【摘要】：目的:探討脛骨內(nèi)注射法建立的大鼠乳腺癌骨轉(zhuǎn)移疼痛模型骨病灶的影像學確認方法。方法:將Walker 256大鼠乳腺癌腹水瘤注射入Wistar大鼠脛骨腔內(nèi),制作乳腺癌骨癌痛模型;以脛骨內(nèi)注射等量PBS溶液作為對照組。使用von-Frey法,測量大鼠后足機械痛覺閾值,觀察各組疼痛變化。對模型組和對照組進行~(99mTc)-MDP骨顯像和CT成像,觀察骨病灶的骨代謝變化及骨質(zhì)結(jié)構的變化。結(jié)果:造模的第3~5天,乳腺癌骨轉(zhuǎn)移疼痛大鼠的機械痛覺閾值較基礎值明顯下降(P0.01),并持續(xù)下降83.2%左右,至第9天左右達到最痛(P0.001),后下降曲線趨于緩慢變化。造模3~5天后,模型組機械痛覺閾值顯著低于對照組(P0.01),這個差別持續(xù)增大至第9天左右。~(99mTc)-MDP骨顯像顯示所有模型組大鼠脛骨種植腫瘤細胞處放射性攝取顯著高于對側(cè),CT見造模側(cè)脛骨上段骨質(zhì)破壞,對側(cè)骨質(zhì)結(jié)構正常,未見其他部位骨病灶存在。而對照組所有大鼠骨顯像示兩側(cè)脛骨放射性分布對稱,CT顯示兩側(cè)骨質(zhì)結(jié)構正常。結(jié)論:脛骨內(nèi)注射法大鼠骨癌痛模型疼痛確切。~(99mTc)-MDP骨顯像以及CT可以從骨代謝和骨結(jié)構評估骨癌痛模型病灶的發(fā)生,是確認此類骨轉(zhuǎn)移疼痛模型造模的成功較好的影像學方法。
[Abstract]:Objective: to investigate the imaging confirmation of bone lesions in rat breast cancer bone metastasis pain model established by intra-tibia injection. Methods: Walker 256 rats were injected into the tibia cavity of Wistar rats to establish the model of breast cancer pain, and the same amount of PBS solution was injected into the tibia as the control group. The mechanical pain threshold of posterior foot of rats was measured by von-Frey method, and the changes of pain in each group were observed. ~ (99mTc)-MDP bone imaging and CT imaging were performed in the model group and the control group to observe the changes of bone metabolism and bone structure. Results: on the 3rd to 5th day, the mechanical pain threshold of rats with bone metastasis pain of breast cancer was significantly lower than that of the basic value (P0.01), and continued to decrease by 83.2%, reaching the highest pain on the 9th day (P0.001). The post-decline curve tends to change slowly. After 5 days of modeling, the threshold of mechanical pain in the model group was significantly lower than that in the control group (P 0.01). The difference continued to increase until the 9th day. ~ (99mTc)-MDP bone imaging showed that the radioactivity uptake of tumor cells in the tibia of all model groups was significantly higher than that of the contralateral side. CT showed bone destruction in the upper tibia of the model side, and the contralateral bone structure was normal. No bone lesions were found in other parts. In the control group, the radioactivity distribution of both tibia was symmetrical on bone imaging, and the bone structure of both sides was normal on CT. Conclusion: the pain of bone cancer pain model in rats by intra-tibia injection is accurate. ~ (99mTc)-MDP bone imaging and CT can evaluate the occurrence of bone cancer pain model from bone metabolism and bone structure. It is a good imaging method to confirm the success of this kind of bone metastasis pain model.
【作者單位】： 復旦大學附屬華山醫(yī)院核醫(yī)學科;同濟大學附屬東方醫(yī)院核醫(yī)學科;復旦大學上海醫(yī)學院中西醫(yī)結(jié)合系;
【基金】：國家自然科學基金No.81201113 上海市衛(wèi)計委青年科學基金項目(20124Y069)~~
【分類號】：R-332;R737.9;R738.1

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