【摘要】：目的研究銅綠假單胞菌分泌物對(duì)中性粒細(xì)胞趨化功能的影響。方法用percoll密度梯度離心法分離小鼠骨髓中性粒細(xì)胞并用FACS檢測(cè)其純度;銅綠假單胞菌PAO1靜置培養(yǎng)5 d后渦旋并離心收集其上清;通過TAXIScan-FL檢測(cè)被膜上清PAO1 d5(PAO1菌株靜置培養(yǎng)第5天)的趨化作用;不同濃度的f MLP所引起的中性粒細(xì)胞趨化運(yùn)動(dòng)及PAO1 d5上清稀釋50倍后的趨化作用;PAO1 d5上清對(duì)f MLP所引起的中性粒細(xì)胞趨化運(yùn)動(dòng)的影響。結(jié)果分離的小鼠骨髓中性粒細(xì)胞純度大于90%;TAXIScan-FL檢測(cè)被膜上清有趨化作用,但是速度及方向上與f MLP及LTB4有明顯差別(P0.001);高濃度的f MLP對(duì)中性粒細(xì)胞的趨化作用減弱,PAO1 d5稀釋50倍后趨化作用消失;PAO1 d5上清可以抑制f MLP所引起的中性粒細(xì)胞趨化運(yùn)動(dòng)。結(jié)論 PAO1d5被膜上清中存在抑制中性粒細(xì)胞趨化的物質(zhì)。
[Abstract]:Objective to study the effect of Pseudomonas aeruginosa exudates on neutrophil chemotactic function. Methods the bone marrow neutrophils of mice were isolated by percoll density gradient centrifugation and their purity was detected by FACS. After 5 days of static culture of Pseudomonas aeruginosa PAO1, the supernatant was collected by centrifugation; the chemotactic effect of PAO1 d5 (PAO1 strain) was detected by TAXIScan-FL, and the chemotactic activity of neutrophils induced by different concentrations of f MLP and the chemotactic effect of PAO1 d 5 supernatant diluted 50 times. Effect of PAO1 d 5 on neutrophil chemotaxis induced by f MLP. Results the purity of the isolated mouse bone marrow neutrophils was more than 90%. The chemotactic effect of the membrane was detected, but it was significantly different from that of f MLP and LTB4 in speed and direction (P0.001). The chemotactic effect of high concentration of f MLP on neutrophils was weakened, and the chemotactic effect of PAO1 d 5 was disappeared after dilution 50 times. PAO1 d 5 could inhibit the chemotaxis of neutrophils induced by f MLP. Conclusion there is an inhibitor of neutrophil chemotaxis in PAO1d5 capsule.
【作者單位】： 血液病醫(yī)院 中國(guó)科學(xué)院南海海洋研究所
【基金】：國(guó)家自然科學(xué)基金(31271484,31471116)
【分類號(hào)】：R392

【相似文獻(xiàn)】

相關(guān)期刊論文 前8條

1 耿廣鋒;曹善楠;劉鵬;朱海燕;公海艷;相菲;王哲哲;王彤;許元富;王曉雪;羅鴻博;;銅綠假單胞菌分泌物對(duì)中性粒細(xì)胞趨化運(yùn)動(dòng)的抑制功能[J];免疫學(xué)雜志;2016年07期

2 王冰;魏晶;王世儀;孫榮華;張寧;李芳;;蛋白激酶Cζ在人類NSCLC細(xì)胞趨化運(yùn)動(dòng)中的作用[J];大連醫(yī)科大學(xué)學(xué)報(bào);2008年03期

3 施磊;馮影;;細(xì)菌伴侶蛋白Skp引起白細(xì)胞趨化運(yùn)動(dòng)的體內(nèi)實(shí)驗(yàn)研究[J];中國(guó)現(xiàn)代醫(yī)學(xué)雜志;2007年13期

4 施磊;柳斌;關(guān)海霞;徐學(xué)慶;;細(xì)菌伴侶蛋白Skp可通過C5a受體引起白細(xì)胞的趨化運(yùn)動(dòng)[J];中華微生物學(xué)和免疫學(xué)雜志;2005年12期

5 施磊;徐學(xué)慶;李玉姝;關(guān)海霞;;細(xì)菌伴侶蛋白Skp對(duì)C5a受體的作用位點(diǎn)[J];中國(guó)現(xiàn)代醫(yī)學(xué)雜志;2006年09期

6 管茶香,孫秀泓,羅自強(qiáng),秦曉群,張長(zhǎng)青;表皮生長(zhǎng)因子對(duì)肺泡巨噬細(xì)胞趨化性的調(diào)控[J];中國(guó)應(yīng)用生理學(xué)雜志;1998年01期

7 王淑華,胡京紅,盧詠才;人主動(dòng)脈平滑肌細(xì)胞對(duì)淋巴細(xì)胞遷移的影響[J];中華病理學(xué)雜志;1996年01期

8 戴俊,朱一蓓;趨化因子在樹突狀細(xì)胞和T細(xì)胞遷移活化中的作用和意義[J];國(guó)外醫(yī)學(xué)(免疫學(xué)分冊(cè));2005年02期

相關(guān)會(huì)議論文 前2條

1 盧士紅;楊琳;法祥光;;單抗HIM_(82)對(duì)Np質(zhì)膜G蛋白、NEP、CR3的影響[A];第七屆全國(guó)生物膜學(xué)術(shù)討論會(huì)論文摘要匯編[C];1999年

2 丁曉芳;李紅鋼;田永紅;熊錦文;熊承良;;尿激酶型纖溶酶原激活因子在小鼠精-卵通訊中的作用[A];湖北省性學(xué)會(huì)第二屆第二次學(xué)術(shù)年會(huì)論文集[C];2005年

，

本文編號(hào)：2507192