences Journal. 2019,34(04)CSCD

【文章頁數(shù)】：9 頁

【文章目錄】：
INTRODUCTION
    Background
APPLICATION AND VALUE
EXPERIMENTAL DESIGN
    Mice selection
    AOM treatment
    DSS treatment
    Waiting time period
    Monitoring body weight
MATERIALS
    Regents
    Equipments
PROCEDURES
    Mouse preparations for AOM-DSS induced colorectal tumorigenesis
    AOM stock solution preparation
    AOM treatment
    The treatment interval
    DSS treatment
    Waiting period
    Colon collection
TIMING
ANTICIPATING RESULTS
    Conflict of interest statement


【參考文獻(xiàn)】：
期刊論文
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[2]Dextran sulfate sodium-induced colitis-associated neoplasia:a promising model for the development of chemopreventive interventions[J]. Margie Lee CLAPPER,Harry Stanley COOPER,Wen-Chi Lee CHANG.  Acta Pharmacologica Sinica. 2007(09)



本文編號(hào)：3473538