【摘要】：液晶是一種介于液相和固相的中間相，，是自然界流動性與有序性的有機結合，液晶態(tài)普遍存在于生物體內。骨組織中的大多數(shù)礦物質晶體的形成都和膠原纖維密切相關。系統(tǒng)研究液晶膠原的形成與功能及其與無機鈣磷鹽的有機復合，對開發(fā)新型仿生材料和人工組織器官研究有重要意義。 本研究從仿生角度出發(fā)，制備出膠原液晶膜。采用偏光顯微鏡、掃描電鏡和原子力顯微鏡研究膠原膜的液晶織構和拓撲結構，通過MTT法、電鏡觀察、細胞骨架熒光染色觀察研究膠原液晶膜對小鼠成纖維細胞3T3及大鼠成骨細胞的黏附、增殖、生長形貌及細胞骨架組裝的影響。通過細胞周期分析、堿性磷酸酶及骨鈣素測定進一步研究膠原液晶膜對成骨細胞生長及功能表達狀況的影響。另一方面研究膠原液晶膜在模擬體液中的礦化情況，探討鈣磷無機礦物在液晶態(tài)膠原基質上沉積的過程和機理。結果表明，膠原液晶膜呈膽甾型液晶織構，具有典型螺旋周期結構的膠原纖維按一定方向自組裝，排列具有明顯取向性；與非液晶膜相比，膠原液晶膜不僅能更好的促進3T3及成骨細胞的黏附、增殖及功能表達，且其拓撲結構對細胞生長具有接觸引導效應；膠原液晶膜獨特的膠原纖維結構和排列影響無機鈣磷晶體的沉積和形成。
[Abstract]:Liquid crystal is a kind of intermediate phase between liquid phase and solid phase. Liquid crystal is an organic combination of fluidity and ordering in nature. Liquid crystal exists in organisms generally. The formation of most mineral crystals in bone tissue is closely related to collagen fibers. A systematic study on the formation and function of liquid crystal collagen and its organic compound with inorganic calcium and phosphorus salts is of great significance for the development of new biomimetic materials and the study of artificial tissues and organs. In this study, collagen liquid crystal membrane was prepared from bionic point of view. The texture and topological structure of collagen films were studied by polarized light microscope, scanning electron microscope and atomic force microscope. The effects of collagen liquid crystal membrane on adhesion, proliferation, growth morphology and cytoskeleton assembly of mouse fibroblast 3T3 and rat osteoblast were observed by cytoskeleton fluorescence staining. Cell cycle analysis, alkaline phosphatase (ALP) and osteocalcin (osteocalcin) were used to study the effects of collagen liquid crystalline membrane on the growth and functional expression of osteoblasts. On the other hand, the mineralization of collagen liquid crystal membrane in simulated body fluid was studied, and the process and mechanism of calcium and phosphorus inorganic mineral deposition on liquid crystalline collagen matrix were discussed. The results show that the collagen liquid crystal membrane is cholesteric liquid crystal texture, and the collagen fibers with typical spiral periodic structure are self-assembled in a certain direction and have obvious orientation. Compared with the non-liquid crystal membrane, the collagen liquid crystal membrane can not only promote the adhesion, proliferation and functional expression of 3T3 and osteoblasts, but also have contact-guiding effect on cell growth. The unique structure and arrangement of collagen fibers affect the deposition and formation of inorganic calcium and phosphorus crystals.
【學位授予單位】：暨南大學
【學位級別】：碩士
【學位授予年份】：2012
【分類號】：R329

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