鼠衣原體在不同遺傳背景小鼠中病變差異性與炎癥細胞動態(tài)變化
發(fā)布時間：2019-04-01 22:58
【摘要】：【目的】初步分析鼠衣原體(Chlamydia muridarum,Cm)致不同遺傳背景小鼠生殖道病變的原因,探討炎癥細胞動態(tài)變化在衣原體感染致不同人群病變差異的可能機制�！痉椒ā坑�2×105 Inclusion forming units(IFUs)/小鼠的Cm劑量經(jīng)子宮角(Intrauterine inoculation,iu)途徑分別感染DBA/2J鼠和A/J鼠;在感染60 d處死兩組小鼠,肉眼觀察其輸卵管水腫程度,顯微鏡下觀察評估輸卵管的擴張程度及炎癥反應(yīng)程度;每組小鼠在感染后3、7、14、21、28、35、42、49、56、60 d分別取陰道分泌物并檢測其中衣原體包涵體的數(shù)量;在第14天每組各處死5只小鼠,檢測上生殖道(輸卵管及卵巢)組織勻漿中衣原體包涵體數(shù)量及炎癥因子水平;同時,在3、28、35 d每組分別處死5只小鼠,顯微鏡下鑒定病變組織炎癥細胞種類及變化。【結(jié)果】兩組小鼠在感染60 d肉眼觀察下的生殖道病變率有明顯統(tǒng)計學(xué)差異,且兩組小鼠病變組織的輸卵管擴張程度也具有統(tǒng)計學(xué)差異,但炎癥反應(yīng)程度評分無統(tǒng)計學(xué)差異;DBA/2J與A/J兩組小鼠在陰道分泌物的衣原體包涵體檢測及小鼠陽性檢出率中并無顯著性差異;感染后14 d兩組小鼠在上生殖道組織勻漿中的包涵體檢出量無統(tǒng)計學(xué)差異,但某些炎癥因子水平具有統(tǒng)計學(xué)差異;感染早期兩組小鼠病變組織中嗜中性粒細胞浸潤增加,但無明顯差異,第28天DBA/2J小鼠病變組織中出現(xiàn)較多嗜酸性粒細胞,并在第35天與A/J小鼠形成統(tǒng)計學(xué)差異。【結(jié)論】DBA/2J與A/J小鼠在以iu方式感染等量Cm后,引起小鼠的生殖道病變差異可能與嗜酸性粒細胞參與的炎癥反應(yīng)有關(guān)。
[Abstract]:[objective] to analyze the causes of reproductive tract lesions caused by Chlamydia muridarum,Cm in mice with different genetic background. To explore the possible mechanism of the dynamic changes of inflammatory cells in different groups caused by chlamydia infection. [methods] DBA/ 2J mice and A / J mice were infected with 2 脳 10 5 Inclusion forming units (IFUs) / mouse Cm dose through the uterine horn (Intrauterine inoculation,iu) pathway, respectively. The mice of two groups were killed at 60 days after infection, and the degree of tubal edema was observed by naked eye, and the degree of tubal dilation and inflammatory reaction were observed and evaluated under microscope. At 3, 7, 14, 21, 28, 35, 42, 49, 56 days after infection, vaginal secretions of each group were taken and the number of the inclusion bodies of the chlamydia was detected respectively in the mice of each group at 3,7,14,21,28,35,42,49,56 days after infection. On the 14th day, 5 mice in each group were killed to detect the number of chlamydia inclusion bodies and the levels of inflammatory factors in the homogenate of the upper genital tract (oviduct and ovary). At the same time, 5 mice in each group were killed at 3,28,35 days to identify the types and changes of inflammatory cells in pathological tissue under microscope. [results] there was significant difference in reproductive tract pathological rate under naked eye observation on 60 days after infection between the two groups. The degree of tubal dilatation was also statistically different between the two groups of mice, but there was no statistical difference in the degree of inflammatory reaction. There was no significant difference between the two groups in the detection of coat body inclusion body in vaginal secretion and the positive detection rate of mice in the two groups of DBA/2J and AqJ. The results showed that there was no significant difference between the two groups. On the 14th day after infection, there was no statistical difference in the amount of physical examination in the homogenate of the upper genital tract between the two groups, but the levels of some inflammatory factors had statistical difference. In the early stage of infection, neutrophil infiltration increased, but there was no significant difference between the two groups. On the 28th day, there were more eosinophils in the pathological tissues of DBA/2J mice. [conclusion] the difference of reproductive tract pathological changes between DBA/2J and A / J mice may be related to the inflammatory reaction of eosinophils after the same amount of Cm was infected in the way of iu in mice on the 35th day. [conclusion] the difference of reproductive tract pathological changes in mice may be related to the inflammatory reaction involved in eosinophils.
