發(fā)布時間：2019-04-16 14:44

【摘要】：性發(fā)育是指個體性成熟并獲得生殖能力的過程,受到多種因素的影響。性發(fā)育的啟動時間是性發(fā)育過程中相當關(guān)鍵的一個性狀。該性狀是一個復雜性狀,在個體水平上受多種遺傳和環(huán)境因素的影響。 本研究之前的工作通過選擇性發(fā)育起始時間差異較大的兩種品系小鼠C3H/HeJ和C57BL/6作為親本,產(chǎn)生F7代。通過STR, SNP位標利用特異性區(qū)段同類系的家系構(gòu)建策略在小鼠X染色體上確定了一個和小鼠陰門開啟相關(guān)的的Rs13483770～Rs29055848之間～1cM(物理距離1.7Mb)的區(qū)間長度QTL區(qū)段。 本研究為了更進一步精細定位小鼠X染色體上與性發(fā)育相關(guān)的候選基因,針對發(fā)育較早的C3H/HeJ品系小鼠,將該QTL內(nèi)的12個候選基因(Mir505, Atp11c, Gm7073, Gm7076, Gm14661, Gm14662, Gm5637, Mcf2, F9, Fgf13, Gm715, Sox3)的編碼序列及其5’上游5k列進行了序列測定。利用Lasergene7.0軟件分析測序結(jié)果,首先通過SNP單倍型分析手段,初步排除了2個基因(Gm14662和F9)。后又進行反方向測序驗證,排除了3個無差異基因(Fgfl3, Gm5637, Mcf2)。利用之前本實驗室構(gòu)建的特異性區(qū)段同類系小鼠進行目的片段的重測序分析,選出4個候選基因(Mir505, Atp11c, Gm7073, Gm14661)。最后,結(jié)合本實驗室表型性狀差異較大的4個野老鼠品系(CM25, JS11, JS13, PD5)在以上候選基因處測序,按表型差異特點進行序列差異分析。將另外2個候選基因(Gm7073, Gm14661)逐一排除。此時,該區(qū)段內(nèi)與小鼠性發(fā)育啟動時間相關(guān)的候選基因被鎖定于Atp11c和Mir505。 本研究的結(jié)果初步確定了2個性發(fā)育啟動基因,為今后其相關(guān)機制的研究打下了良好的基礎。同時也有助于性發(fā)育啟動相關(guān)通路調(diào)節(jié)的研究。所有這些工作均有助于幫助我們理解性發(fā)育復雜性狀的整個生理過程和調(diào)控機制。
[Abstract]:Sexual development refers to the process of individual maturation and reproductive ability, which is influenced by many factors. The initiation time of sexual development is one of the most important traits in the process of sexual development. This trait is a complex trait which is influenced by many genetic and environmental factors at the individual level. In this study, two strains of mice, C3H/HeJ and C57BL/6, were used as parents to produce F7 generation. An interval length QTL region between Rs13483770~Rs29055848 and mouse vulvar opening-related Rs13483770~Rs29055848 (physical distance 1.7Mb) was determined on the X chromosome of mice by using the family construction strategy of the same family with specific regions by STR, SNP marker. In order to further map the candidate genes related to sexual development on the X chromosome of mice, 12 candidate genes (Mir505, Atp11c, Gm7073, Gm7076, Gm14661, Gm14662, Gm5637, Mcf2, F9, Mir505, Atp11c, Gm7073, Gm7076, Gm14661, Gm14662, Gm5637, Mcf2, F9, F9) in the QTL were selected for the early developing C3H/HeJ strain mice. The coding sequence of Fgf13, Gm715, Sox3) and its 5 'upstream 5k column were sequenced. Two genes (Gm14662 and F9) were initially excluded by SNP haplotype analysis using Lasergene7.0 software. Three indifference genes (Fgfl3, Gm5637, Mcf2) were excluded by reverse sequencing. Four candidate genes (Mir505, Atp11c, Gm7073, Gm14661) were selected by resequencing the target fragments from the mice of the same kind of specific region constructed in our laboratory. Finally, four strains of wild mice (CM25, JS11, JS13, PD5), which had great phenotypic differences in our laboratory, were sequenced at the above candidate genes, and the sequence differences were analyzed according to the phenotypic differences. The other two candidate genes (Gm7073, Gm14661) were excluded one by one. At this point, the candidate genes related to the initiation time of mouse sexual development in this region are locked to Atp11c and Mir505.. The results of this study provide a good basis for the further study of the related mechanism of personality development by identifying the promoter genes of personality development. At the same time, it is helpful to study the regulation of sexual development initiation-related pathway. All these work will help us to understand the whole physiological process and regulatory mechanism of complex traits of sexual development.
【學位授予單位】：東華大學
【學位級別】：碩士
【學位授予年份】：2011
【分類號】：R3416

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本文編號：2458861