【摘要】：目的:分析番茄紅素對神經(jīng)病理性疼痛模型大鼠脊髓組織中疼痛相關(guān)分子表達(dá)的影響。方法:取健康雌性SD大鼠30只,根據(jù)文獻(xiàn)方法建立神經(jīng)病理性疼痛模型,包括假手術(shù)組、模型對照組、模型治療組各10只。將大鼠處死后獲取脊髓L2-L6段,測定其中疼痛相關(guān)指標(biāo)的血清水平、基因及蛋白表達(dá)情況。結(jié)果:假手術(shù)組大鼠的血清IL-17、HMGB-1、Aβ、Tau、C3水平低于模型對照組及模型治療組,CGRP水平高于模型對照組及模型治療組,模型治療組大鼠的血清IL-17、HMGB-1、Aβ、Tau、C3水平低于模型對照組,CGRP水平高于模型對照組(P0.05);假手術(shù)組大鼠的ERK、CREB、BDNF、NMDA、AMPA、c-fos mRNA表達(dá)量低于模型對照組及模型治療組,模型治療組大鼠的脊髓ERK、CREB、BDNF、NMDA、AMPA、c-fos mRNA表達(dá)量低于模型對照組大鼠(P0.05);假手術(shù)組大鼠的TRPV1、NF-κB、NOS、GFAP、ERK、CREB蛋白表達(dá)量低于模型對照組及模型治療組,Reg表達(dá)量高于模型對照組及模型治療組(P0.05),模型治療組大鼠的TRPV1、NF-κB、NOS、GFAP、ERK、CREB蛋白表達(dá)量低于模型對照組,Reg表達(dá)量高于模型對照組(P0.05)。結(jié)論:番茄紅素可以有效減少神經(jīng)病理性疼痛模型大鼠的促疼痛基因表達(dá),有望成為日后神經(jīng)病理性疼痛治療的新手段。
[Abstract]:Aim: to analyze the effect of lycopene on the expression of pain-related molecules in spinal cord of neuropathic pain model rats. Methods: thirty healthy female SD rats were used to establish neuropathic pain models, including sham operation group, model control group and model treatment group (n = 10). After the rats were killed, the L2-L6 segment of spinal cord was obtained and the serum level, gene and protein expression of pain-related indexes were measured. Results: the levels of serum IL-17,HMGB-1,A 尾 and Tau,C3 in sham operation group were lower than those in model control group and model treatment group, and the level of CGRP was higher than that in model control group and model treatment group. The serum IL-17,HMGB-1, level in model treatment group was higher than that in model control group. The levels of A 尾 and Tau,C3 were lower and the level of CGRP was higher than that of the model control group (P0.05). The expression of ERK,CREB,BDNF,NMDA,AMPA,c-fos mRNA in sham operation group was lower than that in model control group and model treatment group, and the spinal cord ERK,CREB,BDNF,NMDA,AMPA, expression in model treatment group was lower than that in model control group. The expression of c-fos mRNA was lower than that of the model control group (P0.05). The expression of TRPV1,NF- 魏 B and NOS,GFAP,ERK,CREB protein in sham operation group was lower than that in model control group and model treatment group, and the expression of Reg was higher than model control group and model treatment group (P0.05). The expression of TRPV1,NF- 魏 B in model treatment group was higher than that in model control group and model treatment group. The expression of NOS,GFAP,ERK,CREB protein was lower than that of model control group, and the expression of Reg was higher than model control group (P0.05). Conclusion: lycopene can effectively reduce the expression of pain-promoting gene in neuropathic pain model rats, and may become a new method for the treatment of neuropathic pain in the future.
【作者單位】： 廣東省深圳市中醫(yī)院特色診療�？�;
【基金】：廣東省衛(wèi)生廳醫(yī)學(xué)科研課題(A2011561)~~
【分類號】：R285.5;R-332

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