【摘要】：目的：從紅細(xì)胞膜分子層次探討大鼠寒冷刺激血瘀模型、饑餓加力竭游泳血瘀模型、聲光電復(fù)合刺激加鉗尾激惹血瘀模型在血液流變學(xué)方面的生物學(xué)差異；應(yīng)用少腹逐瘀湯、補(bǔ)陽(yáng)還五湯、丹參飲反證寒冷刺激血瘀模型紅細(xì)胞膜生物學(xué)特征 方法：以大鼠為研究對(duì)象。(1)用冰水浸泡模擬寒冷刺激而致血瘀模型、以饑餓加力竭游泳模擬氣虛血瘀模型、以不可預(yù)期的聲光電復(fù)合刺激加鉗尾激惹模擬驚恐、憤怒過(guò)度而致氣滯血瘀模型。持續(xù)刺激直至2周后各組大鼠分別出現(xiàn)寒凝血瘀、氣虛血瘀、氣滯血瘀表征,頸動(dòng)脈取血測(cè)血液黏度、紅細(xì)胞變形性、血沉K值、凝血四項(xiàng)、血漿膽固醇及甘油三酯含量；取血制備紅細(xì)胞膜,檢測(cè)紅細(xì)胞膜Na+-K+-ATP酶活力、唾液酸含量、巰基含量、膽固醇含量、SOD活性、MDA含量。(2)寒冷刺激血瘀模型大鼠分別灌胃給予高、低劑量少腹逐瘀湯,高、低劑量補(bǔ)陽(yáng)還五湯及高、低劑量丹參飲,連續(xù)2周,頸動(dòng)脈取血,除檢測(cè)上述指標(biāo)外,以激光共聚焦顯微鏡觀察各組大鼠紅細(xì)胞膜骨架結(jié)構(gòu),并檢測(cè)紅細(xì)胞膜蛋白組成及百分含量。 結(jié)果：(1)與空白對(duì)照比較,寒冷刺激血瘀模型組全血黏度、血沉K值、FIB含量均顯著增高,紅細(xì)胞變形能力明顯降低,APTT明顯縮短,紅細(xì)胞膜Na+-K+-ATP酶活力、SOD活性顯著下降,唾液酸含量顯著降低(P0.05或P0.01)；饑餓加力竭游泳血瘀模型高切變率全血黏度、紅細(xì)胞變形性降低,APTT及PT明顯縮短,血漿膽固醇含量下降,紅細(xì)胞膜紅細(xì)胞膜Na+-K+-ATP酶活力及SOD活性顯著降低(P0.05或P0.01)；不可預(yù)期聲光電刺激加鉗尾激惹血瘀大鼠APTT明顯縮短,紅細(xì)胞膜Na+-K+-ATP酶活力及SOD活性顯著下降、唾液酸含量顯著降低,MDA含量增加(P0.05或P0.01)。(2)與寒冷刺激血瘀模型組比較,少腹逐瘀湯、補(bǔ)陽(yáng)還五湯、丹參飲均可顯著降低低切變率全血黏度,在高切變率下全血黏度少腹逐瘀湯降低效果最好；三方均能顯著升高紅細(xì)胞變形指數(shù)顯著降低FIB含量,增加紅細(xì)胞膜上唾液酸的含量及SOD酶活力(P0.05或P0.01)；丹參飲可顯著降低K值(P0.05),升高紅細(xì)胞膜帶1蛋白百分含量；少腹逐瘀湯可顯著糾正APTT縮短及降低MDA含量,提高Na+-K+-ATP酶活力,明顯改善F-actin解聚狀態(tài),升高紅細(xì)胞膜上帶1蛋白、帶2蛋白、帶3蛋白百分含量(P0.05或P0.01)。 結(jié)論：(1)宏觀血液流變學(xué)方面：寒冷刺激血瘀模型血液流變學(xué)呈高流變性特征；饑餓加力竭游泳血瘀模型血液流變學(xué)呈現(xiàn)低流變特點(diǎn)；聲光電復(fù)合刺激加鉗尾激惹血瘀模型除APTT明顯縮短,其余宏觀血液流變學(xué)指標(biāo)變化不明顯；(2)三種血瘀模型均表現(xiàn)為紅細(xì)胞膜Na+-K+-ATP酶活性、SOD活性降低；寒冷刺激和聲光電復(fù)合刺激血瘀模型紅細(xì)胞膜唾液酸含量減少；(3)補(bǔ)陽(yáng)還五湯、少腹逐瘀湯、丹參飲三方可糾正寒冷刺激血瘀模型全血黏度增高、紅細(xì)胞變形性降低、聚集性增強(qiáng)、纖維蛋白原含量增加及膜骨架構(gòu)象異常；糾正APTT縮短、提高膜Na+-K+-ATP酶活性、減少膜MDA含量方面,少腹逐瘀湯優(yōu)于其他兩方；少腹逐瘀湯可糾正寒冷刺激血瘀模型紅細(xì)胞膜帶1蛋白、帶2蛋白、帶3蛋白百分含量異常,丹參飲作用于膜帶1蛋白、帶7蛋白。
[Abstract]:Objective: To study the biological difference of the blood stasis model of the rat cold-stimulated blood stasis model, the starvation and the exhaustive swimming and blood stasis model and the acoustic-photoelectrical composite stimulation of the blood stasis model in the blood rheology from the molecular level of the red cell membrane. The biological characteristics of red cell membrane in the model of cold stimulation and blood stasis of Danshen Yin Methods: The rats were used as the research group. The method comprises the following steps of: (1) soaking in ice water to simulate the blood stasis model due to cold stimulation, and simulating the blood stasis model by using the hunger and exhausted swimming to simulate the blood stasis model, Type: After 2 weeks of continuous stimulation, the rats in each group had cold and blood stasis, Qi deficiency and blood stasis, qi stagnation and blood stasis, blood viscosity, erythrocyte deformability, erythrocyte sedimentation rate, blood coagulation, blood coagulation, plasma cholesterol and triglyceride content, and blood-taking preparation of red blood cells. The membrane was used to detect the activity of Na +-K +-ATPase in erythrocyte membrane, the content of sialic acid, the content of sodium peroxide, the content of cholesterol, the activity of SOD and the content of MDA. (2) In cold-stimulated and blood-stasis model rats, the rats were given high, low-dose, low-dose, no-blood-stasis decoction, high-dose, low-dose Buyang Huanwu decoction and high-dose, low-dose red-rooted salvia, for 2 weeks, the carotid artery was taken for blood, and the above-mentioned indexes were