【摘要】：目的探討CXC亞族趨化因子受體3（CXCR3）及其配體I-TAC在免疫性血小板減少性紫癜（ITP）發(fā)病機制中的作用以及免疫抑制劑治療后的水平。 方法應用酶聯(lián)免疫吸附試驗（ELISA）和實時定量PCR（RT-PCR）法，對30例初診及復發(fā)ITP患者治療前、18例治療有效患者和24例健康人的血漿γ-干擾素（IFN-γ）、I-TAC的含量和外周血單個核細胞（PBMNC）CXCR3的相對表達進行檢測。 結果（1）治療前ITP患者血漿中IFN-γ的含量（71.45±17.62）pg/ml明顯高于治療有效組（36.9±14.9）pg/ml與正常對照組（25.28±12.85）pg/ml（P0.05），ITP治療有效組血漿IFN-γ的含量仍高于正常對照組（P0.05）。（2）治療前ITP患者血漿中I-TAC的含量（455.56±144.70）pg/ml與治療有效組（488.24±164.70）pg/ml及正常對照組（382.97±167.43）pg/ml相比，三者之間無統(tǒng)計學意義（P0.05）。（3）與正常對照（相對表達中位數(shù)為0.12倍）（0.04，，0.28）相比，治療前（相對表達中位數(shù)為6.76倍）（3.03，37.00）與治療有效組（相對表達中位數(shù)為1.76倍）（0.45，14.18）ITP患者PBMNC高表達CXCR3mRNA（P0.05），治療有效后CXCR3表達降低，但與治療前組對比差異無統(tǒng)計學意義（P0.05）。 結論ITP患者表現(xiàn)Th1型細胞因子優(yōu)勢同時，CXCR3+細胞可能通過趨化機制在ITP免疫機制中發(fā)揮一定的作用。另外，免疫抑制治療對該作用軸有一定的影響。
[Abstract]:Objective to investigate the role of CXC subfamily chemokine receptor 3 (CXCR3) and its ligand I-TAC in the pathogenesis of immune thrombocytopenic purpura (ITP) and the level of immunosuppressive agents. Methods enzyme-linked immunosorbent assay (ELISA) and real-time quantitative PCR (RT-PCR) were used to detect the content of plasma interferon-gamma (IFN- 緯), I-TAC and the relative expression of (PBMNC) CXCR3 in peripheral blood mononuclear cells in 30 patients with newly diagnosed and recurrent ITP, 18 patients with effective treatment and 24 healthy controls. Results (1) the content of IFN- 緯 in plasma of patients with ITP before treatment (41.45 鹵17.62) pg/ml was significantly higher than that of effective group (36.9 鹵14.9) pg/ml and normal control group (25.28 鹵12.85) pg/ml (P 0.05). The content of plasma IFN- 緯 in the effective group of ITP was still higher than that in the normal control group (P 0.05). (2). The content of I-TAC in the plasma of ITP patients before treatment was (455.56 鹵144.70) pg/ml, which was higher than that of the effective group (488.24 鹵164.70) pg/ml and the normal control group (382.97 鹵167.43) pg/ml. There was no significant difference among the three groups (P 0.05,). (3) compared with the normal control (the median relative expression was 0. 12 times) (0. 04, 0. 28). Before treatment (the median relative expression was 6. 76 times) (3.03, 37. 00) and the therapeutic effective group (1. 76 times) (0. 45,14. 18), the expression of CXCR3mRNA in PBMNC of ITP patients was lower than that of the control group (0. 45, 14. 18), and the expression of CXCR3 was decreased after treatment. However, there was no significant difference between the pre-treatment group and the pre-treatment group (P 0.05). Conclusion ITP patients show Th1 cytokine dominance, and CXCR3 cells may play a role in the immune mechanism of ITP through chemotactic mechanism. In addition, immunosuppressive therapy has a certain effect on the axis of action.
【學位授予單位】：青海大學
【學位級別】：碩士
【學位授予年份】：2012
【分類號】：R392

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