【摘要】：目的研究大鼠發(fā)育過程中,生長休止蛋白7(growth arrest-specific protein 7,Gas7)在斜角帶核(diagonal band nucleus,DB)的表達。方法應用RT-PCR方法、Western blot方法和免疫組織化學方法觀察胚胎期16.5天(E16.5)、E20.5、出生當天(P0)、生后7天(P7)、P14、P21和2月齡(成年)大鼠DB的Gas7表達及定位。結果 RT-PCR和Western blot檢測顯示,在E16.5時Gas7表達最弱,其后逐漸增強,至P21時達到高峰;免疫組織化學染色顯示,斜角帶核水平支(horizontal limb of the diagonal band,HDB)在E16.5和E20.5時出現(xiàn)Gas7免疫反應陽性產物,Gas7陽性神經元出現(xiàn)于P0,至P21時陽性神經元數(shù)量最多,染色最深;斜角帶核垂直支(vertical limb ofthe diagonal band,VDB)至P14時Gas7陽性神經元最多,P21和成年均有所減少。結論 Gas7的表達在大鼠DB發(fā)育過程中具有時間和空間上的特異性,提示Gas7可能參與大鼠DB的發(fā)育過程。
[Abstract]:Aim to study the expression of growth rest protein 7 (growth arrest-specific protein 7, Gas 7) in diagonal band nucleus (diagonal band nucleus,DB) during development in rats. Methods RT-PCR, Western blot method and immunohistochemical method were used to observe the embryonic stage of 16.5 days (E16.5), E20.5, the day of birth (P0), postnatal day 7 (P7), P14, Expression and localization of Gas7 in DB of P21 and 2 month old rats. Results RT-PCR and Western blot showed that the expression of Gas7 was the weakest at E16.5, then increased gradually and reached the peak at P21. Immunohistochemical staining showed that (horizontal limb of the diagonal band,HDB in the horizontal branch of the diagonal band showed Gas7 immunoreactive products at E16.5 and E20.5, Gas7 positive neurons appeared in P0, and the number of positive neurons at P21 was the most, and the number of positive neurons at E16.5 and E20.5 was more than that at E16.5 and E20.5, respectively. The staining was the deepest; From the vertical branch of the diagonal band nucleus (vertical limb ofthe diagonal band,VDB) to P14, the number of Gas7-positive neurons was the most, while the number of P21 and adult neurons was decreased. Conclusion the expression of Gas7 has temporal and spatial specificity during the development of rat DB, suggesting that Gas7 may be involved in the development of DB in rats.
【作者單位】： 皖南醫(yī)學院人體解剖學教研室;
【基金】：安徽省高校省級自然科學項目(11040606M162) 安徽省名師工作室(2014msgzs 152) 皖南醫(yī)學院中青年科研基金項目(WK 201618)
【分類號】：R363

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