【作者單位】： 南華大學(xué)醫(yī)學(xué)院病原生物學(xué)研究所;中南大學(xué)湘雅二醫(yī)院婦產(chǎn)科;中南大學(xué)湘雅二醫(yī)院病理科;
【基金】：國家自然科學(xué)基金項目(No.31570179,30901352) 湖南省自然科學(xué)基金項目(No.13JJ4072) 湖南省教育廳優(yōu)秀青年基金項目(No.12B109) 湖南省分子靶標新藥研究協(xié)同創(chuàng)新中心資助項目(No.2015-351) 特殊病原體防控湖南省重點實驗室資助項目(No.2014-5) 湖南省重點學(xué)科資助項目(No.2011-76)~~
【分類號】：R374
[Abstract]:[objective] to analyze the causes of reproductive tract lesions caused by Chlamydia muridarum,Cm in mice with different genetic background. To explore the possible mechanism of the dynamic changes of inflammatory cells in different groups caused by chlamydia infection. [methods] DBA/ 2J mice and A / J mice were infected with 2 脳 10 5 Inclusion forming units (IFUs) / mouse Cm dose through the uterine horn (Intrauterine inoculation,iu) pathway, respectively. The mice of two groups were killed at 60 days after infection, and the degree of tubal edema was observed by naked eye, and the degree of tubal dilation and inflammatory reaction were observed and evaluated under microscope. At 3, 7, 14, 21, 28, 35, 42, 49, 56 days after infection, vaginal secretions of each group were taken and the number of the inclusion bodies of the chlamydia was detected respectively in the mice of each group at 3,7,14,21,28,35,42,49,56 days after infection. On the 14th day, 5 mice in each group were killed to detect the number of chlamydia inclusion bodies and the levels of inflammatory factors in the homogenate of the upper genital tract (oviduct and ovary). At the same time, 5 mice in each group were killed at 3,28,35 days to identify the types and changes of inflammatory cells in pathological tissue under microscope. [results] there was significant difference in reproductive tract pathological rate under naked eye observation on 60 days after infection between the two groups. The degree of tubal dilatation was also statistically different between the two groups of mice, but there was no statistical difference in the degree of inflammatory reaction. There was no significant difference between the two groups in the detection of coat body inclusion body in vaginal secretion and the positive detection rate of mice in the two groups of DBA/2J and AqJ. The results showed that there was no significant difference between the two groups. On the 14th day after infection, there was no statistical difference in the amount of physical examination in the homogenate of the upper genital tract between the two groups, but the levels of some inflammatory factors had statistical difference. In the early stage of infection, neutrophil infiltration increased, but there was no significant difference between the two groups. On the 28th day, there were more eosinophils in the pathological tissues of DBA/2J mice. [conclusion] the difference of reproductive tract pathological changes between DBA/2J and A / J mice may be related to the inflammatory reaction of eosinophils after the same amount of Cm was infected in the way of iu in mice on the 35th day. [conclusion] the difference of reproductive tract pathological changes in mice may be related to the inflammatory reaction involved in eosinophils.
【作者單位】： 南華大學(xué)醫(yī)學(xué)院病原生物學(xué)研究所;中南大學(xué)湘雅二醫(yī)院婦產(chǎn)科;中南大學(xué)湘雅二醫(yī)院病理科;
【基金】：國家自然科學(xué)基金項目(No.31570179,30901352) 湖南省自然科學(xué)基金項目(No.13JJ4072) 湖南省教育廳優(yōu)秀青年基金項目(No.12B109) 湖南省分子靶標新藥研究協(xié)同創(chuàng)新中心資助項目(No.2015-351) 特殊病原體防控湖南省重點實驗室資助項目(No.2014-5) 湖南省重點學(xué)科資助項目(No.2011-76)~~
【分類號】：R374
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