【摘要】：目的 探究富血小板纖維蛋白（PRF）凝膠中的細胞因子對SD大鼠骨髓間充質(zhì)干細胞體外成骨成脂定向誘導分化的影響，為組織工程種子細胞體外培養(yǎng)和口腔種植臨床應用提供理論依據(jù)和實踐指導。 方法 取SD大鼠股骨和脛骨骨髓液，貼壁法培養(yǎng)。傳代后進行增殖，，在實驗組培養(yǎng)基中加入PRF凝膠，對照組不加。培養(yǎng)1-7天進行MTT檢測細胞增殖活性。同時分別進行成骨細胞和脂肪細胞誘導，7d后去除殘余的PRF凝膠，培養(yǎng)14d成骨誘導組進行茜素紅染色、礦化結(jié)節(jié)半定量分析、ALP檢測，骨鈣素含量檢測。成脂誘導組進行油紅o染色和定量分析。 結(jié)果 MTT檢測顯示1~3天內(nèi)，PRF組和對照組無顯著性差異，4~7天開始，PRF組吸光值大于對照組。成骨誘導茜素紅染色觀察PRF組紅色礦化結(jié)節(jié)數(shù)量較對照組為多。骨鈣素檢測顯示PRF組OD值大于對照組（P0.05），ALP檢測、半定量分析顯示PRF組A值均大于對照組（P0.05）。油紅O染色顯示PRF組脂滴含量大于對照組，定量分析PRF組A值對照組（P0.05） 結(jié)論 PRF凝膠所含細胞因子可促進大鼠骨髓間充質(zhì)干細胞增殖，對成骨分化和成脂分化同樣有促進作用。PRF凝膠可作為骨組織工程種子細胞體外培養(yǎng)生長因子源。
[Abstract]:Purpose To investigate the effect of cytokines in platelet-rich fibrin (PRF) gel on the differentiation of bone marrow-derived mesenchymal stem cells in vitro and to induce differentiation of bone marrow-derived mesenchymal stem cells in vitro, and to provide the theoretical basis and practical means for the in vitro culture of the tissue engineering seed cells and the clinical application of oral planting. Conductors. Methods SD rat femur and tibia bone marrow fluid were obtained. the culture was carried out after passage, and the propagation was carried out after passage, and the PRF gel was added to the culture medium of the experimental group, The control group was not cultured for 1-7 days and the MTT assay was fine. Cell proliferation was induced by osteoblasts and adipocytes, and the residual PRF gel was removed at 7 days. After 7 days, the residual PRF gel was removed, and the 14-day osteogenic induction group was cultured for fluorescein red staining, semi-quantitative analysis of mineralized nodules, ALP detection, and bone calcium. The detection of the content of the pigment. The fat-forming induction group was stained with oil red o. and The results showed that there was no significant difference between the PRF group and the control group within 1 to 3 days after the MTT test. The light absorption value of the group was greater than that of the control group. The red mineralization of the PRF group was observed by osteogenic induction. The results showed that the OD value of the PRF group was greater than that of the control group (P0.05), the ALP test and the semi-quantitative analysis showed that the value of the group A in the PRF group was greater than that of the control group (P0.05). Group A (P0.05). The oil red O staining showed that the lipid drop in the PRF group was greater than that of the control group, and the value of the PRF group A was quantitatively analyzed. control Conclusion Cytokine in the PRF gel can promote the proliferation of rat bone marrow-derived mesenchymal stem cells. The PRF gel can be used as bone tissue engineering.
【學位授予單位】：遼寧醫(yī)學院
【學位級別】：碩士
【學位授予年份】：2012
【分類號】：R329

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