【摘要】：目的通過抑制Ppif基因的表達(dá),觀察其對(duì)實(shí)驗(yàn)大鼠缺血再灌注損傷腎臟所起的保護(hù)效果,并分析、討論其作用原理。 方法首先用大鼠建立腎缺血再灌注模型,并隨機(jī)將實(shí)驗(yàn)大鼠分組(共3組):空白對(duì)照組(NC Group)、陰性對(duì)照組(CON Group)、治療組(Treated Group)(每組各20只大鼠)。治療組大鼠再灌注時(shí)注射Ppif基因靶向RNAi慢病毒載體,劑量0.3 mL(4μg/ g),陰性對(duì)照組再灌注時(shí)注射0.3 mL生理鹽水(含體積分?jǐn)?shù)0.01 DMSO,即二甲基亞砜),空白對(duì)照組不處理腎蒂,即腎臟未經(jīng)歷缺血再灌注,其余處理措施按陰性對(duì)照組方法進(jìn)行。達(dá)預(yù)定時(shí)間后,分別用相應(yīng)方法檢測(cè)各實(shí)驗(yàn)組大鼠細(xì)胞凋亡指數(shù)(AI)、血肌酐(Cr)水平、血尿素氮(BUN)水平、HE染色細(xì)胞病理切片檢查。 結(jié)果治療組大鼠相對(duì)于陰性對(duì)照組大鼠,血液中Cr和BUN測(cè)定數(shù)值均明顯下降,AI明顯減低,二者的差異均有統(tǒng)計(jì)學(xué)意義(P 0.05)。病理學(xué)檢查結(jié)果(HE組織染色方法)顯示:3組大鼠對(duì)比,治療組大鼠缺血明顯減輕。 結(jié)論大鼠Ppif基因的表達(dá)能夠被Ppif基因靶向RNAi慢病毒載體有效抑制,從而達(dá)到抑制線粒體途徑凋亡的目的,最終當(dāng)大鼠腎臟缺血再灌注出現(xiàn)時(shí),起到對(duì)缺血腎臟有效的保護(hù)作用。
[Abstract]:Objective to observe the protective effect of Ppif gene on renal ischemia-reperfusion injury in rats by inhibiting the expression of VEGF gene, and to analyze and discuss its principle of action. Methods the model of renal ischemia-reperfusion was established by using rats, and the experimental rats were randomly divided into three groups: blank control group (NC Group), negative control group (CON Group), treatment group (Treated Group) (each group 20 rats). The rats in the treatment group were injected with Ppif gene targeting RNAi lentivirus vector at a dose of 0.3 mL (4 渭 g / g), negative control group). 0.3 mL saline (containing volume fraction 0.01 DMSO, dimethyl sulfoxide) was injected into the treatment group. The blank control group did not deal with the renal pedicle, that is, the kidney did not experience ischemia-reperfusion, and the other treatment measures were carried out according to the negative control group. After reaching the predetermined time, the apoptosis index (AI), serum creatinine (Cr) level, blood urea nitrogen (BUN) level and HE staining cell pathological section were measured by corresponding methods. Results compared with the negative control group, the values of Cr and BUN in the blood of the treatment group were significantly lower than those of the negative control group, and the AI of the treatment group was significantly lower than that of the negative control group (P 0.05). The results of pathological examination (HE tissue staining) showed that the ischemia of the rats in the treatment group was significantly alleviated compared with that in the three groups. Conclusion the expression of Ppif gene in rats can be effectively inhibited by Ppif gene targeting RNAi lentivirus vector, so as to inhibit the apoptosis of mtDNA pathway, and finally play an effective protective effect on ischemic kidney when ischemia-reperfusion occurs in rat kidney.
【學(xué)位授予單位】：華中科技大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2011
【分類號(hào)】：R363

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