【摘要】：背景:力生長因子具有激活肌肉衛(wèi)星細(xì)胞和促進(jìn)肌原細(xì)胞生長的作用,同時在維持骨質(zhì)量和修復(fù)骨損傷方面具有作用。目的:研究力生長因子促進(jìn)兔骨髓間充質(zhì)干細(xì)胞向成骨細(xì)胞分化的作用機(jī)制。方法:采用MTT法檢測力生長因子促進(jìn)兔骨髓間充質(zhì)干細(xì)胞成骨分化的最佳質(zhì)量濃度與時間。用定量PCR和Western blot檢測此質(zhì)量濃度力生長因子促進(jìn)兔骨髓間充質(zhì)干細(xì)胞成骨分化后細(xì)胞中成骨細(xì)胞標(biāo)志物堿性磷酸酶和骨鈣素mRNA和蛋白的表達(dá)水平變化,并用Western Blot檢測AKT和mTOR磷酸化水平變化。結(jié)果與結(jié)論:力生長因子的質(zhì)量濃度為45μg/L時作用5 d促進(jìn)兔骨髓間充質(zhì)干細(xì)胞成骨分化的效果最好。45μg/L力生長因子促進(jìn)兔骨髓間充質(zhì)干細(xì)胞成骨分化后細(xì)胞中堿性磷酸酶和骨鈣素mRNA和蛋白水平在干預(yù)后4 h最高,同時AKT和mTOR的磷酸化水平也在干預(yù)后4 h時最高。說明力生長因子促進(jìn)兔骨髓間充質(zhì)干細(xì)胞向成骨細(xì)胞的分化的作用機(jī)制是通過PI3K/AKT途徑實現(xiàn)的,且在45μg/L干預(yù)后4 h時的效果最好。
[Abstract]:BACKGROUND: The force growth factor has the function of activating the muscle satellite cells and promoting the growth of the myogenic cells, and has the function of maintaining the bone quality and repairing the bone injury. Objective: To study the mechanism of force growth factor to promote the differentiation of bone marrow-derived mesenchymal stem cells into osteoblasts. Methods: The optimal mass concentration and time of bone marrow mesenchymal stem cells in rabbit bone marrow mesenchymal stem cells were measured by MTT assay. The expression of bone marrow mesenchymal stem cells in rabbit bone marrow-derived mesenchymal stem cells was detected by quantitative PCR and Western blot. The expression of bone marrow mesenchymal stem cells in rabbit bone marrow mesenchymal stem cells was changed, and the levels of AKT and mTOR phosphorylation were detected by Western Blot. Results and Conclusion: When the mass concentration of the force growth factor is 45. m u.g/ L, the effect of the function of 5 days to promote the osteogenic differentiation of the bone marrow mesenchymal stem cells of the rabbit is the best.45. mu. g/ L of the force growth factor promotes the development of the bone marrow mesenchymal stem cells in the rabbit, and the alkaline phosphatase and the bone-derived mRNA and the protein level in the cells are the highest after the intervention, At the same time, the phosphorylation of AKT and mTOR was also the highest at 4 h after intervention. The mechanism of the effect of force growth factor to promote the differentiation of bone marrow-derived mesenchymal stem cells into osteoblasts was achieved through the PI3K/ AKT pathway, and the effect was best at 4 h after 45. m u.g/ L intervention.
【作者單位】： 內(nèi)蒙古醫(yī)科大學(xué)第二附屬醫(yī)院;重慶醫(yī)科大學(xué)第一附屬醫(yī)院;
【分類號】：R329.2

【相似文獻(xiàn)】

相關(guān)期刊論文 前10條

1 郝慧斌;;對促進(jìn)燒傷愈合的生長因子正進(jìn)行動物試驗[J];生物技術(shù)通報;1987年10期

2 毛振奇;生長因子[J];生物學(xué)雜志;1987年05期

3 A.S.Goustin;陳虹;;生長因子與癌[J];細(xì)胞生物學(xué)雜志;1987年02期

4 李思經(jīng);;生長因子市場預(yù)測[J];生物技術(shù)通報;1988年08期

5 Michael B.Sporn;Anita B.Roberts;許仁林;;多功能的肽生長因子[J];世界科學(xué);1989年05期

6 張學(xué)賢;;多肽生長因子的生物學(xué)活性[J];國外畜牧科技;1992年04期

7 王承h，

本文編號：2448295