【摘要】：液晶是一種介于液相和固相的中間相，，是自然界流動(dòng)性與有序性的有機(jī)結(jié)合，液晶態(tài)普遍存在于生物體內(nèi)。骨組織中的大多數(shù)礦物質(zhì)晶體的形成都和膠原纖維密切相關(guān)。系統(tǒng)研究液晶膠原的形成與功能及其與無(wú)機(jī)鈣磷鹽的有機(jī)復(fù)合，對(duì)開發(fā)新型仿生材料和人工組織器官研究有重要意義。 本研究從仿生角度出發(fā)，制備出膠原液晶膜。采用偏光顯微鏡、掃描電鏡和原子力顯微鏡研究膠原膜的液晶織構(gòu)和拓?fù)浣Y(jié)構(gòu)，通過MTT法、電鏡觀察、細(xì)胞骨架熒光染色觀察研究膠原液晶膜對(duì)小鼠成纖維細(xì)胞3T3及大鼠成骨細(xì)胞的黏附、增殖、生長(zhǎng)形貌及細(xì)胞骨架組裝的影響。通過細(xì)胞周期分析、堿性磷酸酶及骨鈣素測(cè)定進(jìn)一步研究膠原液晶膜對(duì)成骨細(xì)胞生長(zhǎng)及功能表達(dá)狀況的影響。另一方面研究膠原液晶膜在模擬體液中的礦化情況，探討鈣磷無(wú)機(jī)礦物在液晶態(tài)膠原基質(zhì)上沉積的過程和機(jī)理。結(jié)果表明，膠原液晶膜呈膽甾型液晶織構(gòu)，具有典型螺旋周期結(jié)構(gòu)的膠原纖維按一定方向自組裝，排列具有明顯取向性；與非液晶膜相比，膠原液晶膜不僅能更好的促進(jìn)3T3及成骨細(xì)胞的黏附、增殖及功能表達(dá)，且其拓?fù)浣Y(jié)構(gòu)對(duì)細(xì)胞生長(zhǎng)具有接觸引導(dǎo)效應(yīng)；膠原液晶膜獨(dú)特的膠原纖維結(jié)構(gòu)和排列影響無(wú)機(jī)鈣磷晶體的沉積和形成。
[Abstract]:Liquid crystal is a kind of intermediate phase between liquid phase and solid phase. Liquid crystal is an organic combination of fluidity and ordering in nature. Liquid crystal exists in organisms generally. The formation of most mineral crystals in bone tissue is closely related to collagen fibers. A systematic study on the formation and function of liquid crystal collagen and its organic compound with inorganic calcium and phosphorus salts is of great significance for the development of new biomimetic materials and the study of artificial tissues and organs. In this study, collagen liquid crystal membrane was prepared from bionic point of view. The texture and topological structure of collagen films were studied by polarized light microscope, scanning electron microscope and atomic force microscope. The effects of collagen liquid crystal membrane on adhesion, proliferation, growth morphology and cytoskeleton assembly of mouse fibroblast 3T3 and rat osteoblast were observed by cytoskeleton fluorescence staining. Cell cycle analysis, alkaline phosphatase (ALP) and osteocalcin (osteocalcin) were used to study the effects of collagen liquid crystalline membrane on the growth and functional expression of osteoblasts. On the other hand, the mineralization of collagen liquid crystal membrane in simulated body fluid was studied, and the process and mechanism of calcium and phosphorus inorganic mineral deposition on liquid crystalline collagen matrix were discussed. The results show that the collagen liquid crystal membrane is cholesteric liquid crystal texture, and the collagen fibers with typical spiral periodic structure are self-assembled in a certain direction and have obvious orientation. Compared with the non-liquid crystal membrane, the collagen liquid crystal membrane can not only promote the adhesion, proliferation and functional expression of 3T3 and osteoblasts, but also have contact-guiding effect on cell growth. The unique structure and arrangement of collagen fibers affect the deposition and formation of inorganic calcium and phosphorus crystals.
【學(xué)位授予單位】：暨南大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2012
【分類號(hào)】：R329

【參考文獻(xiàn)】

相關(guān)期刊論文 前10條

1 謝朝暉;陳蘭英;;細(xì)胞骨架研究進(jìn)展[J];癌變·畸變·突變;2011年04期

2 譚正德,蔡德福,譚松庭;熱致性液晶的合成表征[J];湖南工程學(xué)院學(xué)報(bào)(自然科學(xué)版);2004年01期

3 韓昭昭;孔樺;孟潔;王超英;朱廣瑾;解思深;許海燕;;取向納米纖維聚合物膜引導(dǎo)內(nèi)皮細(xì)胞生長(zhǎng)的作用[J];高等學(xué)�；瘜W(xué)學(xué)報(bào);2008年05期

4 劉強(qiáng);;液晶高分子材料及開發(fā)[J];甘肅科技;2006年10期

5 李云峰;細(xì)胞骨架與信號(hào)轉(zhuǎn)導(dǎo)[J];國(guó)外醫(yī)學(xué).藥學(xué)分冊(cè);1998年05期

6 趙蒼碧,黃玉東,李艷輝;從牛腱中提取膠原蛋白的研究[J];哈爾濱工業(yè)大學(xué)學(xué)報(bào);2004年04期

7 韓卓;陳曉燕;馬道榮;余順火;;激光掃描共聚焦顯微鏡實(shí)驗(yàn)技術(shù)與應(yīng)用[J];科技信息;2009年19期

8 果春青;任海云;;成蛋白:一種新的細(xì)胞微絲骨架組裝的調(diào)控因子[J];科學(xué)通報(bào);2006年19期

9 周峰;袁琳;黃鶴;陳紅;;納微米溝槽型圖案化表面“接觸引導(dǎo)”現(xiàn)象及其細(xì)胞生理效應(yīng)[J];科學(xué)通報(bào);2009年16期

10 夏露;梁星;王培志;王國(guó)平;;改良植塊法培養(yǎng)原代成骨細(xì)胞[J];南京醫(yī)科大學(xué)學(xué)報(bào)(自然科學(xué)版);2008年09期

相關(guān)碩士學(xué)位論文 前1條

1 吳芝燕;新型溶致液晶的構(gòu)建與表征[D];濟(jì)南大學(xué);2010年

本文編號(hào)：2451158