【摘要】：目的觀察脊髓水平5-羥色胺受體參與谷氨酸微注射激活延髓外側(cè)網(wǎng)狀核對(duì)大鼠心臟傷害性感受的下行性調(diào)控作用。方法雄性SD大鼠隨機(jī)分為5組:緩激肽組、緩激肽+谷氨酸組、緩激肽+麥角新堿組、緩激肽+谷氨酸+麥角新堿組、緩激肽+谷氨酸+溶媒組。谷氨酸微注射于延髓外側(cè)網(wǎng)狀核,聯(lián)合鞘內(nèi)注射,觀察心包內(nèi)緩激肽誘發(fā)大鼠心臟-軀體運(yùn)動(dòng)反射的變化,該反射以背斜方肌肌電(EMG)為觀測(cè)指標(biāo);同時(shí)觀察脊髓背角c-Fos的表達(dá)。結(jié)果(1)與緩激肽組相比,鞘內(nèi)注射5-羥色胺受體拮抗劑麥角新堿對(duì)EMG活動(dòng)無(wú)影響,然而,谷氨酸微注射激活延髓外側(cè)網(wǎng)狀核劑量依賴性地抑制了心包內(nèi)緩激肽誘發(fā)的EMG活動(dòng);同時(shí),脊髓背角c-Fos表達(dá)顯著減少(P0.05);(2)與緩激肽+谷氨酸組相比,鞘內(nèi)注射麥角新堿后,部分反轉(zhuǎn)了化學(xué)激活延髓外側(cè)網(wǎng)狀核對(duì)EMG活動(dòng)的抑制作用;同時(shí),脊髓背角c-Fos表達(dá)增加(P0.05)。然而,鞘內(nèi)注射溶媒后,心包內(nèi)緩激肽誘發(fā)的EMG活動(dòng)與脊髓背角c-Fos的表達(dá)無(wú)統(tǒng)計(jì)學(xué)意義(P0.05)。結(jié)論脊髓水平的5-羥色胺受體參與了延髓外側(cè)網(wǎng)狀核對(duì)大鼠心臟傷害性感受的下行性抑制調(diào)控作用。
[Abstract]:Objective to observe the role of serotonin receptor at spinal cord level in the downward regulation of cardiac nociceptive perception in rats activated by glutamic acid microinjection to activate the lateral reticular medulla oblongata. Methods male SD rats were randomly divided into 5 groups: bradykinin group, bradykinin glutamic acid group, bradykinin ergoxin group and bradykinin glutamic acid solvent group. Glutamate microinjection into the lateral reticular nucleus of medulla oblongata, combined with intrathecal injection, was used to observe the changes of cardiac-somatic motor reflex induced by intrapericardial bradykinin in rats. The electromyography (EMG) of antioblique muscle was used as the index of the reflex. At the same time, the expression of c-Fos in spinal dorsal horn was observed. Results (1) compared with bradykinin group, intrathecal injection of 5-hydroxytryptamine receptor antagonist ergoxin had no effect on EMG activity. Glutamate microinjection activated the lateral reticular nucleus of medulla oblongata in a dose-dependent manner to inhibit the activity of EMG induced by bradykinin in pericardium. At the same time, the expression of c-Fos in spinal dorsal horn was significantly decreased compared with bradykinin glutamate group (P 0.05); (2). After intrathecal injection of ergoxin, the inhibitory effect of chemical activation of lateral reticular of medulla oblongata on EMG activity was partially reversed. At the same time, the expression of c-Fos in spinal dorsal horn increased (P 0.05). However, after intrathecal injection of solvent, there was no significant difference in EMG activity induced by pericardial bradykinin and the expression of c-Fos in spinal dorsal horn (P 0.05). Conclusion 5-hydroxytryptamine receptor at spinal cord level is involved in the downward inhibitory regulation of cardiac nociceptive perception in rats with lateral reticular nucleus oblongata.
【作者單位】： 陜西中醫(yī)藥大學(xué)生理學(xué)教研室;西安交通大學(xué)醫(yī)學(xué)部生理學(xué)與病理生理學(xué)系;
【基金】：國(guó)家自然科學(xué)基金(31171068) 陜西省教育廳自然科學(xué)基金(320104-203010003,20140736) 陜西中醫(yī)藥大學(xué)博士基金(504010223)~~
【分類號(hào)】：R338

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