【摘要】：目的：研究高溫對(duì)鼠胚神經(jīng)管發(fā)育時(shí)期神經(jīng)上皮細(xì)胞的粘附聚集、增殖凋亡及細(xì)胞生長(zhǎng)因子的影響,從而探討高溫致神經(jīng)管畸形的發(fā)生機(jī)理。方法：將孕鼠隨機(jī)分成為實(shí)驗(yàn)組30只和對(duì)照組25只,實(shí)驗(yàn)組鼠于孕8d下午15：30進(jìn)行42℃水浴20min,對(duì)照組鼠置于37℃水浴箱20min。實(shí)驗(yàn)組和對(duì)照組各10只孕鼠于孕15d處死剖腹取胎,辨認(rèn)和計(jì)數(shù)活胎、死胎、吸收胎和畸胎。其余孕鼠35只,分別于37℃和42°C水浴后2h、8h、16h、24h、48h處死,剖腹取胎,置于10%的中性福爾馬林液中固定,常規(guī)石蠟包埋、連續(xù)切片。用免疫組織化學(xué)法和圖像分析儀檢測(cè)N-cadherin、bFGF、PCNA在各期實(shí)驗(yàn)組和對(duì)照組鼠胚神經(jīng)管上皮細(xì)胞的表達(dá)變化。結(jié)果：①實(shí)驗(yàn)組鼠胚神經(jīng)管畸形率達(dá)70.91%,對(duì)照組致畸率為0。②N-cadherin的免疫組織化學(xué)結(jié)果：水浴后2-8h N-cadherin在對(duì)照組及實(shí)驗(yàn)組神經(jīng)管上皮細(xì)胞表達(dá)呈陽性,二者之間的OD值差異無統(tǒng)計(jì)學(xué)意義,內(nèi)外界膜、脊索及神經(jīng)管周圍間充質(zhì)細(xì)胞也可見陽性反應(yīng)。水浴后16-24h, N-cadherin在對(duì)照組神經(jīng)管上皮細(xì)胞表達(dá)呈強(qiáng)陽性,而在實(shí)驗(yàn)組表達(dá)呈弱陽性,OD值差異有統(tǒng)計(jì)學(xué)意義(p0.01),周圍組織陽性變化不明顯。至水浴后48h實(shí)驗(yàn)組與對(duì)照組相比,神經(jīng)上皮N-cadherin免疫陽性反應(yīng)差異不明顯,N-cadherin蛋白在兩組神經(jīng)管組織表達(dá)均減弱。③)bFGF的免疫組織化學(xué)結(jié)果：水浴后2h、8h、16h bFGF蛋白在實(shí)驗(yàn)組和對(duì)照組神經(jīng)上皮中表達(dá),兩組OD值差異無統(tǒng)計(jì)學(xué)意義,水浴后24h-48h實(shí)驗(yàn)組神經(jīng)上皮的表達(dá)弱于對(duì)照組,且OD值差異有統(tǒng)計(jì)學(xué)意義(p0.05), bFGF在兩組神經(jīng)管上皮的表達(dá)隨胚齡增加有增強(qiáng)的趨勢(shì),內(nèi)外界膜、脊索和周圍間充質(zhì)也呈現(xiàn)陽性反應(yīng)。④孕8d, PCNA蛋白廣泛表達(dá)在神經(jīng)管組織,神經(jīng)管上皮細(xì)胞、神經(jīng)嵴細(xì)胞、周圍間充質(zhì)細(xì)胞均呈現(xiàn)高表達(dá)狀態(tài)。水浴后2h和8h, PCNA在實(shí)驗(yàn)組神經(jīng)組織的陽性表達(dá)數(shù)低于對(duì)照組,但差異無統(tǒng)計(jì)學(xué)意義,高溫處理后16h、24h和48h PCNA在實(shí)驗(yàn)組神經(jīng)管上皮組織的陽性細(xì)胞數(shù)低于對(duì)照組,且差異有統(tǒng)計(jì)學(xué)意義(p0.05)。水浴處理24h之后,PCNA在實(shí)驗(yàn)組和對(duì)照組神經(jīng)組織中的陽性細(xì)胞數(shù)均有明顯下降的趨勢(shì)。結(jié)論：高溫可使N-cadherin、bFGF、PCNA在神經(jīng)管上皮細(xì)胞的表達(dá)降低,影響細(xì)胞間的識(shí)別、粘附,干擾細(xì)胞增殖與凋亡的動(dòng)態(tài)平衡,誘導(dǎo)NTDs發(fā)生,是高溫導(dǎo)致神經(jīng)管缺陷的重要途徑之一。
[Abstract]:Aim: to study the effects of high temperature on adhesion and aggregation, proliferation, apoptosis and cell growth factor of neural epithelial cells during the development of neural tube in mouse embryo, so as to explore the mechanism of neural tube malformation induced by high temperature. Methods: the pregnant rats were randomly divided into experimental group (n = 30) and control group (n = 25). The rats in the experimental group were bathed at 42 鈩，

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