【摘要】：背景:力生長(zhǎng)因子具有激活肌肉衛(wèi)星細(xì)胞和促進(jìn)肌原細(xì)胞生長(zhǎng)的作用,同時(shí)在維持骨質(zhì)量和修復(fù)骨損傷方面具有作用。目的:研究力生長(zhǎng)因子促進(jìn)兔骨髓間充質(zhì)干細(xì)胞向成骨細(xì)胞分化的作用機(jī)制。方法:采用MTT法檢測(cè)力生長(zhǎng)因子促進(jìn)兔骨髓間充質(zhì)干細(xì)胞成骨分化的最佳質(zhì)量濃度與時(shí)間。用定量PCR和Western blot檢測(cè)此質(zhì)量濃度力生長(zhǎng)因子促進(jìn)兔骨髓間充質(zhì)干細(xì)胞成骨分化后細(xì)胞中成骨細(xì)胞標(biāo)志物堿性磷酸酶和骨鈣素mRNA和蛋白的表達(dá)水平變化,并用Western Blot檢測(cè)AKT和mTOR磷酸化水平變化。結(jié)果與結(jié)論:力生長(zhǎng)因子的質(zhì)量濃度為45μg/L時(shí)作用5 d促進(jìn)兔骨髓間充質(zhì)干細(xì)胞成骨分化的效果最好。45μg/L力生長(zhǎng)因子促進(jìn)兔骨髓間充質(zhì)干細(xì)胞成骨分化后細(xì)胞中堿性磷酸酶和骨鈣素mRNA和蛋白水平在干預(yù)后4 h最高,同時(shí)AKT和mTOR的磷酸化水平也在干預(yù)后4 h時(shí)最高。說(shuō)明力生長(zhǎng)因子促進(jìn)兔骨髓間充質(zhì)干細(xì)胞向成骨細(xì)胞的分化的作用機(jī)制是通過(guò)PI3K/AKT途徑實(shí)現(xiàn)的,且在45μg/L干預(yù)后4 h時(shí)的效果最好。
[Abstract]:BACKGROUND: The force growth factor has the function of activating the muscle satellite cells and promoting the growth of the myogenic cells, and has the function of maintaining the bone quality and repairing the bone injury. Objective: To study the mechanism of force growth factor to promote the differentiation of bone marrow-derived mesenchymal stem cells into osteoblasts. Methods: The optimal mass concentration and time of bone marrow mesenchymal stem cells in rabbit bone marrow mesenchymal stem cells were measured by MTT assay. The expression of bone marrow mesenchymal stem cells in rabbit bone marrow-derived mesenchymal stem cells was detected by quantitative PCR and Western blot. The expression of bone marrow mesenchymal stem cells in rabbit bone marrow mesenchymal stem cells was changed, and the levels of AKT and mTOR phosphorylation were detected by Western Blot. Results and Conclusion: When the mass concentration of the force growth factor is 45. m u.g/ L, the effect of the function of 5 days to promote the osteogenic differentiation of the bone marrow mesenchymal stem cells of the rabbit is the best.45. mu. g/ L of the force growth factor promotes the development of the bone marrow mesenchymal stem cells in the rabbit, and the alkaline phosphatase and the bone-derived mRNA and the protein level in the cells are the highest after the intervention, At the same time, the phosphorylation of AKT and mTOR was also the highest at 4 h after intervention. The mechanism of the effect of force growth factor to promote the differentiation of bone marrow-derived mesenchymal stem cells into osteoblasts was achieved through the PI3K/ AKT pathway, and the effect was best at 4 h after 45. m u.g/ L intervention.
【作者單位】： 內(nèi)蒙古醫(yī)科大學(xué)第二附屬醫(yī)院;重慶醫(yī)科大學(xué)第一附屬醫(yī)院;
【分類(lèi)號(hào)】：R329.2

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