廣西紅水河流域長壽人群CETP TaqIB和I405V基因多態(tài)性與血脂和長壽相關(guān)性的研究
發(fā)布時間：2019-06-22 20:05
【摘要】：背景： 膽固醇酯轉(zhuǎn)移蛋白(cholesteryl ester transfer protein, CETP)在膽固醇逆向轉(zhuǎn)運中發(fā)揮關(guān)鍵作用,是脂類代謝中的重要調(diào)節(jié)蛋白。CETP是血漿HDL-C水平的最重要的決定因素之一,CETP基因發(fā)生改變可能引起血漿HDL-C水平的改變從而影響HDL和LDL的顆粒大小、血漿濃度,影響脂質(zhì)代謝,使心血管疾病的危險性升高,繼而影響壽命。CETP基因的多態(tài)性與血脂水平及長壽關(guān)系的研究已有一些報道,但目前尚未取得一致性的結(jié)論。 目的： 本研究主要探討廣西紅水河流域長壽人群CETP基因TaqIB和1405V位點的多態(tài)性,并探討其基因型與該地區(qū)血脂水平和長壽的關(guān)系。 方法： 應(yīng)用聚合酶鏈反應(yīng)-限制性片段長度多態(tài)性(PCR-RFLP)方法對廣西紅水河流域90歲以上壯族長壽老人523例(長壽組),普通健康壯族中老年人498例(對照組)進行CETP TaqIB和1405V位點的基因分型,并分析各基因型對血壓、體重指數(shù)(BMI)、總膽固醇(TC)、甘油三酯(TG)、高密度脂蛋白膽固醇(HDL-C)、低密度脂蛋白膽固醇(LDL-C)等指標(biāo)的影響,了解CETPTaqIB和I405V多態(tài)性與該群體血脂水平的關(guān)系及其在該地區(qū)長壽中的可能作用。 結(jié)果： 1.一般情況及血脂水平：長壽組血壓水平、高血壓率、TC、HDL-C、 LDL-C水平顯著高于對照組；而BMI、TG水平及血脂異常率低于對照組。 2.基因分型：CETP TaqIB等位基因在兩組均以B1為主,頻率60%；基因型則以野生型B1B1及雜合型B1B2為主。CETPI405V等位基因在兩組均以Ⅰ為主,約占58%,基因型以野生型Ⅱ和雜合型Ⅳ為主。CETP TaqIB和I405V兩位點的基因型頻率分布及等位基因頻率分布在長壽組和對照組間均未發(fā)現(xiàn)有顯著差異。但區(qū)分性別后組間比較分析顯示,對照組女性TaqIB B2等位基因頻率明顯低于長壽組女性(33.3vs39.1,P0.05)；在I405V位點,對照組男性和女性在基因型頻率分布方面存在顯著差異(p=0.008),大體表現(xiàn)為女性ⅤⅤ和Ⅱ基因型頻率高于男性(分別為20.4vs12.6,37.6vs32.4),而女性Ⅳ則低于男性(42.0vs54.9)。 3.基因型與BMI的關(guān)系：各組進一步區(qū)分為BMI24kg/m2(正常體型)和≥24kg/m2(超重)亞組,發(fā)現(xiàn)對照組超重率顯著高于長壽組(24.1%vs12.4%,P0.05)。在Taq IB位點,各組沒發(fā)現(xiàn)不同BMI在基因型頻率及等位基因頻率上的差異；而在I405V位點上,對照組超重者基因型ⅤⅤ及Ⅱ的頻率高于非超重者,而ⅣV則低于非超重者(P=0.011)。 4.基因型與血脂水平的關(guān)系：在Taq IB位點,組內(nèi)比較顯示,基因型為B2B2和B1B2的HDL-C水平均明顯高于B181的HDL-C水平(P0.05)。組間比較發(fā)現(xiàn),長壽組B1B1攜帶者的HDL-C水平及各種基因型的LDL-C水平顯著高于對照組(P0.05),區(qū)分性別后,長壽組男性的這種表現(xiàn)仍存在。在I405V位點,組內(nèi)比較顯示各血脂水平在不同基因型間無顯著差異,然而,組間比較發(fā)現(xiàn),對照組男性Ⅱ基因型的TC和LDL-C水平有高于Ⅳ和VV的趨勢,而女性無此差異。 5.線性回歸分析：合并人群的TC、TG水平與舒張壓、BMI呈正顯著相關(guān)(P0.01),與年齡呈負(fù)相關(guān)(P0.01～0.05),HDL-C水平與年齡、Taq IB呈顯著正相關(guān)(P0.01),與BMI和I405V呈負(fù)相關(guān)(P0.01～0.05),LDL-C與舒張壓呈顯著正相關(guān)(P0.01)。 結(jié)論： 1.廣西紅水河流域長壽人群與自然人群在一些血脂水平、血脂異常率、高血壓率等方面存在明顯差異。 2.總體上,CETP TaqIB和I405V兩位點的基因型頻率及等位基因頻率分布在長壽人群和自然人群間無差異。 3. CETP Taq IB B2等位基因攜帶者(包括基因型B2B2和B1B2)在處理膽固醇上有一定優(yōu)勢,主要表現(xiàn)為HDL-C增高。 4.紅水河流域的長壽與CETP基因多態(tài)有一定的關(guān)系,并存在性別差異,可能通過某些優(yōu)勢基因型影響血脂水平,尤其是HDL-C的水平,從而減少冠心病等老年相關(guān)性疾病的發(fā)生,延長壽命。
[Abstract]:Background: Cholesteryl ester transfer protein (CETP) plays a key role in the reverse transport of cholesterol, and is an important regulation egg in lipid metabolism. White. CETP is one of the most important determinants of plasma HDL-C levels. The changes in the CETP gene may cause a change in the plasma HDL-C level, thus affecting the size of the particles of HDL and LDL, the plasma concentration, the lipid metabolism, the risk of cardiovascular disease, and the longevity The study of the relationship between the polymorphism of CETP gene and the level of blood lipid and long-life has been reported. On the other hand. Objective: To study the polymorphism of CETP gene TaqIB and 1405 V in the Longevity population of Hongshui River in Guangxi, and to explore its genotype and the level of blood lipid in the region. long-lived Methods: The polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method was used to study the effect of the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) on 523 cases (long-life group) and 498 cases (control group) of the strong and long-life old people in Hongshui River, Guangxi, and 498 cases (control group) of