【摘要】：目的 探究富血小板纖維蛋白（PRF）凝膠中的細(xì)胞因子對SD大鼠骨髓間充質(zhì)干細(xì)胞體外成骨成脂定向誘導(dǎo)分化的影響，為組織工程種子細(xì)胞體外培養(yǎng)和口腔種植臨床應(yīng)用提供理論依據(jù)和實踐指導(dǎo)。 方法 取SD大鼠股骨和脛骨骨髓液，貼壁法培養(yǎng)。傳代后進(jìn)行增殖，，在實驗組培養(yǎng)基中加入PRF凝膠，對照組不加。培養(yǎng)1-7天進(jìn)行MTT檢測細(xì)胞增殖活性。同時分別進(jìn)行成骨細(xì)胞和脂肪細(xì)胞誘導(dǎo)，7d后去除殘余的PRF凝膠，培養(yǎng)14d成骨誘導(dǎo)組進(jìn)行茜素紅染色、礦化結(jié)節(jié)半定量分析、ALP檢測，骨鈣素含量檢測。成脂誘導(dǎo)組進(jìn)行油紅o染色和定量分析。 結(jié)果 MTT檢測顯示1~3天內(nèi)，PRF組和對照組無顯著性差異，4~7天開始，PRF組吸光值大于對照組。成骨誘導(dǎo)茜素紅染色觀察PRF組紅色礦化結(jié)節(jié)數(shù)量較對照組為多。骨鈣素檢測顯示PRF組OD值大于對照組（P0.05），ALP檢測、半定量分析顯示PRF組A值均大于對照組（P0.05）。油紅O染色顯示PRF組脂滴含量大于對照組，定量分析PRF組A值對照組（P0.05） 結(jié)論 PRF凝膠所含細(xì)胞因子可促進(jìn)大鼠骨髓間充質(zhì)干細(xì)胞增殖，對成骨分化和成脂分化同樣有促進(jìn)作用。PRF凝膠可作為骨組織工程種子細(xì)胞體外培養(yǎng)生長因子源。
[Abstract]:Purpose To investigate the effect of cytokines in platelet-rich fibrin (PRF) gel on the differentiation of bone marrow-derived mesenchymal stem cells in vitro and to induce differentiation of bone marrow-derived mesenchymal stem cells in vitro, and to provide the theoretical basis and practical means for the in vitro culture of the tissue engineering seed cells and the clinical application of oral planting. Conductors. Methods SD rat femur and tibia bone marrow fluid were obtained. the culture was carried out after passage, and the propagation was carried out after passage, and the PRF gel was added to the culture medium of the experimental group, The control group was not cultured for 1-7 days and the MTT assay was fine. Cell proliferation was induced by osteoblasts and adipocytes, and the residual PRF gel was removed at 7 days. After 7 days, the residual PRF gel was removed, and the 14-day osteogenic induction group was cultured for fluorescein red staining, semi-quantitative analysis of mineralized nodules, ALP detection, and bone calcium. The detection of the content of the pigment. The fat-forming induction group was stained with oil red o. and The results showed that there was no significant difference between the PRF group and the control group within 1 to 3 days after the MTT test. The light absorption value of the group was greater than that of the control group. The red mineralization of the PRF group was observed by osteogenic induction. The results showed that the OD value of the PRF group was greater than that of the control group (P0.05), the ALP test and the semi-quantitative analysis showed that the value of the group A in the PRF group was greater than that of the control group (P0.05). Group A (P0.05). The oil red O staining showed that the lipid drop in the PRF group was greater than that of the control group, and the value of the PRF group A was quantitatively analyzed. control Conclusion Cytokine in the PRF gel can promote the proliferation of rat bone marrow-derived mesenchymal stem cells. The PRF gel can be used as bone tissue engineering.
【學(xué)位授予單位】：遼寧醫(yī)學(xué)院
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2012
【分類號】：R329

【參考文獻(xiàn)】

相關(guān)期刊論文 前6條

1 王貞;夏文杰;葉欣;羅廣平;戎霞;付涌水;;人血小板裂解液對骨髓間質(zhì)干細(xì)胞成骨成脂分化的影響[J];中國輸血雜志;2011年10期

2 馬寧;徐文洲;張正;鄭義;張莉;;GTR聯(lián)合PRF修復(fù)牙周炎骨缺損1例報告[J];吉林大學(xué)學(xué)報(醫(yī)學(xué)版);2010年04期

3 周延民;;Choukroun's PRF在種植臨床的應(yīng)用[J];中國口腔種植學(xué)雜志;2009年02期

4 何琳琳;蘭飛;薛小平;;腫瘤壞死因子-α對小鼠骨髓間充質(zhì)干細(xì)胞成骨分化影響研究[J];中國生物工程雜志;2011年01期

5 周延民;李琦;儲順禮;李春艷;王宇;;即刻種植間隙充填富血小板纖維蛋白修復(fù)骨缺損2例[J];中國實用口腔科雜志;2009年08期

6 李宇琳;周恒;劉廣鵬;崔磊;;茜素紅半定量測定細(xì)胞基質(zhì)鈣含量的實驗研究[J];組織工程與重建外科雜志;2008年02期

本文編號：2508621