【摘要】：目的研究銅綠假單胞菌分泌物對(duì)中性粒細(xì)胞趨化功能的影響。方法用percoll密度梯度離心法分離小鼠骨髓中性粒細(xì)胞并用FACS檢測(cè)其純度;銅綠假單胞菌PAO1靜置培養(yǎng)5 d后渦旋并離心收集其上清;通過(guò)TAXIScan-FL檢測(cè)被膜上清PAO1 d5(PAO1菌株靜置培養(yǎng)第5天)的趨化作用;不同濃度的f MLP所引起的中性粒細(xì)胞趨化運(yùn)動(dòng)及PAO1 d5上清稀釋50倍后的趨化作用;PAO1 d5上清對(duì)f MLP所引起的中性粒細(xì)胞趨化運(yùn)動(dòng)的影響。結(jié)果分離的小鼠骨髓中性粒細(xì)胞純度大于90%;TAXIScan-FL檢測(cè)被膜上清有趨化作用,但是速度及方向上與f MLP及LTB4有明顯差別(P0.001);高濃度的f MLP對(duì)中性粒細(xì)胞的趨化作用減弱,PAO1 d5稀釋50倍后趨化作用消失;PAO1 d5上清可以抑制f MLP所引起的中性粒細(xì)胞趨化運(yùn)動(dòng)。結(jié)論 PAO1d5被膜上清中存在抑制中性粒細(xì)胞趨化的物質(zhì)。
[Abstract]:Objective to study the effect of Pseudomonas aeruginosa exudates on neutrophil chemotactic function. Methods the bone marrow neutrophils of mice were isolated by percoll density gradient centrifugation and their purity was detected by FACS. After 5 days of static culture of Pseudomonas aeruginosa PAO1, the supernatant was collected by centrifugation; the chemotactic effect of PAO1 d5 (PAO1 strain) was detected by TAXIScan-FL, and the chemotactic activity of neutrophils induced by different concentrations of f MLP and the chemotactic effect of PAO1 d 5 supernatant diluted 50 times. Effect of PAO1 d 5 on neutrophil chemotaxis induced by f MLP. Results the purity of the isolated mouse bone marrow neutrophils was more than 90%. The chemotactic effect of the membrane was detected, but it was significantly different from that of f MLP and LTB4 in speed and direction (P0.001). The chemotactic effect of high concentration of f MLP on neutrophils was weakened, and the chemotactic effect of PAO1 d 5 was disappeared after dilution 50 times. PAO1 d 5 could inhibit the chemotaxis of neutrophils induced by f MLP. Conclusion there is an inhibitor of neutrophil chemotaxis in PAO1d5 capsule.
【作者單位】： 血液病醫(yī)院 中國(guó)科學(xué)院南海海洋研究所
【基金】：國(guó)家自然科學(xué)基金(31271484,31471116)
【分類號(hào)】：R392

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