the general healthy and healthy people were treated with CETP TaqIB and 140. Genotyping of the 5 V locus, and the analysis of each genotype on blood pressure, body mass index (BMI), total cholesterol (TC), triglyceride (TG), high-density lipoprotein cholesterol (HDL-C), low-density lipoprotein cholesterol (LDL) -C) The effect of the indicators such as the relationship between the CETPTaqIB and the I405V polymorphism and the level of blood lipid in the population and their presence long life of the district Results:1. The level of blood pressure, blood pressure, TC, HDL-C and LDL-C in the long-life group were significantly higher than that in the control group. The level and the abnormal rate of dyslipidemia were lower than that of the control group.2. Genotyping: CETP TaqIB allele was dominant in both groups with frequency of 60%; genotype was wild The type B1B1 and the heterozygous B1B2 were the main. The CETPI405V allele was mainly in the first group and about 58% in both groups. Genotype was dominated by wild type 鈪，
本文編號：2504914
[Abstract]:Background: Cholesteryl ester transfer protein (CETP) plays a key role in the reverse transport of cholesterol, and is an important regulation egg in lipid metabolism. White. CETP is one of the most important determinants of plasma HDL-C levels. The changes in the CETP gene may cause a change in the plasma HDL-C level, thus affecting the size of the particles of HDL and LDL, the plasma concentration, the lipid metabolism, the risk of cardiovascular disease, and the longevity The study of the relationship between the polymorphism of CETP gene and the level of blood lipid and long-life has been reported. On the other hand. Objective: To study the polymorphism of CETP gene TaqIB and 1405 V in the Longevity population of Hongshui River in Guangxi, and to explore its genotype and the level of blood lipid in the region. long-lived Methods: The polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method was used to study the effect of the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) on 523 cases (long-life group) and 498 cases (control group) of the strong and long-life old people in Hongshui River, Guangxi, and 498 cases (control group) of the general healthy and healthy people were treated with CETP TaqIB and 140. Genotyping of the 5 V locus, and the analysis of each genotype on blood pressure, body mass index (BMI), total cholesterol (TC), triglyceride (TG), high-density lipoprotein cholesterol (HDL-C), low-density lipoprotein cholesterol (LDL) -C) The effect of the indicators such as the relationship between the CETPTaqIB and the I405V polymorphism and the level of blood lipid in the population and their presence long life of the district Results:1. The level of blood pressure, blood pressure, TC, HDL-C and LDL-C in the long-life group were significantly higher than that in the control group. The level and the abnormal rate of dyslipidemia were lower than that of the control group.2. Genotyping: CETP TaqIB allele was dominant in both groups with frequency of 60%; genotype was wild The type B1B1 and the heterozygous B1B2 were the main. The CETPI405V allele was mainly in the first group and about 58% in both groups. Genotype was dominated by wild type 鈪，
本文編號：2504914
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