detected. In addition, the structure of red cell membrane was observed by laser confocal microscope and the composition and percentage of red blood cell membrane protein were detected. Results: (1) Compared with the blank control, the whole blood viscosity, the erythrocyte sedimentation rate and the FIB content of the model group were significantly higher than that of the blank control, and the erythrocyte deformability was significantly reduced, the APTT was obviously shortened, the Na +-K +-ATPase activity and the activity of SOD in the erythrocyte membrane were significantly shortened. There was a significant decrease in the content of sialic acid (P0.05 or P0.01), the high shear rate of the whole blood viscosity, the decrease of erythrocyte deformability, the decrease of APTT and PT, the decrease of plasma cholesterol, the decrease of erythrocyte membrane Na +-K +-ATPase activity and the activity of SOD (P0.05 or P0). The activity of Na +-K +-ATPase and the activity of SOD and the content of sialic acid decreased significantly, and the content of MDA increased (P0.05 or P0). (1). (2) Compared with the cold-stimulated blood stasis model group, the whole blood viscosity of the whole blood with low shear rate can be obviously reduced, the whole blood viscosity of the whole blood is reduced, the blood viscosity of the whole blood is reduced and the effect of the blood stasis decoction is reduced under the high shear rate, The results showed that the red blood cell deformation index significantly decreased the content of the FIB, increased the content of the sialic acid on the erythrocyte membrane and the activity of the SOD (P0.05 or P0.01). The content of APTT and the content of MDA, Na +-K +-ATPase activity were significantly improved, the activity of Na +-K +-ATPase was improved, the depolymerized state of F-actin was obviously improved, and 1 protein,2-protein and 3-protein in the erythrocyte membrane were increased (P0.05 or P0). 01). Conclusion: (1) The blood rheology in the model of blood rheology in cold-stimulated blood-stasis model is characterized by high rheological property, and the blood rheology of the model is characterized by low rheology. (2) The activity of Na +-K +-ATPase and the decrease of SOD activity of erythrocyte membrane Na +-K +-ATPase were observed in the three blood stasis models. The blood viscosity of the whole blood of the cold-stimulated blood-stasis model can be corrected by the three-way of the lower abdomen and the blood-stasis decoction, the deformability of the red blood cell is reduced, the aggregation is enhanced, the content of the fibrinogen is increased, and the conformation of the membrane skeleton is abnormal; the APTT is shortened, the Na +-K +-ATPase activity of the membrane is improved, the membrane MD is reduced, In the aspect of content A, the lower abdomen and the blood stasis decoction is superior to the other two; the lower abdomen and the blood stasis decoction can correct the red cell membrane band 1 protein, the 2 protein and the 3 protein content of the red cell membrane of the cold stimulation blood stasis model, the red sage root drink is applied to the membrane band 1 protein,
【學(xué)位授予單位】：黑龍江中醫(yī)藥大學(xué)
【學(xué)位級(jí)別】：博士
【學(xué)位授予年份】：2012
【分類號(hào)】：R-332

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